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  Indian J Med Microbiol
 

Figure 6: Photomicrograph of thoracic aortas from CN, HCD, ZI, ZII, ZIII, SG and ZSG groups symbolize immunoflourescent staining with RAM-11 antibody. The Control group shows no immunopositive reaction. HCD group demonstrates pronounced immunofluorescent positive reactive cells with increase intensity and percentage of greenish-fluorochrome foam cells in the intimal plaque (p). Aortas in ZPI, II and III groups demonstrate a significant reduction in the macrophage-derived foam cells within the tunica intima compare to HCD group, indicated by low intensity of greenish-fluorochrome immunopositive RAM-11 cells in the intimal plaque (p) (white arrows). However, ZSG group compared to SG group had showed significant lessening in the intensity and percentage of immunofluorescent positive area compare to HCD group. Bar graph represents IPFC and INNFC of macrophage-specific protein biomarker RAM-11. *indicates statistical significance compared to HCD at P < 0.05. L: Lumen; M: Media; A: Adventitia. Scale bars: 200 μm. CN: Control group; RAM-11: Regulation of ACE2 and morphogenesis 11; ZP: ZER-preventive; ZER: Zerumbone; IPC: Immunoreactive-positive cells; INC: Immunoreactive-negative cells; IPFC: Immunopositive fluorescent cells; INNFC: Immunonegative nonfluorescent cells

Figure 6: Photomicrograph of thoracic aortas from CN, HCD, ZI, ZII, ZIII, SG and ZSG groups symbolize immunoflourescent staining with RAM-11 antibody. The Control group shows no immunopositive reaction. HCD group demonstrates pronounced immunofluorescent positive reactive cells with increase intensity and percentage of greenish-fluorochrome foam cells in the intimal plaque (p). Aortas in ZPI, II and III groups demonstrate a significant reduction in the macrophage-derived foam cells within the tunica intima compare to HCD group, indicated by low intensity of greenish-fluorochrome immunopositive RAM-11 cells in the intimal plaque (p) (white arrows). However, ZSG group compared to SG group had showed significant lessening in the intensity and percentage of immunofluorescent positive area compare to HCD group. Bar graph represents IPFC and INNFC of macrophage-specific protein biomarker RAM-11. *indicates statistical significance compared to HCD at<i> P </i>< 0.05. L: Lumen; M: Media; A: Adventitia. Scale bars: 200 μm. CN: Control group; RAM-11: Regulation of ACE2 and morphogenesis 11; ZP: ZER-preventive; ZER: Zerumbone; IPC: Immunoreactive-positive cells; INC: Immunoreactive-negative cells; IPFC: Immunopositive fluorescent cells; INNFC: Immunonegative nonfluorescent cells