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  Indian J Med Microbiol
 

Figure 4: Effect of thymoquinone on cyclooxygenase-2 and caspase 3 expression in methotrexate-treated rat intestine. Localization of cyclooxygenase-2 (left panel) and caspase 3 (right panel) was assessed by immunohistochemical staining (×200) of: (a and f) Control, (b and g) thymoquinone-treated, (c and h) methotrexate-treated, and (d and i) combined methotrexate/thymoquinone-treated groups. Arrows point at areas of up-regulated cellular expression. (e and j) represent semi-quantitative analysis of the results for cyclooxygenase-2 and caspase 3 expression, respectively. Values represent means ± standard error of the mean of percent of immunopositive cells for the respective marker per field of each animal, 3 fields/animal. Significant difference is reported when P < 0.05. aSignificant difference compared with control and bsignificant difference compared with methotrexate group

Figure 4: Effect of thymoquinone on cyclooxygenase-2 and caspase 3 expression in methotrexate-treated rat intestine. Localization of cyclooxygenase-2 (left panel) and caspase 3 (right panel) was assessed by immunohistochemical staining (×200) of: (a and f) Control, (b and g) thymoquinone-treated, (c and h) methotrexate-treated, and (d and i) combined methotrexate/thymoquinone-treated groups. Arrows point at areas of up-regulated cellular expression. (e and j) represent semi-quantitative analysis of the results for cyclooxygenase-2 and caspase 3 expression, respectively. Values represent means ± standard error of the mean of percent of immunopositive cells for the respective marker per field of each animal, 3 fields/animal. Significant difference is reported when <i>P</i> < 0.05. <sup>a</sup>Significant difference compared with control and <sup>b</sup>significant difference compared with methotrexate group