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  Indian J Med Microbiol
 

Figure 3: Effect of Lysiphyllum strychnifolium extract on the mRNA expression of lipopolysaccharide-induced inflammatory mediators in RAW 264.7 cells. RAW 264.7 cells were pre-treated with Lysiphyllum strychnifolium for 3–4 h before stimulated by 1 μg/ml lipopolysaccharide for 12 h. The total RNA was extracted from the cells and the mRNA expression of inflammatory mediators was analyzed using specific primers. GAPDH was served as a loading control. The relative cyclooxygenase-II (a), inducible nitric oxide synthase (b), transforming growth factor-β (c), and tumor necrosis factor-α (d) mRNA levels were quantified and expressed as fold increase over control group. Data are shown as the mean ± standard error of the mean of three independent experiments. #P < 0.05 compared to control; *P < 0.05 compared to lipopolysaccharide; L.S. represents Lysiphyllum strychnifolium

Figure 3: Effect of <i>Lysiphyllum strychnifolium</i> extract on the mRNA expression of lipopolysaccharide-induced inflammatory mediators in RAW 264.7 cells. RAW 264.7 cells were pre-treated with <i>Lysiphyllum strychnifolium</i> for 3–4 h before stimulated by 1 μg/ml lipopolysaccharide for 12 h. The total RNA was extracted from the cells and the mRNA expression of inflammatory mediators was analyzed using specific primers. GAPDH was served as a loading control. The relative cyclooxygenase-II (a), inducible nitric oxide synthase (b), transforming growth factor-β (c), and tumor necrosis factor-α (d) mRNA levels were quantified and expressed as fold increase over control group. Data are shown as the mean ± standard error of the mean of three independent experiments. <sup>#</sup><i>P</i> < 0.05 compared to control; <i>*P</i> < 0.05 compared to lipopolysaccharide; L.S. represents <i>Lysiphyllum strychnifolium</i>