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2012| October-December | Volume 8 | Issue 32
Online since
November 22, 2012
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ORIGINAL ARTICLES
Determination of quercetin, plumbagin and total flavonoids in
Drosera peltata Smith var. glabrata
Y.Z.Ruan
Yu He, Zhimin He, Feng He, Haitong Wan
October-December 2012, 8(32):263-267
DOI
:10.4103/0973-1296.103649
PMID
:24082628
Background:
Drosera peltata Smith var. glabrata
Y.Z.Ruan, a kind of wild carnivorous plants in the family Droseraceae, has been used for the treatment of rheumatism and bruises in Chinese folk. None of compounds in this herb has been quantified in the previous studies.
Objective:
To develop a validated and reliable HPLC method for the simultaneous determination of two bioactive constituents - quercetin and plumbagin, and establish a simple UV spectrophotometry method for the analysis of total flavonoids content.
Materials and Methods:
Chromatographic separation was performed by using a HPLC system consisting of an Agilent Eclipse XDB C
18
column and a gradient elution system of acetonitrile and water (containing 0.1% phosphoric acid, V/V) within 20 minutes. Comparing with quercetin complex with Al(NO
3
)
3
, the total flavonoids were determined by UV spectrophotometry at 269 nm.
Results:
Both methods were validated for linearity (r
2
≥0.9994 for quercetin and plumbagin in the HPLC method, r
2
=0.9994 for quercetin in the UV spectrophotometry method), precision (The within-day and between-day variability was less than 0.738% and 1.64% for quercetin and plumbagin in the HPLC method, and was less than 1.67% for quercetin in the UV spectrophotometry method.) and recovery (The recoveries of the HPLC method were 96.7-100.4% and 97.4-100.4% for quercetin and plumbagin, respectively, and the recovery of the UV spectrophotometry method was 96.7-99.6% for quercetin.)
Conclusion:
The proposed methods are simple and accurate, and could be practiced to rapidly determine quercetin, plumbagin and total flavonoids in the herbal drug, which provide effective approaches for quality control.
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6,425
345
8
Antipsoriatic activity and cytotoxicity of ethanolic extract of
Nigella sativa
seeds
Lalitha Priyanka Dwarampudi, Dhanabal Palaniswamy, Muruganantham Nithyanantham, PS Raghu
October-December 2012, 8(32):268-272
DOI
:10.4103/0973-1296.103650
PMID
:24082629
Background:
Nigella sativa
Linn (Ranunculaceae) is popularly known as black cumin with a wide spectrum of pharmacological activities including anti-inflammatory, antibacterial, antifungal and antihelmenthic. The seeds are externally applied for eruptions of skin. The seeds are used traditionally for psoriasis tropicus with general pain and eruption of patches.
Objective:
The ethanolic extract of
Nigella sativa
seeds were evaluated for antipsoriatic activity.
Materials and Methods:
The screening of antipsoriatic activity of 95% of ethanolic extract of
Nigella sativa
seeds by using mouse tail model for psoriasis and
in vitro
antipsoriatic activity was carried out by SRB Assay using HaCaT human keratinocyte cell lines.
Results:
The ethanolic extract of
Nigella sativa
seeds extract produced a significant epidermal differentiation, from its degree of orthokeratosis (71.36±2.64) when compared to the negative control (17.30±4.09%). This was equivalent to the effect of the standard positive control, tazarotene (0.1%) gel, which showed a (90.03±2.00%) degree of orthokeratosis. The 95% ethanolic extract of
Nigella sativa
shown IC50 239 μg/ml, with good antiproliferant activity compared to Asiaticoside as positive control which showed potent activity with IC50 value of 20.13 μg/ml.
Conclusion:
The ethanolic extract of
Nigella sativa
seeds also showed increase in relative epidermal thickness when compared to control group by confirming its traditional use in psoriasis treatment.
[ABSTRACT]
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5,889
228
18
In vivo
pharmacokinetic comparisons of ferulic acid and puerarin after oral administration of monomer, medicinal substance aqueous extract and Nao-De-Sheng to rats
Zhen Ouyang, Ming Zhao, Jianming Tang, Lulin Pan
October-December 2012, 8(32):256-262
DOI
:10.4103/0973-1296.103648
PMID
:24082627
Background:
Nao-De-Sheng decoction (NDS), a traditional Chinese medicine (TCM) prescription containing
Radix puerariae lobatae
,
Floscarthami, Radix et Rhizoma Notoginseng
,
Rhizoma chuanxiong
and
Fructus crataegi
, is effective in the treatment of cerebral arteriosclerosis, ischemic cerebral stroke and apoplexy linger effect. Ferulic acid and puerarin are the main absorbed effective ingredients of NDS.
Objective:
To assess the affection of other components in medical material and compound recipe compatibility on the pharmacokinetics of ferulaic acid and puerarin, of ferulic acid from the monomer
Rhizoma chuanxiong
aqueous extract and NDS were studied. And pharmacokinetics comparisons of puerarin from the monomer
Radix puerariae
extract and NDS decoction were investigated simultaneously.
Materials and Methods:
At respective different time points after oral administration of the monomer, medicinal substance aqueous extract and NDS at the same dose in rats, plasma concentrations of ferulic acid and puerarin in rats were determined by RP-HPLC, and the main pharmacokinetic parameters were estimated with 3P97 software.
Results:
The plasma concentration-time curves of ferulaic acid and puerarin were both best fitted with a two-compartment model. AUC
0−
t
, AUC
0
→
∞
,
T
max
, and
C
max
of ferulic acid in the monomer and NDS decoction were increased significantly (
P
< 0.05) compared with that in
Rhizoma chuanxiong
aqueous extract. And statistically significant increase (
P
< 0.05) in pharmacokinetic parameters of puerarin including AUC
0−
t
, AUC
0
→
∞
, CL,
T
max
and
C
max
were obtained after oral administration of puerarin monomer compared with
Radix puerariae
extract. Although the changes of AUC
0−
t
, AUC
0
→
∞
and CL had no statistically significant,
C
max
of puerarin in NDS was increased remarkably (
P
< 0.05) compared with that in single puerarin.
Conclusions:
Some ingredients of
Rhizoma chuanxiong
and
Radix puerariae
may be suggested to remarkably influence plasma concentrations of ferulaic acid and puerarin. Some ingredients in NDS may increase dissolution and absorption of ferulaic acid and puerarin, delay elimination, and subsequently enhance bioavailability of ferulaic acid and puerarin in rats after compatibility.
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4,552
151
10
Comparison of the antiproliferative activity of crude ethanol extracts of nine
salvia
species grown in Jordan against breast cancer cell line models
Rana Abu-Dahab, Fatma Afifi, Violet Kasabri, Lara Majdalawi, Randa Naffa
October-December 2012, 8(32):319-324
DOI
:10.4103/0973-1296.103664
PMID
:24082637
Background:
The antiproliferative activity of
Salvia
species grown in Jordan has not been fully evaluated yet. The aim of this work was to study the antiproliferative activity of crude ethanol extracts from nine
Salvia
species grown in Jordan against a panel of breast cancer cell lines.
Material and Methods:
Cytotoxic activity was evaluated in human tumor models of breast cancer; MCF-7, T47D, ZR-75-1, and BT 474 by the sulforhodamine B assay. In addition, the extracts were evaluated using a non-transformed cell line (Vero) and normal fibroblast cells in order to demonstrate their selectivity and safety.
Results:
From the nice ethanol extracts under investigation, those of
S. dominica
and
S. fruticosa
showed an inhibitory concentration of 50% of cells (IC
50
) in concentrations less than 30μg/mL against the four cell lines under investigation.
S. syriaca
and
S. hormium
showed an IC
50
below 30μg/ml for two out of the four cell lines.
S. fruticosa,
S. hormium
and
S. syriaca
showed selectivity in their antiproliferative activity against estrogen receptor positive cell lines with minimal toxicity against normal human periodontal fibroblasts. Phytochemical screening using thin layer chromatography indicated the presence of terpenoids, flavonoids and coumarins in all examined extracts.
Conclusion:
Three of the plant extracts under investigation exhibited antiproliferative activity against breast cancer cells and were shown to be safe and selective. These could be considered as a potential source for novel anticancer therapy.
[ABSTRACT]
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4,557
121
16
Simultaneous determination of five marker compounds in Xuanfu Daizhe Tang by high-performance liquid chromatography coupled with diode array detection for quality control
Kunming Qin, Bin Wang, Hao Cai, Weidong Li, Zhongqing Yao, Xingde Zhang, Tulin Lu, Baochang Cai
October-December 2012, 8(32):250-255
DOI
:10.4103/0973-1296.103647
PMID
:24082626
Background:
Xuanfu Daizhe Tang (XDT) is a classical traditional Chinese medicinal prescription that has been widely used for treating digestive system illnesses for hundreds of years.
Materials and Methods:
In this study, a simple and sensitive high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) method was established for the simultaneous determination of five marker compounds in XDT including chlorogenic acid, glycyrrhizic acid, ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Re, for quality control of this well-known traditional Chinese medicine (TCM).
Results:
These compounds were separated in less than 130 min using a YMC C18 column with a gradient elution system of acetonitrile and 0.1% phosphoric acid water solution at a flow rate of 1 ml/min. All calibration curves of standard components showed good linearity with R
2
>0.9991. Limit of detection and limit of quantification varied from 0.11 to 4.3 μg/ml and 0.20 to 11.6 μg/ml, respectively. The relative standard deviations (RSDs) of the intra-day and inter-day experiments were less than 4.72 and 5.48%, respectively. The accuracy of recovery test ranged from 95.0 to 105.0% with RSD values 1.28- 4.32%.
Conclusion:
The validated method is simple, reliable, and successfully applied to determine the contents of the selected compounds in XDT for quality control.
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[PubMed]
4,170
224
5
Zinc administration modulates radiation-induced oxidative injury in lens of rat
Seyithan Taysi, Seydi Okumus, Mehmet Akyuz, Naim Uzun, Adnan Aksoy, Elif Demir, Mustafa Orkmez, Mehmet Tarakcioglu, Mustafa Adli
October-December 2012, 8(32):245-249
DOI
:10.4103/0973-1296.103646
PMID
:24082625
Background
: The aim of this study was to evaluate the antioxidant role of zinc (Zn) against radiation-induced cataract in the rat lens after total cranial irradiation with a single 5 Gray (Gy) dose of gamma irradiation.
Materials and Methods
: Twenty-one Sprague-Dawley rats were used for the experiment. The control group did not receive Zn or irradiation but received 1-ml saline orally plus sham-irradiation. The irradiation (IR) group received 5 Gy gamma irradiation to the total cranium as a single dose plus 0.1 ml physiological saline intraperitoneally. The IR plus Zn group received irradiation to total cranium plus 10 mg/kg/day Zn intraperitoneally. Biochemical parameters measured in rat lenses were carried out using spectrophotometric techniques.
Results:
Lens total (enzymatic plus non-enzymatic) superoxide scavenger activity (TSSA), glutathione reductase (GRD), and glutathione-S-transferase (GST) activities significantly increased in the IR plus Zn groups when compared with the IR group. However, TSSA, GRD and GST activities were significantly lower in the IR group when compared with the control group. Lens non-enzymatic superoxide scavenger activity (NSSA) in the IR plus Zn group was significantly increased compared to that of the IR group. Lens xanthine oxidase (XO) activity in the IR group significantly increased compared to that of both the control and IR plus Zn groups.
Conclusion
: Zn has clear antioxidant properties and prevented oxidative stress by scavenging free radicals generated by ionizing radiation in rat lenses.
[ABSTRACT]
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[PubMed]
3,991
274
10
Quantitative determination of total and individual flavonoids in stems and leaves of
Buddleja davidii
and
Buddleja albiflora
Cheng Ying, Dingrong Wan
October-December 2012, 8(32):273-279
DOI
:10.4103/0973-1296.103651
PMID
:24082630
Background:
Buddleja davidii
and
B. albiflor
a are two different original plants of the famous crude medicine "Diaoyangchen."
Materials and Methods:
An ultraviolet-visible spectrophotometric method and a HPLC method were used for the determination of total and individual flavonoids (luteolin and apigenin) contents from their stems and leaves for the first time.
Results:
From the comparative evaluation, remarkable differences in flavonoids contents were observed between different origins and different parts of the samples. And content of specific flavonoid did not correspond to the total flavonoids contents in
Buddleja davidii
and
Buddleja albiflora
.
Conclusion:
With a better accuracy and precision, the methods had been proved simple, rapid, and reliable for quantitative determination of the total flavonoids and luteolin and apigenin in the two phytomedicines. Furthermore, our present study will pave the way of guidelines for the differentiation and standardization and exploitation of individual parts of this herb material.
[ABSTRACT]
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4,059
131
8
Study on chemical fingerprinting of crude and processed
Atractylodes macrocephala
from different locations in Zhejiang province by reversed-phase high-performance liquid chromatography coupled with hierarchical cluster analysis
Hao Cai, Zhiwei Xu, Sucai Luo, Wenwen Zhang, Gang Cao, Xiao Liu, Yajing Lou, Xiaoqing Ma, Kunming Qin, Baochang Cai
October-December 2012, 8(32):300-307
DOI
:10.4103/0973-1296.103659
PMID
:24082634
Background:
In China,
Atractylodes macrocephala
is mainly distributed in Zhejiang province. It is, therefore, desirable to determine a reliable and accurate methodology to differentiate the samples collected from Zhejiang province. Although some studies on the fingerprints of
Atractylodes macrocephala
using HPLC have been published, none of them compared the processed product of
Atractylodes macrocephala
from different areas of Zhejiang province.
Materials and Methods:
Reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with hierarchical cluster analysis was employed in the fingerprint analysis of
Atractylodes macrocephala
from Zhejiang province, China. The LC assay was performed on a reversed-phase C
18
column with linear gradient elution using water and acetonitrile. The LC data showed considerable variation of chemical constituents among
Atractylodes macrocephala
populations.
Results:
21 and 22 characteristic peaks in the 14 grants of
Atractylodes macrocephala
and its processed product were determined in samples from different habitats of Zhejiang province, respectively. Their chromatographic patterns were generally consistent although their contents of chemical compositions were greatly different. The results of hierarchical cluster analysis showed that the samples could be divided into four groups; it was able to select excellent resources from the groups.
Conclusion:
This was the first report of hierarchical cluster analysis of crude and processed
Atractylodes macrocephala
according to their chemical fingerprints and could be applied to the intrinsic quality control of crude and processed
Atractylodes macrocephala
. The processing technique of
Atractylodes macrocephala
through the pilot-scale experiment was first studied and is simple and suitable for prepared
yin pian
of
Atractylodes macrocephala
industrial manufacture.
[ABSTRACT]
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[PubMed]
3,776
97
13
Development of a rapid resolution liquid chromatography-diode array detector method for the determination of three compounds in
Ziziphora clinopodioides
Lam from different origins of Xinjiang
Shuge Tian, Qian Yu, Dongdong Wang, Halmuart Upur
October-December 2012, 8(32):280-284
DOI
:10.4103/0973-1296.103653
PMID
:24082631
Context:
As a traditional Uygur medicinal plant,
Z. clinopodioides
Lam has various uses in Xinjiang.
Aims:
A reversed-phase rapid resolution liquid chromatography (RP-RRLC) method with diode array detector (DAD) was developed for simultaneous determination of diosmin, linarin, and pulegone from
Ziziphora clinopodioides
Lam, a widely used in traditional Uygur medicine for treating heart disease, high blood pressure, and other diseases.
Settings and Design:
Compounds were separated on a XDB-C18 reversed-phase analytical column (50 mm × 4.6 mm, 1.8 μm) with gradient elution using methanol and 1% aqueous acetic acid (v/v) at 0.9 mL/min. he detection wavelength was set at 270 nm.
Materials and Methods:
Ziziphora clinopodioides
Lam. were collected from ten different origins in Xinjiang, including the Ban fang ditch, Tuoli, the Altay mountains, Terks, Xiata Road, Zhaosu Highway, Guozigou, Fukang, Jimsar, Wulabo.
Statistical Analysis Used:
The intra-day and inter-day precisions of all three compounds were less than 0.89% and the average recoveries ranged from 97.4 to 104.1%. There were highly significant linear correlations between component concentrations and specific chromatographic peak areas (R2 > 0.999).
Results:
The proposed method was successfully applied to determine the levels of three active components in
Z. clinopodioides
Lam. samples from different locations in Xinjiang.
Conclusions:
The proposed method is simple, consistent, accurate, and could be utilized as a quality control method for
Z. clinopodioides
Lam.
[ABSTRACT]
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[PubMed]
3,757
74
4
Effect of the hexane extract of
Piper auritum
on insulin release from β-cell and oxidative stress in streptozotocin-induced diabetic rat
Rosa Martha Perez Gutierrez
October-December 2012, 8(32):308-313
DOI
:10.4103/0973-1296.103661
PMID
:24082635
Background:
The large-leafed perennial plant
Piper auritum
known as Hoja Santa, is used for its leaves that because of their spicy aromatic scent and flavor have an important presence in Mexican cuisine, and in many regions, this plant is known for its therapeutic properties.
Materials and Methods:
In the present study, we investigated the effect of hexane, chloroform and methanol extracts from
Piper auritum
on cell culture system and the effect in streptozotocin-induced type 1 diabetic rats treated by 28 days on the physiological, metabolic parameters and oxidative stress.
Results:
The hexane extract of
P. auritum
(HS) treatment significantly reduced the intake of both food, water and body weight loss as well as levels of blood glucose, serum cholesterol, triglycerides and increase HDL-cholesterol. After 4-week administration of HS antioxidant enzyme as SOD, CAT, GSH, GPx in pancreas were determined. These enzyme increased significantly compared with those of the diabetic rats control and normal animals. For all estimated, the results of HS treated groups leading to a restoration of the defense mechanism. The treatment also improves pancreatic TBARS-reactive substance level and serum NO and iNOS. To determine the insulin releasing activity, after extract treatment the serum and pancreatic sections were processed for examination of insulin-releasing activity using an immunocytochemistry kit. The results showed that administration of the hexane extract (200 and 400 mg/kg) exhibited a significant increase in serum and pancreas tissue insulin. Administration of streptozotocin decreased the insulin secretory activity in comparison with intact rats, but treatment with the HS extract increased significantly the activity of the beta cells in comparison with the diabetic control rats. The extract decreased serum glucose in streptozotocin-induced diabetic rats and increased insulin release from the beta cells of the pancreas. In cultured RIN-5F cells, we examined whether hexane extract of
P. auritum
would protect the pancreas-derived β-cells from oxidative stress. Moreover, HS could protect pancreatic β-cells from advanced glycation end products-induced oxidative stress.
Conclusion:
From these results, HS is suggested to show anti-diabetic effect by stimulating insulin-dependent and by protecting pancreatic β-cells from advanced glycation end products-induced oxidative stress.
[ABSTRACT]
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3,667
88
6
Effect and evaluation of antihyperlipidemic activity guided isolated fraction from total methanol extract of
Salvadora oleoides (Decne.)
in Triton WR-1339 Induced hyperlipidemic rats
Deepak Kumar, Versha Parcha, Alok Maithani, Ishan Dhulia
October-December 2012, 8(32):314-318
DOI
:10.4103/0973-1296.103663
PMID
:24082636
Background:
Hyperlipidemia is implicated as the cause for coronary heart diseases. Though varieties of synthetic drugs are used in the treatment, still the searches are on for better medicaments especially from the plant kingdom. Many medicinal plants have been studied in this context but most of them are seasonal or have restricted availability. One such weed, available throughout the year is
Salvadora oleoides
(decne.).
Materials and Methods:
Column chromatographic fractionation of the butanol fraction of leaves of
Salvadora oleoides
(decne.) yielded four fractions (fraction A-D). All sub-fractions were tested for their anti-hyperlipidemic activity. Fractions were administered at a dose of 65 mg/kg (oral) to the Triton WR-1339 induced hyperlipidemic rats.
Results:
Sub-fraction D showed maximum significant reduction (
P
<0.05) among four sub-fractions in comparison with standard drug fenofibrate.
Conclusion:
Further studies on the isolated fractions and constituents are needed to isolate compound responsible for activity and elucidate the mechanism by which
Salvadora oleoides
(decne.) exerts protective effects against hyperlipidemia.
[ABSTRACT]
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3,417
115
4
Anticonvulsant and anxiolytic activity of the peptide fraction isolated from the venom of the social wasp
Polybia paulista
Lucianna Lopes do Couto, Lilian Carneiro dos Anjos, Maíra de Azevedo Feitosa Araujo, Cecília Alves Mourão, Carlos Alberto Schwartz, Luzitano Brandão Ferreira, Márcia Renata Mortari
October-December 2012, 8(32):292-299
DOI
:10.4103/0973-1296.103657
PMID
:24082633
Background:
Arthropod venoms have attracted interest because they represent a source of neuroactive compounds that can be useful tools in neuroscience and pharmacological investigations.
Objective:
The purpose of the present work was to evaluate the anticonvulsant, anxiolytic, and behavioral effects of the peptide fraction separated from venom of the social wasp.
Materials and Methods:
The low- molecular-weight compounds of the venom were separated by ultrafiltration and the bioassays were performed to test anticonvulsant and anxiolytic effects, as well as alterations in the spontaneous behavior of the animals.
Results:
Intracerebroventricular injections of the compounds induced dose-dependent anticonvulsant effects and a potent anxiolytic activity. Regarding behavioral effects, no significant differences were observed in relation to the saline control group.
Conclusion:
The low-molecular-weight compounds of the venom of
Polybia paulista
include neuroactive peptides that can be used as pharmacological resources for anticonvulsant and anxiolytic drug research.
[ABSTRACT]
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[PubMed]
3,265
70
8
Modulatory effects of
Crataeva nurvala
bark against testosterone and N-methyl-N-nitrosourea-induced oxidative damage in prostate of male albino rats
Dugganaboyana Guru Kumar, Purandekkattil Deepa, Muthaiyan A Rathi, Periasamy Meenakshi, Velliyur K Gopalakrishnan
October-December 2012, 8(32):285-291
DOI
:10.4103/0973-1296.103654
PMID
:24082632
Background:
Antioxidant properties of
Crataeva nurvala
bark contains a variety of the bioactive phytochemical constituents in medicinal plants which include flavonoids, phenolic compounds, tannins, anthracene derivatives, and essential oils. Components from
Crataeva nurvala
bark have been accounted to play an important role in scavenging free radicals generated by mutagens and carcinogens. Androgens are the key factors in either the initiation or progression of prostate cancer by inducing oxidative stress. In the present set of investigations, the antioxidative potential of
Crataeva nurvala
bark extract against androgen-mediated oxidative stress in male Wistar rats has been studied.
Materials and Methods:
Oxidative damage in prostate was induced in rats by the injection of testosterone (100 mg/kg body weight [bw]) for 3 days followed by injection of chemical carcinogen
N
-Methyl
N
-Nitroso Urea (50 mg/kg bw) for 1 week. The oxidative damage in prostate-induced rats were treated with the ethanolic extract of
Crataeva nurvala
bark (150 mg/kg bw) and testosterone injection (2 mg/ kg bw) was also continued through the experimental period of 4 months. The prostate tissue was dissected out for biochemical analysis of lipid peroxidation and enzymic-antioxidants viz. catalase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase, and glutathione reductase; the non-enzymic antioxidants viz. reduced glutathione, and Vitamin C.
Results:
The results revealed tha
t test
osterone administration induced the oxidative stress in rat prostate; however, in drug (150 mg/kg bw) supplemented groups, a significant protective effect of
Crataeva nurvala
bark agains
t test
osterone-induced oxidative injury was recorded.
Conclusion:
Hence, the study reveals that constituents present in
Crataeva nurvala
bark impart protection against androgen-induced oxidative injury in prostate.
[ABSTRACT]
[FULL TEXT]
[PDF]
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[EPub]
[CITATIONS]
[PubMed]
3,233
77
1
Life span effects of
Hypericum perforatum
extracts on
Caenorhabditis elegans
under heat stress
Hasan Kiliçgün, Gülden Göksen
October-December 2012, 8(32):325-328
DOI
:10.4103/0973-1296.103666
PMID
:24082638
Background:
The beneficial effects of antioxidants in plants are mainly extrapolated from
in vitro
studies or short-term dietary supplementation studies. Due to cost and duration, relatively little is known about whether dietary antioxidants are beneficial in whole animals' life span or not.
Materials and Methods:
To address this question, under heat stress (35°C),
Hypericum perforatum
was extracted with petroleum ether and the nematodes
Caenorhabditis elegans
exposed to three different extract concentrations (1mg/mL, 0.1mg/mL, 0.01mg/mL) of
H. perforatum
.
Results:
We report that
Hypericum perforatum
extracts did not increase life span and slow aging related increase in
C. elegans
. Moreover, one fraction (1mg/mL) increased declines of C. elegans life span and thermotolerance.
Conclusion:
Given this mounting evidence for life span role of
H. perforatum
in the presence of heat stress in vivo, the question whether
H. perforatum
acts as a prooxidant or an antioxidant in vivo under heat stress arises.
[ABSTRACT]
[FULL TEXT]
[PDF]
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[EPub]
[CITATIONS]
[PubMed]
1,901
21
3
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