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  Indian J Med Microbiol
 

Figure 3: Apoptosis assessment in PA-1 cells by double staining acridine orange/ethidium bromide staining of PA-1 cells treated with ethanolic extract Excoecaria agallocha for 72 h and viewed under the fluorescence microscope (×200). (a) Control untreated viable cells with normal green nucleus (b) 60.5 μg/ml extract treated cells showing early and late apoptotic cells and necrotic cells (c) 100 μg/ml extract treated cells showing late apoptotic cells and necrotic cells (d) doxorubicin-treated cells showing the late apoptotic cells and necrotic cells

Figure 3: Apoptosis assessment in PA-1 cells by double staining acridine orange/ethidium bromide staining of PA-1 cells treated with ethanolic extract Excoecaria agallocha for 72 h and viewed under the fluorescence microscope (×200). (a) Control untreated viable cells with normal green nucleus (b) 60.5 μg/ml extract treated cells showing early and late apoptotic cells and necrotic cells (c) 100 μg/ml extract treated cells showing late apoptotic cells and necrotic cells (d) doxorubicin-treated cells showing the late apoptotic cells and necrotic cells