Figure 4: Kalanchoe blossfeldiana extract induced necrotic changes in the nuclei of human cervical carcinoma cells. The nuclei were stained with Hoechst 33342 dye after treating the cells with dimethyl sulfoxide (0.5%, control) (a) and Kalanchoe blossfeldiana extract at concentrations of 20.0 (b), 50.0 (c) and 100.0 μg/mL (d). The cells incubated with the extract showed dispersed chromatin fragments in comparison to the control sample (dimethyl sulfoxide). Arrows represent nuclear changes in the cells