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  Indian J Med Microbiol
 

Figure 2: The activation of caspase-8 and caspase-9 by ethanol extract of Citrus unshiu peel in MCF-7 cells. MCF-7 cells were treated with the indicated concentrations of ethanol extract of Citrus unshiu peel for 24 h. (a) The cell lysates were prepared, and equal amounts of cellular proteins were separated on sodium-dodecyl sulfate-polyacrylamide gels and transferred to membranes. The membranes were probed with the indicated antibodies, and the proteins were visualized using an enhanced chemiluminescence detection system. Actin was used as an internal control. Representative images of at least three independent experiments are shown. (b) The activities of caspases were evaluated using caspase colorimetric assay kits. An example of representative results according to each treatment concentration is presented. The data are expressed as the mean ± standard deviation of three independent experiments (*P < 0.05 vs. untreated control)

Figure 2: The activation of caspase-8 and caspase-9 by ethanol extract of <i>Citrus unshiu</i> peel in MCF-7 cells. MCF-7 cells were treated with the indicated concentrations of ethanol extract of <i>Citrus unshiu</i> peel for 24 h. (a) The cell lysates were prepared, and equal amounts of cellular proteins were separated on sodium-dodecyl sulfate-polyacrylamide gels and transferred to membranes. The membranes were probed with the indicated antibodies, and the proteins were visualized using an enhanced chemiluminescence detection system. Actin was used as an internal control. Representative images of at least three independent experiments are shown. (b) The activities of caspases were evaluated using caspase colorimetric assay kits. An example of representative results according to each treatment concentration is presented. The data are expressed as the mean ± standard deviation of three independent experiments (*<i>P</i> < 0.05 vs. untreated control)