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Figure 2: Cultures were treated with the methanolic extract of L. japonica and 5 mM Fura-2 AM 1 hour before exposure to 100 μM glutamate. The change of [Ca2+]i was measured 3 hour after glutamate exposure. The values shown are means ± Standard deviation of three experiments (3-4 cultures per experiment). *P<0.05, **P<0.01 vs. Glutamate-injured cells (Analysis of Variance [ANOVA] and Tukey) |
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