Close
  Indian J Med Microbiol
 

Figure 3: The assessment of cell death and motility of 1, vehicle dimethyl sulfoxide and positive control staurosporine (25 nM) or camptothecin (1 mM), (a and b) prostate cancer 3 cells (5 × 104) after treatment, were fixed, stained with nuclear DAPI reagent. Apoptotic nuclei were characterized and photographed under a fluorescence microscope (×100), and data were generated from three independent experiments. (c and d) Cell cycle analysis to determine cell cycle phase distribution, (e) wound healing assay (0.5 × 105 cells/well) to assess the degree of wound healing after compound 1 treatment, (f) Colony formation assay against prostate cancer 3 cells (1 × 103 cells/well), in presence of compound 1. After 7 days, the cells were stained with crystal violet. The number of stained cells per colony was counted randomly, and images were captured in ×100 under an inverted microscope. Data were calculated from three independent experiments *P < 0.05

Figure 3: The assessment of cell death and motility of 1, vehicle dimethyl sulfoxide and positive control staurosporine (25 nM) or camptothecin (1 mM), (a and b) prostate cancer 3 cells (5 × 10<sup>4</sup>) after treatment, were fixed, stained with nuclear DAPI reagent. Apoptotic nuclei were characterized and photographed under a fluorescence microscope (×100), and data were generated from three independent experiments. (c and d) Cell cycle analysis to determine cell cycle phase distribution, (e) wound healing assay (0.5 × 10<sup>5</sup> cells/well) to assess the degree of wound healing after compound 1 treatment, (f) Colony formation assay against prostate cancer 3 cells (1 × 10<sup>3</sup> cells/well), in presence of compound 1. After 7 days, the cells were stained with crystal violet. The number of stained cells per colony was counted randomly, and images were captured in ×100 under an inverted microscope. Data were calculated from three independent experiments *<i>P</i> < 0.05