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  Indian J Med Microbiol
 

Figure 2: (a) Dose-dependent fibrinogenolytic activity of Maclura spinosa latex crude extract. Different protein concentrations of Maclura spinosa latex ranging from 1 to 10 µg were incubated with 50 μg of human fibrinogen at 37°C for 3 h in the presence of 10 mM Tris-HCl buffer (pH 7.6). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (10%) was performed to visualize the fibrinogen degradation pattern. (A) Control (B) 1 μg (C) 2.5 μg (D) 5 μg (E) 7.5 μg (F) 10 μg of Maclura spinosa latex. (b) Densitometric analysis of γ bands obtained in electrophoretogram of the dose-dependent fibrinogenolytic assay. Band percentage versus concentration of M. spinosa latex was done. All the values represent mean ± standard deviation (n = 3)

Figure 2: (a) Dose-dependent fibrinogenolytic activity of <i>Maclura spinosa</i> latex crude extract. Different protein concentrations of <i>Maclura spinosa</i> latex ranging from 1 to 10 µg were incubated with 50 μg of human fibrinogen at 37°C for 3 h in the presence of 10 mM Tris-HCl buffer (pH 7.6). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (10%) was performed to visualize the fibrinogen degradation pattern. (A) Control (B) 1 μg (C) 2.5 μg (D) 5 μg (E) 7.5 μg (F) 10 μg of <i>Maclura spinosa</i> latex. (b) Densitometric analysis of γ bands obtained in electrophoretogram of the dose-dependent fibrinogenolytic assay. Band percentage versus concentration of <i>M. spinosa</i> latex was done. All the values represent mean ± standard deviation (<i>n</i> = 3)