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  Indian J Med Microbiol
 

Figure 4: (a) The effects of subfractions (0–200 mg/ml) purified from F4 of Nigella sativa hexane extract on the viability of PC12 cells. (b) The effects of nontoxic concentration of subfractions against cytotoxicity induced by MPP+. Incubation of PC12 cells with MPP+ (1.5 mM) induced a decrease in cell viability, while F4d1, F4c4, and F4c5 subfractions had protective effect. The cell viability was determined by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide assay as described in materials and methods. Data are expressed as the mean ± standard error of the mean of three separate experiments. ###P < 0.001 versus control, **P < 0.01, *P < 0.05 MPP+ treated cells. MPP+: 1-methyl-4-phenylpyridinium

Figure 4: (a) The effects of subfractions (0–200 mg/ml) purified from F4 of <i>Nigella sativa</i> hexane extract on the viability of PC12 cells. (b) The effects of nontoxic concentration of subfractions against cytotoxicity induced by MPP<sup>+</sup>. Incubation of PC12 cells with MPP<sup>+</sup> (1.5 mM) induced a decrease in cell viability, while F4d1, F4c4, and F4c5 subfractions had protective effect. The cell viability was determined by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide assay as described in materials and methods. Data are expressed as the mean ± standard error of the mean of three separate experiments. <sup>###</sup><i>P</i> < 0.001 versus control, **<i>P</i> < 0.01, *<i>P</i> < 0.05 MPP<sup>+</sup> treated cells. MPP<sup>+</sup>: 1-methyl-4-phenylpyridinium