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  Indian J Med Microbiol
 

Figure 3: (a) Time-dependent fibrinogenolytic activity of Maclura spinosa latex crude extract. Maclura spinosa latex (5 μg) was incubated with 50 μg of human fibrinogen at 37°C for different incubation time ranging from 0 to 3 h in the presence of 10 mM Tris-HCl buffer (pH 7.6). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (10%) was performed to visualize the fibrinogen degradation pattern. (A) Control (B) 15 min (C) 30 min (D) 1 h (E) 2 h (F) 3 h. (b) Densitometric analysis of γ bands obtained in electrophoretogram of time-dependent fibrinogenolytic assay. Band percentage versus time (min) was done. All the values represent mean ± standard deviation (n = 3)

Figure 3: (a) Time-dependent fibrinogenolytic activity of <i>Maclura spinosa</i> latex crude extract. <i>Maclura spinosa</i> latex (5 μg) was incubated with 50 μg of human fibrinogen at 37°C for different incubation time ranging from 0 to 3 h in the presence of 10 mM Tris-HCl buffer (pH 7.6). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (10%) was performed to visualize the fibrinogen degradation pattern. (A) Control (B) 15 min (C) 30 min (D) 1 h (E) 2 h (F) 3 h. (b) Densitometric analysis of γ bands obtained in electrophoretogram of time-dependent fibrinogenolytic assay. Band percentage versus time (min) was done. All the values represent mean ± standard deviation (<i>n</i> = 3)