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  Indian J Med Microbiol
 

Figure 4: Effect of simvastatin (Sim) on lipopolysaccharide-induced pro-inflammatory genes expression in microglial cells, BV-2 cells pretreated with simvastatin for 30 min, and then lipopolysaccharide was added and cultured for 24 hours. The mRNA levels of (a) tumor necrosis factor-α, (b) inducible nitric oxide synthase, (c) cyclooxygenase-2 and interleukin-1β (d) assessed by quantitative reverse transcription-polymerase chain reaction. The protein levels of inducible nitric oxide synthase and cyclooxygenase-2 evaluated by Western blot (e). The tubulin used as an internal control. Data represent mean ± standard deviation of three independent experiments. Symbol mark indicates significant differences from lipopolysaccharide alone treatment group (*P < 0.05)

Figure 4: Effect of simvastatin (Sim) on lipopolysaccharide-induced pro-inflammatory genes expression in microglial cells, BV-2 cells pretreated with simvastatin for 30 min, and then lipopolysaccharide was added and cultured for 24 hours. The mRNA levels of (a) tumor necrosis factor-α, (b) inducible nitric oxide synthase, (c) cyclooxygenase-2 and interleukin-1β (d) assessed by quantitative reverse transcription-polymerase chain reaction. The protein levels of inducible nitric oxide synthase and cyclooxygenase-2 evaluated by Western blot (e). The tubulin used as an internal control. Data represent mean ± standard deviation of three independent experiments. Symbol mark indicates significant differences from lipopolysaccharide alone treatment group (*<i>P</i> < 0.05)