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  Indian J Med Microbiol
 

Figure 1: The effect of ethanol extract of Citrus unshiu peel on MCF-7 cell viability. MCF-7 cells were incubated with different concentrations of ethanol extract of Citrus unshiu peel for 24 h. (a) The cell viability was measured by an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay as described in the Materials and Methods section. The data are expressed as the percentage of cell viability, and they represent the mean ± standard deviation of three independent experiments (*P < 0.05 vs. untreated control). (b) The morphological changes of MCF-7 cells treated with ethanol extract of Citrus unshiu peel in various concentrations were observed under a phase-contrast microscope (×200). (c) The percentage of Annexin V-fluorescein isothiocyanate-positive cells cultured under the same conditions in the top (propidium iodide negative) and bottom (propidium iodide positive) of the right quadrant was indicated. Each point represents the means of two independent experiments

Figure 1: The effect of ethanol extract of <i>Citrus unshiu</i> peel on MCF-7 cell viability. MCF-7 cells were incubated with different concentrations of ethanol extract of <i>Citrus unshiu</i> peel for 24 h. (a) The cell viability was measured by an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay as described in the Materials and Methods section. The data are expressed as the percentage of cell viability, and they represent the mean ± standard deviation of three independent experiments (*<i>P</i> < 0.05 vs. untreated control). (b) The morphological changes of MCF-7 cells treated with ethanol extract of <i>Citrus unshiu</i> peel in various concentrations were observed under a phase-contrast microscope (×200). (c) The percentage of Annexin V-fluorescein isothiocyanate-positive cells cultured under the same conditions in the top (propidium iodide negative) and bottom (propidium iodide positive) of the right quadrant was indicated. Each point represents the means of two independent experiments