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  Indian J Med Microbiol
 

Figure 4: Inhibition of the human fibrinogenolytic activity of Maclura spinosa latex crude by specific protease inhibitors. Maclura spinosa latex 5 μg was preincubated with and without specific protease inhibitors for 15 min in the presence of 10 mM Tris-HCl buffer (pH 7.6) and the reaction was initiated by adding 50 μg of fibrinogen. After 3 h, the reaction was terminated by adding a denaturing buffer. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (10%) was performed to visualize the inhibition pattern of fibrinogen degradation. (A) Fifty micrograms of fibrinogen, (B) 50 μg fibrinogen + 5 μg Maclura spinosa latex, (C) 50 μg fibrinogen + 5 μg Maclura spinosa latex + 100 μM iodoacetic acid, (D) 50 μg fibrinogen + 5 μg Maclura spinosa latex + 5 mM phenylmethylsulfonyl fluoride, (E) 50 μg fibrinogen + 5 μg Maclura spinosa latex + 5 mM ethylenediaminetetraacetic acid, (F) 50 μg fibrinogen + 5 μg Maclura spinosa latex + 5 mM ethylene glycol tetraacetic acid

Figure 4: Inhibition of the human fibrinogenolytic activity of <i>Maclura spinosa</i> latex crude by specific protease inhibitors. <i>Maclura spinosa</i> latex 5 μg was preincubated with and without specific protease inhibitors for 15 min in the presence of 10 mM Tris-HCl buffer (pH 7.6) and the reaction was initiated by adding 50 μg of fibrinogen. After 3 h, the reaction was terminated by adding a denaturing buffer. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (10%) was performed to visualize the inhibition pattern of fibrinogen degradation. (A) Fifty micrograms of fibrinogen, (B) 50 μg fibrinogen + 5 μg <i>Maclura spinosa</i> latex, (C) 50 μg fibrinogen + 5 μg <i>Maclura spinosa</i> latex + 100 μM iodoacetic acid, (D) 50 μg fibrinogen + 5 μg <i>Maclura spinosa</i> latex + 5 mM phenylmethylsulfonyl fluoride, (E) 50 μg fibrinogen + 5 μg <i>Maclura spinosa</i> latex + 5 mM ethylenediaminetetraacetic acid, (F) 50 μg fibrinogen + 5 μg <i>Maclura spinosa</i> latex + 5 mM ethylene glycol tetraacetic acid