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  Indian J Med Microbiol
 

Figure 6: Impacts of simvastatin (Sim) on intracellular reactive oxygen species and articulation of NADPH oxidase and heme oxygenase-1 in microglial cells: BV-2 cells pretreated with simvastatin for 30 min, and then, lipopolysaccharide was added and cultured for four hours. After incubation with 10 mM of dichloro-dihydro-fluorescein diacetate, the generation of intracellular reactive oxygen species measured by flow cytometry (a) The mRNA expression of gp91phox (b), p47phox (c), and p22phox (d) was determined by quantitative reverse transcription-polymerase chain reaction. The relative expression value of control group set to 1. Data represent mean ± standard deviation of three independent experiments. Symbol mark indicates significantly differences from lipopolysaccharide alone treatment group (*P < 0.05, **P < 0.01)

Figure 6: Impacts of simvastatin (Sim) on intracellular reactive oxygen species and articulation of NADPH oxidase and heme oxygenase-1 in microglial cells: BV-2 cells pretreated with simvastatin for 30 min, and then, lipopolysaccharide was added and cultured for four hours. After incubation with 10 mM of dichloro-dihydro-fluorescein diacetate, the generation of intracellular reactive oxygen species measured by flow cytometry (a) The mRNA expression of gp91phox (b), p47phox (c), and p22phox (d) was determined by quantitative reverse transcription-polymerase chain reaction. The relative expression value of control group set to 1. Data represent mean ± standard deviation of three independent experiments. Symbol mark indicates significantly differences from lipopolysaccharide alone treatment group (*<i>P</i> < 0.05, **<i>P</i> < 0.01)