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Figure 3: Human cervical carcinoma cells processed with Annexin-V-Fluos Staining Kit after treatment with Kalanchoe blossfeldiana extract. The cells were stained with annexin-V-fluorescein (green) and propidium iodide (red) after treating the cells with dimethyl sulfoxide (0.5%, control) (a) and with Kalanchoe blossfeldiana extract at concentrations of 20.0 (b), 50.0 (c) and 100.0 μg/mL (d). Arrows indicate the necrotic cells (white arrows; high annexin and high propidium iodide staining) and apoptotic cells (yellow arrows; high annexin staining) |
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