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  Indian J Med Microbiol
 

Figure 1: Chromatograms (HPLC-PDA) of (A) isolated compounds from stem bark of E. velutina – 1, hesperetin; 2, abissinin; 3, homohesperetin; 4, rizonic acid; 5, sigmoidin C – and (B) ethanolic extract of E. velutina (EEEV). Conditions: C8 column, mobile phase [(a) ammonium acetate buffer 5 mM, pH = 3.46; (b), acetonitrile], elution gradient, injection volume of 20 μL (microliter), flow rate of 1.0 mL/min and detection: 0–21 min at 286 nm, 21–28 min at 260 nm, 28–30; 7 min at 286 nm, 30; 7–35 min at 267 nm; and 35–45 min at 282 nm.

Figure 1: Chromatograms (HPLC-PDA) of (A) isolated compounds from stem bark of <i>E. velutina</i> – 1, hesperetin; 2, abissinin; 3, homohesperetin; 4, rizonic acid; 5, sigmoidin C – and (B) ethanolic extract of E. velutina (EEEV). Conditions: C8 column, mobile phase [(a) ammonium acetate buffer 5 mM, pH = 3.46; (b), acetonitrile], elution gradient, injection volume of 20 μL (microliter), flow rate of 1.0 mL/min and detection: 0–21 min at 286 nm, 21–28 min at 260 nm, 28–30; 7 min at 286 nm, 30; 7–35 min at 267 nm; and 35–45 min at 282 nm.