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  Indian J Med Microbiol
 

Figure 3: Effects of onjisaponin B on the expression of CaMKII and Cx43 protein of astrocytes at OGD 2 h (a) and OGD 4 h (b). The level of protein was shown by western blots (n = 3). The relative expression level of protein was determined with GAPDH densitometric intensity as the control. Effects of onjisaponin B on the expression of CaMKII mRNA (c) and Cx43 mRNA (d) of astrocytes at OGD 2 h and OGD 4 h. The level of mRNA was detected via qPCR (n = 3). The relative expression level of mRNA was determined with GAPDH by 2-ΔΔCt. (e) Effects of onjisaponin B on glutamate release of astrocytes at OGD 2 h and OGD 4 h. The level of Glu was determined via HPLC-MS. CK: Control group; OGD: Model group; 10 μM onjisaponin B: OGD + 10 μM onjisaponin B group; 20 μM onjisaponin B: OGD + 20 μM onjisaponin B group. ##P < 0.01 versus CK; ###P < 0.001 versus CK; *P < 0.05 versus OGD; **P < 0.01 versus OGD; ***P < 0.001 versus OGD. OGD: Oxygen and glucose deprivation; CaMKII: Calmodulin -dependent protein kinase II; HPLC-MS: High-performance liquid chromatography-tandem mass spectrometer

Figure 3: Effects of onjisaponin B on the expression of CaMKII and Cx43 protein of astrocytes at OGD 2 h (a) and OGD 4 h (b). The level of protein was shown by western blots (<i>n</i> = 3). The relative expression level of protein was determined with GAPDH densitometric intensity as the control. Effects of onjisaponin B on the expression of CaMKII mRNA (c) and Cx43 mRNA (d) of astrocytes at OGD 2 h and OGD 4 h. The level of mRNA was detected via qPCR (<i>n</i> = 3). The relative expression level of mRNA was determined with GAPDH by <sup>2-ΔΔCt</sup>. (e) Effects of onjisaponin B on glutamate release of astrocytes at OGD 2 h and OGD 4 h. The level of Glu was determined via HPLC-MS. CK: Control group; OGD: Model group; 10 μM onjisaponin B: OGD + 10 μM onjisaponin B group; 20 μM onjisaponin B: OGD + 20 μM onjisaponin B group. <sup>##</sup><i>P</i> < 0.01 versus CK; <sup>###</sup><i>P</i> < 0.001 versus CK; *<i>P</i> < 0.05 versus OGD; **<i>P</i> < 0.01 versus OGD; ***<i>P</i> < 0.001 versus OGD. OGD: Oxygen and glucose deprivation; CaMKII: Calmodulin -dependent protein kinase II; HPLC-MS: High-performance liquid chromatography-tandem mass spectrometer