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Table of Contents
April-June 2017
Volume 13 | Issue 50 (Supplement)
Page Nos. 164-384
Online since Tuesday, July 11, 2017
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ORIGINAL ARTICLES
Chemopreventive and antioxidant effect of polyphenol free
Spirulina maxima
and its hydrolyzed protein content: Investigation on azoxymethane treated mice
p. 164
Nikte Y Martinez-Palma, Gloria Dávila-Ortiz, Cristian Jiménez-Martínez, Eduardo Madrigal-Bujaidar, Isela Álvarez-González
DOI
:10.4103/0973-1296.210197
PMID
:28808375
Background:
Spirulina maxima
(Sm) is known to have nutritive value as well as a number of potentially useful biomedical properties.
Objectives:
The initial purpose of this report was to evaluate the inhibitory effect of the alga (without its polyphenol content), on the induction of azoxymethane (AOM)-induced colon aberrant crypts (AC) in mouse. Besides, we hydrolyzed the protein content of such mixture. Our second aim was to determine the inhibitory potential of this last plant mixture on the AOM-induced colon AC in mouse. Moreover, we also determined the effect of the two indicated Sm samples on the oxidative damage caused by AOM in the colon and liver of treated mice.
Materials and Methods:
The experiment lasted 5 weeks. At the end, we registered the level of AC, nitric oxide, and the lipid and protein oxidation.
Results:
Our results showed the following: (1) the carcinogen increased more than 18 times the amount of the AC found in the control group. (2) On the contrary, the two tested mixtures of Sm produced a significant reduction over this damage (about 45%). (3) The two tested Sm mixtures were generally able to reduce the oxidative stress markers although with variable effects which go from 59% to 100% with respect to the control mice.
Conclusion:
Therefore, the present report established that the tested Sm fractions have mouse colon anticarcinogenic potential, partially related with their antioxidant capacity. Our report also suggested the need to further evaluate specific Sm chemicals as chemopreventive agents.
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Isolation of abscisic acid from Korean acacia honey with anti-
Helicobacter pylori
activity
p. 170
SeGun Kim, InPyo Hong, SoonOk Woo, HyeRi Jang, SokCheon Pak, SangMi Han
DOI
:10.4103/0973-1296.210166
PMID
:28808376
Background:
Helicobacter pylori (H. pylori)
is linked to the development of the majority of peptic ulcers and some types of gastric cancers, and its antibiotic resistance is currently found worldwide.
Objective:
This study is aimed at evaluating the anti-
H. pylori
activity of Korean acacia honey and isolating the related active components using organic solvents.
Material and Methods:
The crude acacia honey was extracted with
n
-hexane, dichloromethane, ethyl acetate (EtOAc), and
n
-butanol. The EtOAc extract was subjected to octadecyl-silica chromatography. The extracts and fractions were then examined for anti-
H. pylori
activity using the agar well diffusion method. The antimicrobial activity of abscisic acid against
H. pylori
was investigated by determining the minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), and by performing a time-kill assay.
Results:
Abscisic acid related to the botanical origins of acacia honey from Korea has been analyzed using ultra-performance liquid chromatography. The MICs and MBCs of abscisic acid were 2.7 ± 1.3 and 6.9 ± 1.9 μg/mL, respectively. The bactericidal activity of abscisic acid (at 10.8 μg/mL corresponding to 4 × MIC) killed the organism within 36–72 h. These results suggest that abscisic acid isolated from Korean acacia honey has antibacterial activity against
H. pylori
.
Conclusion:
Abscisic acid isolated from Korean acacia honey can be therapeutic and may be further exploited as a potential lead candidate for the development of treatments for
H. pylori
-induced infections.
Abbreviations used:
MeOH: Methanol; EtOAc: Ethyl acetate; TSB: Trypticase soy broth; MIC: Minimum inhibitory concentration; MBC: Minimum bactericidal concentration; CFU: Colony-forming units; UPLC: Ultra-performance liquid chromatography; DAD: Diode array detector; UV: Ultraviolet; ODS: Octadecyl-silica; MS: Mass spectrometry; SE: Standard error.
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Anti-inflammatory potential of
Petiveria alliacea
on activated RAW264.7 murine macrophages
p. 174
Rosa Martha Perez Gutierrez, Carlos Hoyo-Vadillo
DOI
:10.4103/pm.pm_479_16
PMID
:28808377
Background:
Defense and protection to multiple harmful stimuli are the inflammation, when is self-amplified and uncontrolled is the basis of the pathogenesis of a wide variety of inflammatory illness. The aim of this study was to evaluate if
Petiveria alliacea
could attenuate inflammation in a murine model of RAW264 macrophages the involved model and its involved mechanism.
Materials and Methods:
The ethanol extract from
P. alliacea
was precipitated with water and supernatant was used for this study (PW). The anti-inflammatory effects of PW were investigated through evaluating of the production of several cytokines, chemokines, and expression of nuclear factor-kappa B (NF-κB) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Also was determined the ability to decrease the oxidative stress in RAW264.7 cells with carboxy-2',7'-dichloro-dihydro-fluorescein diacetate.
Results:
PW significantly suppress the secretion of prostaglandin E
2
, leukotriene C
4
, interleukin (IL)-1 β, IL-6, IL-10, interferon gamma nitric oxide (NO), inducible NO synthase, IL-1 β, IL-4, in RAW264.7 cells in a dose-dependent manner. In addition, PW also markedly inhibited the transcriptional activity of NF-κB. PW produced significant anti-inflammatory activity through inhibiting the production of inflammatory mediators through the NF-κB inactivation in the LPS-stimulated RAW24.7 cells.
Conclusions:
PW exerts significant antioxidant and anti-inflammatory activities, and this effect can be attributed in part, to the presence of dibenzyl disulfide, dibenzyl trisulfide pinitol, coumarin, myricetin, glutamyl-S-benzyl cysteine, and petiveriins A and B.
Abbreviations used:
COX-2: Ciclooxigenasa 2; DCFHDA: Carboxy-2',7'-dichloro-dihydro-fluorescein diacetate; DMEM: Dulbecco's modified eagle's medium; FBS: Fetal bovine serum; HSP70: Heat shock protein; IFN-γ: Interferon gamma; IL-1 β: Interleukin 1 β, IL-6: Interleukin 6; IL-10: Interleukin 10; IL-4: Interleukin 4; iNOS: Nitric oxide synthase; KCl: Potassium chloride; LPS: Lipopolysaccharides; LTC4: leukotriene C 4; MgCl
2
: Magnesium chloride; MTT: 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide; NF-κB: Nuclear factor kappa-light-chain-enhancer of activated B-cells or transcriptional activity of nuclear factor-kB; NO: Nitric oxide; PBS: Phosphate-buffered saline; PGE2: Prostaglandin E2, PMSF: Phenylmethylsulfonyl fluoride; PTC: Chloroform extract from
Petiveria alliacea
; PE: Ethanol extract from
Petiveria alliacea
; PTH: Hexane extract from
Petiveria alliacea
; PW: Supernatant of PTE precipitated with water; RAW264.7: Cell line murine macrophages; ROS: Reactive oxygen species; TNF-α: Tumor necrosis factor.
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Rutamarin, an active constituent from
Ruta angustifolia
Pers., induced apoptotic cell death in the HT29 colon adenocarcinoma cell line
p. 179
Shafinah Ahmad Suhaimi, Sok Lai Hong, Sri Nurestri Abdul Malek
DOI
:10.4103/pm.pm_432_16
PMID
:28808378
Background:
Ruta angustifolia
Pers. is a perennial herb that is cultivated worldwide, including Southeast Asia, for the treatment of various diseases as traditional medicine.
Objective:
The purpose of the study was to identify an active principle of
R. angustifolia
and to investigate its effect on the HT29 cell death.
Materials and Methods:
The methanol and fractionated extracts (hexane, chloroform, ethyl acetate, and water) of
R. angustifolia
Pers. were initially investigated for their cytotoxic activity against two human carcinoma cell lines (MCF7 and HT29) and a normal human colon fibroblast cell line (CCD-18Co) using sulforhodamine B cytotoxicity assay. Eight compounds including rutamarin were isolated from the active chloroform extract and evaluated for their cytotoxic activity against HT29 human colon carcinoma cell line and CCD-18Co noncancer cells. Further studies on the induction of apoptosis such as morphological examinations, biochemical analyses, cell cycle analysis, and caspase activation assay were conducted in rutamarin-treated HT29 cells.
Results:
Rutamarin exhibited remarkable cytotoxic activity against HT29 cells (IC
50
value of 5.6 μM) but was not toxic to CCD-18Co cells. The morphological and biochemical hallmarks of apoptosis including activation of caspases 3, 8, and 9 were observed in rutamarin-treated HT29 cells. These may be associated with cell cycle arrest at the G0/G1 and G2/M checkpoints, which was also observed in HT29 cells.
Conclusions:
The present study describes rutamarin-induced apoptosis in the HT29 cell line for the first time and suggests that rutamarin has the potential to be developed as an anticancer agent.
Abbreviations used:
ACN: Acetonitrile, ANOVA: One-way analysis of variance, BrdU: Bromodeoxyuridine, 13C-NMR: Carbon-13 Nuclear magnetic resonance, CAD: Caspase-activated endonuclease, CCD-18Co: Human colon normal, DLD1: Human Duke's type C colorectal adenocarcinoma, DMRT: Duncan's multiple range test, DMSO: Dimethyl sulfoxide, DNA: Deoxyribonucleic acid, DR4/5: Death receptor 4/5 protein, EMEM: Eagle's minimum essential media, FBS: Fetal bovine serum, FITC Annexin V: Annexin V conjugated with fluorescein isothiocyanate, FITC-DEVD-FMK: Fluorescein isothiocyanate conjugate of caspase inhibitor Asp-Glu-Val-Asp-fluoromethyl ketone, FITC-IETD-FMK: Fluorescein isothiocyanate conjugate of caspase inhibitor Ile-Glu-Thr-Asp-fluoromethyl ketone, FITC-LEHD-FMK: Fluorescein isothiocyanate conjugate of caspase inhibitor Leu-Glu-His-Asp-fluoromethyl ketone, G0: Quiescent phase of cell cycle, G1: Gap 1 phase of cell cycle, G2: Gap 2 phase of cell cycle, GC-MS: Gas chromatography-mass spectrometry, HeLa: Human cervical adenocarcinoma, HPLC: High performance liquid chromatography, HT29: Human colon adenocarcinoma, Huh7.5: Human hepatocellular carcinoma, IC50: Half maximal inhibitory concentration, KSHV: Kaposi's sarcoma-associated herpesvirus, M phase: Mitotic phase of cell cycle, MCF7: Human breast adenocarcinoma, NMR: Nuclear magnetic resonance, PBS: Phosphate-buffered saline, PI: Propidium iodide, RNase: Ribonuclease, rt: Retention time, S phase: Synthesis phase of cell cycle, SD: Standard deviation, SRB: Sulforhodamine B, TCA: Trichloroacetic acid, TLC: Thin layer chromatography, TNF-R1: Tumor necrosis factor receptor 1 protein, TUNEL: Terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling, UV: Ultraviolet.
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Screening of fruits of seven plants indicated for medicinal use in Iraq
p. 189
Omar Aldulaimi
DOI
:10.4103/pm.pm_503_16
PMID
:28808379
Introduction:
Coumarins exert many biological effects in humans, animals, and plants, which make the evaluation of their biological activities and study of their role in ethnomedicine highly valued.
Objectives:
Here, we selected seven plants which have ethnopharmacological use as antimicrobial in Iraq and the aims were to quantify the two structural isomers bergapten and methoxsalen in their seeds, to evaluate the antibacterial activities against several clinical isolates, and to isolate bergapten and methoxsalen from
Ammi majus
.
Materials and Methods:
Seven plants were extracted by petroleum ether (PE) and ethanol (EtOH). Bergapten and methoxsalen were separated and purified by preparative thin-layer chromatography. Quantification of the furanocoumarins has been conducted by high-performance liquid chromatography, and all the plant extracts and pure compounds were checked for antibacterial activities utilizing alamar blue microplate assay.
Results:
Cuminum cyminum
was deprived of bergapten and methoxsalen and methoxsalen was not detected from
Apium graveolens
. Bergapten was abundant in PE more than in EtOH; on the other hand, EtOH was rich in methoxsalen. The separation of the two structural isomers was performed using normal phase chromatography and ultraviolet light as an indicator. All extracts showed weak to moderate antibacterial activities against Gram-positive isolates which were more sensitive than the negative ones.
C. cyminum
extract was least active, uncover the antibacterial role of bergapten and methoxsalen.
Conclusion:
These findings support the medicinal use of seeds of seven plants from
Apiaceae
family and quantify the two pharmacologically important furanocoumarins (bergapten and methoxsalen).
Abbreviations used:
EtOH: Ethanol; MIC: Minimum inhibitory concentration; PE: Petroleum ether; Rf: Retardation factor; Rt: Retention time.
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Identification and analysis of jasmonate pathway genes in
Coffea canephora
(robusta coffee) by in silico approach
p. 196
Kosaraju Bharathi, HL Sreenath
DOI
:10.4103/pm.pm_518_16
PMID
:28808380
Background:
Coffea canephora
is the commonly cultivated coffee species in the world along with
Coffea arabica
. Different pests and pathogens affect the production and quality of the coffee. Jasmonic acid (JA) is a plant hormone which plays an important role in plants growth, development, and defense mechanisms, particularly against insect pests. The key enzymes involved in the production of JA are lipoxygenase, allene oxide synthase, allene oxide cyclase, and 12-oxo-phytodienoic reductase. There is no report on the genes involved in JA pathway in coffee plants.
Objective:
We made an attempt to identify and analyze the genes coding for these enzymes in
C. canephora.
Materials and Methods:
First, protein sequences of jasmonate pathway genes from model plant
Arabidopsis thaliana
were identified in the National Center for Biotechnology Information (NCBI) database. These protein sequences were used to search the web-based database Coffee Genome Hub to identify homologous protein sequences in
C. canephora
genome using Basic Local Alignment Search Tool (BLAST).
Results:
Homologous protein sequences for key genes were identified in the
C. canephora
genome database. Protein sequences of the top matches were in turn used to search in NCBI database using BLAST tool to confirm the identity of the selected proteins and to identify closely related genes in species. The protein sequences from
C. canephora
database and the top matches in NCBI were aligned, and phylogenetic trees were constructed using MEGA6 software and identified the genetic distance of the respective genes. The study identified the four key genes of JA pathway in
C. canephora
, confirming the conserved nature of the pathway in coffee. The study expected to be useful to further explore the defense mechanisms of coffee plants.
Conclusion:
JA is a plant hormone that plays an important role in plant defense against insect pests. Genes coding for the 4 key enzymes involved in the production of JA viz., LOX, AOS, AOC, and OPR are identified in
C. canephora
(robusta coffee) by bioinformatic approaches confirming the conserved nature of the pathway in coffee. The findings are useful to understand the defense mechanisms of C. canephora and coffee breeding in the long run.
Abbreviations used:
C. canephora
:
Coffea canephora
;
C. arabica
:
Coffea arabica
; JA: Jasmonic acid; CGH: Coffee Genome Hub; NCBI: National Centre for Biotechnology Information; BLAST: Basic Local Alignment Search Tool;
A. thaliana
:
Arabidopsis thaliana
; LOX: Lipoxygenase, AOS: Allene oxide synthase; AOC: Allene oxide cyclase; OPR: 12 oxo phytodienoic reductase.
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Characterization, antibacterial and antioxidant properties of silver nanoparticles synthesized from aqueous extracts of
Allium sativum, Zingiber officinale
, and
Capsicum frutescens
p. 201
Gloria Aderonke Otunola, Anthony Jide Afolayan, Emmanuel Olusegun Ajayi, Samuel Wale Odeyemi
DOI
:10.4103/pm.pm_430_16
PMID
:28808381
Background:
Herbal drug delivery is limited by poor solubility and bioavailability which can be overcome with suitable nanomaterials that will enhance their pharmacokinetics and performance.
Objective:
This study aimed to analyze the synthesis, characterization, and biological activities of silver nanoparticles (AgNPs) from three spices.
Materials and Methods:
AgNPs were prepared using 0.1 M silver nitrate and aqueous extracts of
Allium sativum
L. (garlic),
Zingiber officinale
Rosc. (ginger), and
Capsicum frutescens
L. (cayenne pepper). The AgNPs were characterized using ultraviolet-visible (UV-Vis) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray, X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.
Results:
The AgNPs were formed within an hour of the reaction and showed maximum UV-Vis absorption in the 375–480 nm range. SEM and TEM revealed well-dispersed spherical particles with little agglomeration, average sizes of 3–6 nm, 3–22 nm, and 3–18 nm for garlic, ginger, and cayenne pepper, respectively. FTIR showed that amine, protein, phenolic, aromatic, and alkynes groups contributed to AgNP synthesis and XRD confirmed their crystalline and face-centered cubic nature. Antibacterial action of the AgNPs was in the following order: ginger (minimum inhibitory concentration [MIC] <25 μg/mL) > garlic> cayenne pepper (MIC 125 μg/mL). Antioxidant action showed cayenne pepper > ginger > garlic (inhibitory concentration 50% [IC50]: 40, 240, and 250 μg/mL, respectively) against 2,2-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) and garlic > cayenne pepper > ginger (IC50: <31.25, 40, and 120 μg/mL, respectively) against 1,1-diphenyl-2-picrylhydrazyl.
Conclusion:
Optimization of this green synthesis would support the production of AgNPs with great therapeutic potentials.
Abbreviations used:
AgNPs: Silver nanoparticles; UV-Vis: ultraviolet-visible; SEM: Scanning electron microscopy; TEM: Transmission electron microscopy; EDX: Energy dispersive X-ray; XRD: X-ray diffraction; FTIR: Fourier transform infrared; GaNPs: Garlic nanoparticles; GiNPs: Ginger nanoparticles; C.PeNPs: Cayenne pepper nanoparticles; FCC: Face centred cubic; SPR: Surface Plasmon resonance; ABTS-2: 2-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid); DPPH-1: 1-diphenyl-2-picrylhydrazyl.
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Allium sativum
constituents exhibit anti-tubercular activity
In vitro
and in RAW 264.7 mouse macrophage cells infected with
Mycobacterium tuberculosis
H37Rv
p. 209
Swapna S Nair, Sujay S Gaikwad, Savita P Kulkarni, Alka Pravin Mukne
DOI
:10.4103/pm.pm_435_16
PMID
:28808382
Background:
Long duration of treatment, side-effects of currently used anti-tubercular drugs and emergence of drug-resistant forms of
Mycobacterium tuberculosis
(MTB) warrants the need to develop new drugs to tackle the scourge of tuberculosis (TB). Garlic is an edible plant reported to have anti-tubercular activity. However, previous researches on anti-tubercular effect of garlic were focused mostly on preliminary
in vitro
screening.
Objective:
To identify constituents responsible for anti-tubercular activity of thiosulfinate-derivative rich extract of garlic (GE) and to evaluate activity of the most active constituent in RAW 264.7 mouse macrophage cells infected with
M. tuberculosis
H37Rv (MTBH).
Materials and Methods:
In the present study, we have isolated eight compounds from GE by flash chromatography. The isolated compounds were characterized by
1
H nuclear magnetic resonance spectroscopy, liquid chromatography-mass spectrometry and Fourier transform infrared spectroscopy. Individual isolates and GE were screened for activity against MTBH by Resazurin Microtitre Plate Assay (REMA).
Results:
Anti-tubercular activity of GE was superior to that of isolates when evaluated by REMA, possibly due to synergism amongst the constituents of GE. Cytotoxicity of GE was evaluated in RAW 264.7 mouse macrophage cells and it was observed that GE had a favorable selectivity index (>10). Therefore, anti-tubercular activity of GE was further evaluated by intracellular macrophage infection model. GE demonstrated concentration-dependent activity in macrophages infected with MTBH.
Conclusion:
This is the first report on intracellular anti-tubercular activity of any extract of garlic or its components. Appreciable intracellular anti-tubercular activity of GE in macrophages combined with low cytotoxicity makes it a suitable candidate for further development as an anti-tubercular agent.
Abbreviations used:
TB: Tuberculosis, MTB:
Mycobacterium tuberculosis
, MTBH:
Mycobacterium tuberculosis
H37Rv, GE: Thiosulfinate-derivative rich extract of garlic, REMA: Resazurin Microtitre Plate Assay, VD: Vinyldithiin, CFU: Colony forming unit,
1
H NMR:
1
H nuclear magnetic resonance spectroscopy, FT-IR: Fourier transform-infrared spectroscopy, LC-MS: Liquid chromatography-mass spectrometry, IC
50
: Concentration required to inhibit the cells by 50%, ANOVA: Analysis of variance.
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Chemopreventive potential of major flavonoid compound of methanolic bark extract of
Saraca asoca
(Roxb.) in benzene -induced toxicity of acute myeloid leukemia mice
p. 216
Manas Kumar Mukhopadhyay, Mithun Shaw, Debjani Nath
DOI
:10.4103/pm.pm_326_15
PMID
:28808383
Background:
Saraca asoca
(SA) (Roxb.) is one of the folk medicinal plants found in India, Bangladesh, and Sri Lanka. Its major biological activity appears due to the presence of flavonoid group of compounds in its bark extract.
Objective:
In this study, our research aims to analyze the chemopreventive effect of flavonoids, especially a natural phenol catechin present in the bark methanolic extract of SA on acute myeloid leukemia (AML) mice.
Materials and Methods:
The total bark extract was partitioned and analyzed on thin-layer chromatography (TLC) plate. The yellow-brown material of spot 4 was analyzed and identified as catechin. The yellowish brown material (YBM) was tested for their chemopreventive potential. An
in vivo
AML mice model was used to test the efficacy. Hematological parameters (Hb %, red blood cell, and white blood cell count), expression of cell cycle regulatory proteins, and DNA fragmentation analysis were performed.
Results:
After treatment of benzene-exposed mice with the major flavonoid compound, namely catechin, the above parameters increase significantly (
P
< 0.05). There was an upregulation of p53 and p21, caspase 11 myeloperoxidase, bcl2, and CYP2EI in catechin-treated group. DNA was less fragmented in flavonoid-treated group compared to that of control (
P
≤ 0.05). The present study indicates that the secondary metabolites of SA methanolic bark extract, comprising flavonoid catechin as major constituents, have modulatory effect in cell cycle deregulation and hematological abnormalities induced by benzene in mice.
Conclusions:
Our data suggest that catechin from methanolic bark extract of SA effectively attenuates benzene-induced secondary AML in bone marrow, which is likely associated with the anticell cycle deregulation properties of this flavan-3-ol. This study was supported by the observation that catechin (YBM), like doxorubicin, can act as the neutralizer and protector of mortality in cancer cases.
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Molecular authentication of the traditional medicinal plant “Lakshman Booti” (
Smithia conferta
Sm.) and its adulterants through DNA barcoding
p. 224
Suraj D Umdale, Parthraj R Kshirsagar, Manoj M Lekhak, Nikhil B Gaikwad
DOI
:10.4103/pm.pm_499_16
PMID
:28808384
Background:
Smithia conferta
Sm. is an annual herb widely used in Indian traditional medical practice and commonly known as “Lakshman booti” in Sanskrit. Morphological resemblance among the species of genus
Smithia Aiton
. leads to inaccurate identification and adulteration. This causes inconsistent therapeutic effects and also affects the quality of herbal medicine.
Aim:
This study aimed to generate potential barcode for authentication of
S. conferta
and its adulterants through DNA barcoding technique.
Materials and Methods:
Genomic DNA extracted from
S. conferta
and its adulterants was used as templates for polymerase chain reaction amplification of the barcoding regions. The amplicons were directed for sequencing, and species identification was conducted using BLASTn and unweighted pair-group method with arithmetic mean trees. In addition, the secondary structures of internal transcribed spacer (ITS) 2 region were predicted.
Results:
The nucleotide sequence of ITS provides species-specific single nucleotide polymorphisms and sequence divergence (22%) than
psb
A-
trn
H (10.9%) and
rbc
L (3.1%) sequences. The ITS barcode indicates that
S. conferta
and
Smithia sensitiva
are closely related compared to other species.
Conclusion:
ITS is the most applicable barcode for molecular authentication of
S. conferta,
and further chloroplast barcodes should be tested for phylogenetic analysis of genus
Smithia
.
Abbreviations used:
BLASTn: Basic Local Alignment Search Tool for Nucleotide; MEGA: Molecular Evolutionary Genetic Analysis; EMBL: European Molecular Biology Laboratory;
psb
A-
trn
H: Photosystem II protein D1- stuctural RNA: His tRNA gene; rbcL: Ribulose 1,5 bi-phosphate carboxylase/ oxygenase large subunit gene.
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Anti-inflammatory effect of
Etlingera pavieana
(Pierre ex Gagnep.) R.M.Sm. rhizomal extract and its phenolic compounds in lipopolysaccharide-stimulated macrophages
p. 230
Ekaruth Srisook, Mullika Palachot, Sakulrat Mankhong, Klaokwan Srisook
DOI
:10.4103/pm.pm_558_16
PMID
:28808385
Background:
In our continuing search for anti-inflammatory agents from Thai herbs,
Etlingera pavieana
(Pierre ex Gagnep.) R.M.Sm. showed potent inhibition on nitric oxide (NO) production in lipopolysaccharide (LPS)-induced macrophages. However, the mechanism behind its inhibitory effect has not been yet explored, and little is known regarding its bioactive compounds responsible for the anti-inflammatory effect.
Objective:
In the present study, anti-inflammatory effect of hexane, ethyl acetate, and water fractions of rhizomal ethanol extracts of
E. pavieana
was evaluated for their inhibition on NO production and mechanism in LPS-stimulated macrophages. Active compounds responsible for such anti-inflammatory activity were identified.
Materials and Methods:
Inhibitory activities on NO production were performed in LPS-stimulated RAW264.7 macrophage. Cytotoxicity of plant extracts was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, mRNA and protein expressions by reverse transcription-polymerase chain reaction and Western blotting analysis, respectively. Anti-inflammatory compounds were isolated by activity-guided isolation technique using column chromatography.
Results:
Ethyl acetate fraction of
E. pavieana
(EPE) showed the most potent inhibitory effect on NO production in macrophages. EPE significantly decreased NO production and inhibited inducible nitric oxide synthase (iNOS) protein and mRNA expression in a dose-dependent manner. Furthermore, the level of nuclear factor-kappa B p65 subunit was markedly reduced in activated cells treated with EPE. Four phenolic compounds, 4-methoxycinnamyl alcohol (1), trans-4-methoxycinnamaldehyde (2), 4-methoxycinnamyl
p
-coumarate (3), and
p
-coumaric acid (4), were obtained from bioactivity-guided isolation technique.
Conclusions:
The anti-inflammatory property contained in
E. pavieana
rhizome extract and conferred through inhibition of iNOS expression, and NO formation provides scientific evidence and support for the development of new anti-inflammatory agents based on extracts from this plant.
Abbreviations used:
EPE: Ethyl acetate fraction of
Etlingera pavieana
; EPH: Hexane fraction of
Etlingera pavieana
; EPW: Water fraction of
Etlingera pavieana
; NO: Nitric oxide (NO); LPS: Lipopolysaccharide; iNOS: Inducible nitric oxide synthase (iNOS); MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NF-κB: Nuclear factor-kappa B; DMSO: Dimethyl sulfoxide; EtOAc: Ethylacetate; MeOH: Methanol; AG: Aminoguanidine; DCM: Dichloromethane; MCA: 4-methoxycinnamyl alcohol; MCD: trans-4-methoxycinnamaldehyde; MCC: 4-methoxycinnamyl p-coumarate; CM: p-coumaric acid.
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In vitro
inhibitory effects of
Andrographis paniculata, Gynura procumbens, Ficus deltoidea
, and
Curcuma xanthorrhiza
extracts and constituents on human liver glucuronidation activity
p. 236
Zulhilmi Husni, Sabariah Ismail, Mohd Halimhilmi Zulkiffli, Atiqah Afandi, Munirah Haron
DOI
:10.4103/pm.pm_299_16
PMID
:28808386
Background:
Andrographis paniculata
,
Gynura procumbens
,
Ficus deltoidea
and
Curcuma xanthorrhiza
are commonly consumed as herbal medicines. However their effects on human liver glucuronidation activity are not yet evaluated.
Objective:
In this study, we evaluate the inhibitory Effects of Andrographis paniculata, Gynura procumbens, Ficus deltoidea and Curcuma xanthorrhiza extracts and their constituents on human liver glucuronidation activity.
Materials and Methods:
Herbal extracts (aqueous, methanolic and ethanolic extracts) and their constituents were incubated with human liver microsomes with the addition of UDPGA to initiate the reaction. Working concentrations of herbal extracts and their constituents ranged from 10 μg/mL to 1000 μg/mL and 10 μM to 300 μM respectively. IC50 was determined by monitoring the decrement of glucuronidation activity with the increment of herbal extracts or phytochemical constituent's concentrations.
Results:
All herbal extracts inhibited human liver glucuronidation activity in range of 34.69 μg/mL to 398.10 μg/mL whereas for the constituents, only xanthorrhizol and curcumin (constituents of
Curcuma xanthorrhiza
) inhibited human liver glucuronidation activity with IC50 of 538.50 and 32.26 μM respectively.
Conclusion:
In the present study, we have proved the capabilities of
Andrographis paniculata
,
Gynura procumbens
, Ficus deltoidea and
Curcuma xanthorrhiza
to interfere with i
n vitro
glucuronidation process in human liver microsomes.
Abbreviations used:
UGT: Uridine 5'-diphospho-glucuronosyltransferase; 4-MU: 4-methylumbelliferone; IC50: Half Maximal Inhibitory Concentration; Km: Michaelis constant; Vmax: Maximum velocity.
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New apigenin glycoside, polyphenolic constituents,anti-inflammatory and hepatoprotective activities of
Gaillardia grandiflora
and
Gaillardia pulchella
aerial parts
p. 244
Fatma A Moharram, Rabab Abd El Moneim El Dib, Mohamed S Marzouk, Siham M El-Shenawy, Haitham A Ibrahim
DOI
:10.4103/pm.pm_344_16
PMID
:28808387
Background:
Gaillardia grandiflora
Hort. ex Van Houte and
Gaillardia pulchella
Foug are flowering plants widely cultivated in Egypt for their ornamental value. Previous reports demonstrated that sesquiterpene derivatives represent the major compounds in both species. Moreover, only few flavones were identified from genus
Gaillardia
and few studies on the cytotoxicity of
G. pulchella
were found.
Aim of the Study:
Investigation of the phenolic constituents of the aerial parts of both species and evaluation of their anti-inflammatory and hepatoprotective activities.
Materials and Methods:
The 80% aqueous methanol extracts (AME) were prepared for both plants and evaluated for their biological activities. Phytochemical investigation of both extracts resulted in isolation of twelve compounds, which have been identified on the basis of ultraviolet, 1D and 2D nuclear magnetic resonance spectroscopy and negative ESI-MS.
Results:
The new 8-hydroxyapigenin 6-O-β-D-apiofuranosyl-(1'''→6'')-C-β-D-
4
C
1
-glucopyranoside was isolated from
G. grandiflora
for the first time in nature, along with schaftoside, luteolin 6-C-β-D-
4
C
1
-glucopyranoside 8-methyl ether, apigenin 6-C-β-D-
4
C
1
-glucopyranoside 8-methyl ether, isoorientin, isovitexin, 6-methoxyluteolin and hispidulin, as well as vicenin-2, vitexin, luteolin and apigenin, which were isolated from
G. pulchella
together with 6-methoxyluteolin. Furthermore, the AME of both species were found to be nontoxic to mice and exhibited significant anti-inflammatory and hepatoprotective activities in dose dependent manner.
Conclusion:
Current results shed light on the phenolic constituents of
G. grandiflora
and
G. pulchella
aerial parts and the safety of the AME of both species, in addition to their significant anti-inflammatory and hepatoprotective activities. Both plant species may be promising candidates for natural anti-inflammatory and hepatoprotective drugs.
Abbreviations used:
ALP: Alkaline phosphatase; ALT: Alanine aminotransferase; AME: The 80% aqueous methanol extract of
G. grandiflora
or
G. pulchella aerial
parts; AST: Aspartate aminotransferase; br d: Broad doublet; Comp-PC: Comparative paper chromatography; d: Doublet; 2D-PC: Two-dimensional paper chromatography; DMSO-d6: Deuterated dimethyl sulfoxide;
G.: Gaillardia
; GPx: Glutathione peroxidase; GRd: Glutathione reductase; GSH: glutathione; GST: Glutathione-S-transferase;
J
: Nuclear spin-spin coupling constant; m: Multiplet; [M-H].: Molecular ion peak; MDA: Malondialdehyde;
m/z
: Mass/charge ratio; NO: Nitric oxide; p: Probability; PC: Paper chromatography; Rf: Retention flow; rpm: Rotation per minute; s: Singlet; SDE: The ethanol extract of
Scoparia dulcis
; SE: Standard error; SOD: Superoxide dismutase; TMS: Tetramethylsilane; γmax: Maximum fluorescence emission wavelength.
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Caesalpinia Crista
Linn. Induces protection against DNA and membrane damage
p. 250
R Sunil Kumar, Ramesh Balenahalli Narasingappa, Chandrashekhar G Joshi, Talakatta K Girish, Ananda Danagoudar
DOI
:10.4103/pm.pm_557_16
PMID
:28808388
Background:
Caesalpinia crista
is a medicinal herb used to cure various ailments in subtropical and tropical regions of Southeast Asia.
Objective:
The objective of this evaluation of
C. crista
against free radical induced DNA and erythrocyte damage.
Materials and Methods:
The profiles of polyphenol and flavonoid were quantified through reversed-phase high-performance liquid chromatography. Free radical induced DNA and membrane damage were performed using H
2
O
2
as oxidative agent.
Results:
The total polyphenol content of
C. crista
leaf ethyl acetate extract (CcEA) was 94.5 ± 3.8 mg/gGAE, CcME (
C. crista
leaf methanol extract) was 52.7 ± 2.8 mg/gGAE, and CcWE (
C. crista
leaf Water extract) was 31.84 ± 1.8 mg/gGAE. Total flavonoid content of CcEA was 60.46 ± 2.3 mg/gQE, CcME was 46.26 ± 1.8 mg/gQE, and CcWE was 20.47 ± 1.1 mg/gQE. The extracts also exhibited good antioxidant activity as confirmed by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid), hydroxyl scavenging, reducing power, and total antioxidant assays. Among the three extracts, CcEA and CcME showed better protection against red blood cell (RBC) hemolysis and DNA damage as confirmed by electrophoretic study. Further, Scanning electron micrograph data showed that CcEA revealed the free radical induced structural alterations in RBC.
Conclusion:
These findings suggest that
C. crista
contains bioactive molecules and can inhibit oxidative stress and can be source of further study to use this in herbal medicine.
Abbreviations used:
ABTS: 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid); CcEA: C. crista leaf ethyl acetate extract; CcME: C. crista leaf methanol extract; CcWE: C. crista leaf Water extract; DPPH: 2,2-diphenyl-1-picrylhydrazyl; GAE: Gallic acid Equivalent; H
2
O
2
: Hydrogen Peroxide; QE: Quercetin Equivalent; RNS: Reactive Nitrogen Spevcies; ROS: Reactive Oxygen Species; SEM: Scanning Electron Microscope.
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Simultaneous determination of four compounds, campesterol, emodin8-O-β-D-Glucopyranoside, quercetin, and isoquercitrin in
Reynoutria sachalinensis
by high-performance liquid chromatography-diode array detector
p. 258
Min Rye Eom, Jin Bae Weon, Youn Sik Jung, Ga Hee Ryu, Woo Seung Yang, Choong Je Ma
DOI
:10.4103/pm.pm_289_16
PMID
:28808389
Background:
Reynoutria sachalinensis
is a well-known and used herbal medicine to treatment of arthralgia, jaundice, amenorrhea, coughs, carbuncles, and sores. Objective: We have developed high-performance liquid chromatography analysis method for simultaneous determination of isolated four compounds, campesterol, emodin8-O-β-D-glucopyranoside, quercetin, and isoquercitrin from
R. sachalinensis
is.
Materials and Methods:
The four compounds were separated on Shiseido C
18
column (S-5 μm, 4.6 mm I.D. ×250 mm) at a column temperature of 25°C. The mobile phase composed of water and methanol with gradient elution system, and flow rate is 1.0 ml/min. The detection wavelength was set at 205 nm.
Results:
Validation of this analytical method was evaluated by linearity, precision, and accuracy test. This established method had good linearity (
R
2
> 0.997). The relative standard deviation values of intra- and inter-day testing were indicated that <2%, and accuracy is 91.66%–103.31% at intraday and 91.69%–103.31% at intraday. The results of recovery test were 92.60%–108.99%.
Conclusion:
In these results, developed method was accurate and reliable to the quality evaluation of campesterol, emodin 8-O-β-D-glucopyranoside, quercetin, and isoquercitrin isolated from
R. sachalinensis.
Abbreviations used:
HPLC: High-performance liquid chromatography, DAD: Diode array detector, LOD: Limit of detection, LOQ: Limit of quantitation, ICH: International Conference on Harmonisation.
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Immunomodulatory, cytotoxicity, and antioxidant activities of roots of
Ziziphus mauritiana
p. 262
Samina Afzal, Murium Batool, Bashir Ahmad Ch, Ashfaq Ahmad, Muhammad Uzair, Khurram Afzal
DOI
:10.4103/pm.pm_398_16
PMID
:28808390
Aims:
The study is conducted to evaluate the immunomodulatory, cytotoxicity, and antioxidant potential of
Ziziphus mauritiana
(Rhamnaceae). Phytochemical analysis of
Z. mauritiana
revealed the presence of alkaloids, anthraquinone glycoside, cardiac glycoside, saponin, tannin, and flavonoids.
Methodology:
The cytotoxicity of the plant
Z. mauritiana
was evaluated by brine shrimp lethality test. Antioxidant parameters such as superoxide dismutase (SOD), total antioxidant capacity (T-AOC), and malondialdehyde (MDA) levels were calculated in the plasma of rats after chronic administration of 400 mg/kg of
Z. mauritiana
for 6 weeks.
Results:
The dichloromethane extract of the plant exhibited significant immunomodulatory activity, with inhibitory concentration 50% of 55.43 ± 7.9. The dichloromethane extracts of the plant showed 70% mortality at concentration 1000 μg/ml. SOD and T-AOC levels were increased while MDA level in the plasma was reduced in the plasma of rats treated with dichloromethane
Z. mauritiana.
Conclusion:
This can be deduced that the root of
Z. mauritiana
has immunomodulatory, cytotoxic, and antioxidant potential.
Abbreviations used:
SOD: Superoxide dismutase; T-AOC: Total antioxidant capacity; MDA: Malondialdehyde; ZMRD:
Z. mauritiana
root extract of dichloromethane fraction; LD50:
Z. mauritiana
root extract of methanol fraction ZMRM, lethal dose 50.
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Resolving identification issues of
Saraca asoca
from its adulterant and commercial samples using phytochemical markers
p. 266
Satisha Hegde, Harsha Vasudev Hegde, Sunil Satyappa Jalalpure, Malleswara Rao Peram, Sandeep Ramachandra Pai, Subarna Roy
DOI
:10.4103/pm.pm_417_16
PMID
:28808391
Saraca asoca
(Roxb.) De Wilde (Ashoka) is a highly valued endangered medicinal tree species from Western Ghats of India. Besides treating cardiac and circulatory problems,
S. asoca
provides immense relief in gynecological disorders. Higher price and demand, in contrast to the smaller population size of the plant, have motivated adulteration with other plants such as
Polyalthia longifolia
(Sonnerat) Thwaites. The fundamental concerns in quality control of
S. asoca
arise due to its part of medicinal value (Bark) and the chemical composition. Phytochemical fingerprinting with proper selection of analytical markers is a promising method in addressing quality control issues. In the present study, high-performance liquid chromatography of phenolic compounds (gallic acid, catechin, and epicatechin) coupled to multivariate analysis was used. Five samples each of
S. asoca
,
P. longifolia
from two localities alongside five commercial market samples showed evidence of adulteration. Subsequently, multivariate hierarchical cluster analysis and principal component analysis was established to discriminate the adulterants of
S. asoca
. The proposed method ascertains identification of
S. asoca
from its putative adulterant
P. longifolia
and commercial market samples. The data generated may also serve as baseline data to form a quality standard for pharmacopoeias.
Abbreviations used:
HPLC: High Performance Liquid Chromatography; RP-HPLC: Reverse Phase High Performance Liquid Chromatography; CAT: Catechin; EPI: Epicatechin; GA: Gallic acid; PCA: Principal Component Analysis.
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Antiurolithiatic effect of
Sirupeelai Samoola Kudineer
: A polyherbal siddha decoction on ethylene glycol-induced renal calculus in experimental rats
p. 273
A Hannah Rachel Vasanthi, V Muthulakshmi, V Gayathri, R Manikandan, S Ananthi, Sarah Kuruvilla
DOI
:10.4103/pm.pm_454_16
PMID
:28808392
Background:
Sirupeelai Samoola Kudineer
(SK), a polyherbal decoction containing four medicinal plants has been used in Siddha system of medicine, practiced in Southern parts of India for the management of urolithiasis.
Objective:
The present study is carried out to scientifically validate the traditional claim and to study the mechanism of action of the drug.
Materials and Methods:
In the present study, anti-urolithiatic effect of SK was evaluated in Sprague-Dawley rats using ethylene glycol through drinking water and intraperitoneal injection of sodium oxalate. Renal damage was confirmed by the increased production of thiobarbituric acid reactive substance (TBARS).
Results:
Co-treatment with SK to urolithiatic rats for 21 days significantly prevented the elevation of renal and urinary stone biomarkers in plasma and renal tissue thereby preventing renal damage and the formation of renal calculi. Administration of SK at all doses and cystone restored the antioxidant (glutathione) levels by preventing the elevation of TBARS in the kidney tissue, which was further confirmed by histological sections.
Conclusions:
SK treatment promotes diuresis which leads to flushing of the renal stones and maintains the alkaline environment in the urinary system which probably mediates the antilithiatic activity. SK provides structural and functional protection to the kidneys by enhancing its physiological function against stone formation and validates its clinical use.
Abbreviations used:
SK: Sirupeelai Samoola Kudineer; TBARS: ThioBarbituric Acid Reactive Substances; SOD: SuperOxide Dismutase; GPx: Gluthathione peroxidase; GSH- Glutathione; LPO: Lipid peroxidation as measured as TBARS; AST: Aspartate AminoTransferase; ALT: Alanine Amino transferase; GGT: Gamma Glutamyl Transferase; LDH: Lactate Dehydrogenase.
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Evaluation of the antidiabetic properties of S-1708 mulberry variety
p. 280
Brijesh Ranjan, Randhir Kumar, Neeraj Verma, Swati Mittal, Pranab Lal Pakrasi, R Venkatesh Kumar
DOI
:10.4103/pm.pm_490_16
PMID
:28808393
Background:
Diabetes is a metabolic disease prevalent worldwide in all age group of people. The source of diabetes is due to an oxidation process that can produce free radicals. An increase in oxidative free radicals in the body is reported to be one of the several causes of diabetes. The best remedy to combat oxidative stress is the use of antioxidants, which inhibit and scavenge free radicals.
Aim:
This study has been undertaken to evaluate the antioxidant activity and antidiabetic effect of mulberry leaf extract in diabetic mice.
Materials and Methods:
Antioxidant activity of mulberry leaves was determined by 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) assay. Antidiabetic assay of mulberry leaf extract was analyzed by oral administration of leaf extract up to 3 weeks in diabetic mice induced by streptozotocin.
Results:
In vitro
antioxidant activity in both DPPH and FRAP assays showed significantly (
P
< 0.05) higher inhibition of free radicals than that with ascorbic acid. Diabetic mice fed with mulberry leaf extract showed increment (+25.88%) in body weight and a significant reduction in blood glucose concentration (−71.58%). Further, glucose-6-phosphate dehydrogenase enzyme activity was significantly (
P
< 0.05) increased, whereas activities of other enzymes particularly catalase, serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase were decreased in diabetic mice after oral administration of mulberry leaf extracts. Histology of liver revealed regeneration of hepatocytes, central vein, and nucleus.
Conclusion:
This study demonstrated that S-1708 mulberry variety has a potential therapeutic value in diabetes and related complications.
Abbreviations used:
S-1708, DPPH, FRAP.
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Antiproliferative activity of
Haematoxylum brasiletto
H. Karst
p. 289
J Bello-Martinez, M Jiménez-Estrada, JL Rosas-Acevedo, LP Avila-Caballero, M Vidal-Gutierrez, C Patiño-Morales, E Ortiz-Sánchez, RE Robles-Zepeda
DOI
:10.4103/pm.pm_466_16
PMID
:28808394
Background:
Haematoxylum brasiletto
is a tree that grows in Central America, commonly known as “Palo de Brasil,” which is used in the traditional medicine for the treatment of cancer and gastric ulcers.
Objective:
The aim of this study was to isolate the compounds responsible for antiproliferative activity of
H. brasiletto.
Materials and Methods:
A bioassay-guided fractionation of ethanol extract of
H. brasiletto
was performed using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide cell proliferation assay to measure the antiproliferative activity on six human cancer cell lines (A549, LS180, HeLa, SiHa, MDA-MB-231, and NCI-H1299) and one human noncancer cell line (ARPE-19). The ethanol extract was partitioned with hexane, dichloromethane, and ethyl acetate. The active dichloromethane fraction was fractioned by silica-column chromatography, and active subfractions were separated using preparative-thin layer chromatography. The chemical structure of an isolated compound was elucidated with different chemical and spectroscopic methods.
Results:
The flavonoid brazilin (1) was isolated from the heartwood of
H. brasiletto
. The measurement of antiproliferative activity showed that brazilin can inhibit the growth of SiHa, MDA-MB-231, A549, and NCI-H1299 cell lines by 50% at doses of 44.3, 48.7, 45.4, and 48.7 μM, respectively. Furthermore, the flavonoid showed a high antiproliferative activity on LS 180 and HeLa with IC50 values of 62.2 and 71.9 μM, respectively. Brazilin also exhibited a high antiproliferative activity on the human noncancer cell line ARPE-19 with an IC50 value of 37.9 μM.
Conclusions:
Brazilin: (6a
S
,11b
R
)-7,11b-Dihidro-6
H
-indeno[2,1-
c
] cromeno-3,6a, 9,10-tetrol was isolated; this compound demonstrated antiproliferative activity against several human cancer cell lines. This work demonstrated that brazilin, a flavonoid isolated and characterized of
H. brasiletto
, has antiproliferative activity against cancer cell lines.
Abbreviations used:
MTT: [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium]; FBS: Fetal bovine serum; TLC: Thin layer chromatography.
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Hepatoprotective effect of gallotannin-enriched extract isolated from gall on hydrogen peroxide-induced cytotoxicity in HepG2 cells
p. 294
Jun Go, Ji Eun Kim, Eun Kyoung Koh, Sung Hwa Song, Hyun Gu Kang, Young Hee Lee, Han Do Kim, Jin Tae Hong, Dae Youn Hwang
DOI
:10.4103/pm.pm_424_15
PMID
:28808395
Background:
Gall (Galla Rhois [GR]) is known to have antibacterial, anti-inflammatory, antimetastatic, and anti-invasion activities and exert hepatoprotective effects. However, the hepatoprotective effects of gallotannin-enriched GR (GEGR) and their mechanisms have not yet been investigated.
Objective:
The potential protective effect of GEGR against hepatotoxicity induced by hydrogen peroxide (H
2
O
2
) was investigated.
Materials and Methods:
Changes in cell viability, apoptosis protein expression, and reactive oxygen species (ROS) generation were determined in HepG2 cells that were pretreated with four different concentrations of GEGR (6.25–50 μg/ml) for 24 h before H
2
O
2
exposure.
Results:
GEGR consisted of gallotannin (69.2%), gallic acid (26.6%), and methyl gallate (4.2%) and showed remarkable 2,2-diphenyl-1-picrylhydrazyl scavenging activity (inhibitory concentration 50% = 0.212 μg/ml). The lethal dose 50% and effective dose 50% values for the response of HepG2 cells to GEGR were determined to be 178 and 6.85 μg/ml, respectively. Significant reductions in the immunofluorescence intensity indicating apoptosis were also detected in the nuclei of HepG2 cells stained with 4',6-diamidino-2-phenylindole and Annexin V after GEGR treatment. The Bax/Bcl-2 ratio and active caspase-3 level were higher in H
2
O
2
+ vehicle-treated cells. However, these levels gradually decreased to those of the No-treated group in the GEGR pretreated group even though little or no decrease was observed in response to low GEGR concentrations. Furthermore, the GEGR pretreated group showed a reduced level of 2-
′
,7-
′
-dichlorofluorescein diacetate stained cells, indicating ROS generation relative to the H
2
O
2
+ vehicle-treated group.
Conclusion:
The results of this study provide strong evidence that GEGR can prevent cell death induced by H
2
O
2
in HepG2 cells through the induction of antioxidant conditions.
Abbreviations used:
COX: Cyclooxygenase; DAPI: 4',6-diamidino-2-phenylindole; DMSO: Dimethyl sulfoxide; DPPH: 2,2-diphenyl-1-picrylhydrazyl; GEGR: Gallotannin-enriched Galla Rhois; GR: Galla Rhois; HPLC: High-performance liquid chromatography; H
2
O
2
: Hydrogen peroxide; MMP: Metallopeptidase; MTT: 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; ROS: Reactive oxygen species; UV-Vis: Ultraviolet-visible.
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Catechin isolated from
Garcinia celebica
leaves inhibit
Plasmodium falciparum
growth through the induction of oxidative stress
p. 301
Rizky Abdulah, Eka W Suradji, Anas Subarnas, Unang Supratman, Milyadi Sugijanto, Ajeng Diantini, Keri Lestari, Melisa I Barliana, Shinichiro Kawazu, Hiroshi Koyama
DOI
:10.4103/pm.pm_571_16
PMID
:28808396
Background:
Resistance of antimalarial drugs to
Plasmodium falciparum
has become a major concern in malaria eradication. Although it is also affected by several socioeconomic factors, a new antiplasmodial agent is needed for a global malaria control program.
Objective:
In this study, we attempted to uncover the antiplasmodial properties of
Garcinia celebica
, an Indonesian medicinal plant, along with the responsible compound and its possible mechanism.
Materials and Methods:
The
G. celebica
leaves were ethanol extracted and fractionated based on their polarity using
n
-hexane, ethyl acetate, and water. The antiplasmodial activity was tested
in vitro
against chloroquine-resistant
P. falciparum
at 100 μg/ml for 72 h. The active compound of the most active ethyl acetate fraction was subsequently isolated using column chromatography and identified by nuclear magnetic resonance.
Results:
The IC
50
of (+)-catechin, the characterized compound, against
P. falciparum
was 198 μM in 24 h and experiment. The isolated catechin inhibited
P. falciparum
growth in both trophozoite and schizont stages. An additional experiment also suggests that the antiplasmodial property of catechin occurs through the induction of the oxidative stress to
P. falciparum
.
Conclusion:
This result shows that the potential of catechin and its antimalarial properties should be explored further.
Abbreviations used:
RBC: Red Blood Cells; IC50: Inhibition Concentrattino 50; MeOH: Methanol; RPMI: Roswell Park Memorial Institute; EI: Electron Ionization.
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Genistein-attenuated gastric injury on indomethacin-induced gastropathy in rats
p. 306
Sarocha Vivatvakin, Duangporn Werawatganon, Kanjana Somanawat, Naruemon Klaikeaw, Prasong Siriviriyakul
DOI
:10.4103/pm.pm_502_16
PMID
:28808397
Objectives:
To investigates the mucoprotective effect of genistein on gastric injury in rats with indomethacin (IMN)-induced gastropathy.
Methods:
Male Sprague-Dawley rats were randomly divided into three groups. Group 1 (control; n = 6) was given distilled water (DW). Group 2 (IMN;
n
= 6) was given indomethacin (IMN) 150 mg/kg dissolved in 5% sodium bicarbonate (NaHCO
3
-
) 1 mL/rat via intragastric tube at time 0 and 4 h. Group 3 (genistein;
n
= 6) was given genistein 100 mg/kg dissolved in 0.1% dimethyl sulfoxide (DMSO) plus IMN 150 mg/kg at time described as group 2. Four hours after the second dose, the stomach was removed to examine iNOS western blot expression, malondialdehyde (MDA), and histopathologic examination. Serum was collected to determine TNF-alpha and prostaglandin E
2
(PGE
2
) levels using ELISA technique.
Results:
Tissue MDA and serum TNF-alpha were significantly increased in the IMN group, as compared to the control group (9.70 ± 0.40 vs. 1.56 ± 0.14 nmol/mg protein,
P
= 0.000; 210.28 ± 0.98 vs. 126.4 ± 0.13 pg/mL,
P
= 0.000, respectively) and decreased in the genistein group when compared to the IMN group (2.87 ± 0.37 vs. 9.70 ± 0.40 nmol/mg protein,
P
= 0.000; 156.59 ± 0.10 vs. 210.28 ± 0.98 pg/mL,
P
= 0.000, respectively). Serum PGE
2
level in IMN group was decreased significantly compared with control group (152.83 ± 0.10
vs
. 303.33 ± 2.16 pg/mL,
P
= 0.000) and increased in the genistein group compared to the IMN group (247.65 ± 0.01
vs
. 152.83 ± 0.10 pg/mL,
P
= 0.000). Expression of tissue iNOS was increased in the IMN group and improved in genistein groups. Most of the rats in the IMN group developed moderate to severe gastric erosion and ulcers. Gastric erosions and neutrophil infiltration score were significantly decreased in the genistein group.
Conclusions:
Genistein attenuated IMN-induced gastropathy in rats by reducing inflammation, decreasing oxidative stress, restoring mucoprotective function, and improving gastric histopathology.
Abbreviations used:
NSAIDs: Non-steroidal anti-inflammatory drugs; IMN: Indomethacin; COX: Cyclooxygenase; TNF: Tumor necrosis factor; ICAM: Intercellular adhesion molecule; iNOS: Inducible nitric oxide synthase; MDA: Malondialdehyde; CINC: Cytokine-induced neutrophil chemoattractant.
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Mechanism of action of isolated caffeic acid and epicatechin 3-gallate from
Euphorbia hirta
against
Pseudomonas aeruginosa
p. 311
Shanmugapriya Perumal, Roziahanim Mahmud, Sabariah Ismail
DOI
:10.4103/pm.pm_309_15
PMID
:28808398
Background:
The escalating dominance of resistant
Pseudomonas aeruginosa
strains as infectious pathogen had urged the researchers to look for alternative and complementary drugs.
Objective:
The objective of this study is to address the biological targets and probable mechanisms of action underlying the potent antibacterial effect of the isolated compounds from
Euphorbia hirta
(L.) against
P. aeruginosa.
Materials and Methods:
The action mechanisms of caffeic acid (CA) and epicatechin 3-gallate (ECG) on
P. aeruginosa
cells were investigated by several bacterial physiological manifestations involving outer membrane permeabilization, intracellular potassium ion efflux, and nucleotide leakage.
Results:
The findings revealed that ECG and CA targeted both cell wall and cytoplasmic membrane of
P. aeruginosa
. The cellular membrane destruction and ensuing membrane permeability perturbation of
P. aeruginosa
had led to the ascending access of hydrophobic antibiotics, release of potassium ions, and leakages of nucleotides.
Conclusion:
The overall study concludes that ECG and CA isolated from
E. hirta
possess remarkable anti-infective potentials which can be exploited as drug template for the development of new antibacterial agent against resistant
P. aeruginosa
pathogen.
Abbreviations used:
ECG: Epicatechin 3-gallate; CA: Caffeic acid;
E. hirta
:
Euphoria hirta
.
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Wound healing activity of
Azadirachta indica
A. juss stem bark in mice
p. 316
Preeti Maan, Kuldeep Singh Yadav, Narayan Prasad Yadav
DOI
:10.4103/0973-1296.210163
PMID
:28808399
Background:
The paste of stem bark of
Azadirachta indica
(AI) has been traditionally used on wound and scar for rapid healing in Bundelkhand region of India.
Objective:
In the present investigation, wound healing potential of different extracts of stem bark of AI was explored in mice model.
Materials and Methods:
To study the wound healing properties in small animal model, the excision and incision wound models were used and water, ethanol-water (1:1, v/v) and ethanol extracts were applied topically (15% w/w in ointment base). In the excision wound model, wound contraction, hydroxyproline content, DNA content, protein content, and nitric oxide levels were estimated after 14 days of topical treatment along with histopathological examinations. In the incision wound model, wound breaking strength was determined after 10 days of topical application of different extracts of AI.
Results:
The animals treated with water extract of AI exhibited significant increment in rate of wound contraction (93.39%,
P
< 0.01), hydroxyproline content (13.31 ± 6.65 mg/g of dry tissue,
P
< 0.001), DNA content (20.99 ± 0.68 μg/100 mg of tissue,
P
< 0.01), protein content (100.53 ± 7.88 mg/g of wet tissue,
P
< 0.01) and nitric oxide level (3.05 ± 0.03 mMol/g of tissue,
P
< 0.001) as well as in wound breaking strength (289.40 ± 29.45 g,
P
< 0.01) when compared with vehicle control group which was also supported by histopathological studies.
Conclusion:
The water extract of stem bark of AI possesses significant wound healing property, validating its traditional use.
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Induction of apoptosis and cell cycle arrest by flavokawain C on HT-29 human colon adenocarcinoma via enhancement of reactive oxygen species generation, upregulation of p21, p27, and Gadd153, and inactivation of inhibitor of apoptosis proteins
p. 321
Chung-Weng Phang, Saiful Anuar Karsani, Sri Nurestri Abd Malek
DOI
:10.4103/0973-1296.210180
PMID
:28808400
Chalcones have been shown to exhibit anti-cancer properties by targeting multiple molecular pathways. It was, therefore, of interest to investigate flavokawain C (FKC), a naturally occurring chalcone, which can be isolated from Kava (
Piper methysticum
Forst) root extract. The aim of this study was to investigate the inhibitory effect of FKC on the growth of HT-29 cells and its underlying mechanism of action. Cell viability of HT-29 cells was assessed by Sulforhodamine B assay after FKC treatment. Induction of apoptosis was examined by established morphological and biochemical assays. ROS generation was determined by dichlorofluorescein fluorescence staining, and superoxide dismutase activity was measured using the spectrophotometric method. Western blotting was used to examine the changes in the protein levels. FKC markedly decreased the cell viability of HT-29 cells and the cells showed dramatic changes in cellular and nuclear morphologies with typical apoptotic features. The induction of apoptosis correlated well with the externalization of phosphatidylserine, DNA fragmentation, decreased mitochondrial membrane potential, activation of caspases, and PARP cleavage. This was associated with an increase in reactive oxygen species and a decrease in SOD activity. The protein levels of XIAP, c-IAP1, and c-IAP2 were downregulated, whereas the GADD153 was upregulated after FKC treatment. FKC induced cell cycle arrest at the G
1
and G
2
/M phases via upregulation of p21 and p27 in a p53-independent manner. Our results provide evidence that FKC has the potential to be developed into chemotherapeutic drug for the treatment of colon adenocarcinoma.
Abbreviations used:
FKC: Flavokawain C; SRB: Sulforhodamine B; ROS: Reactive oxygen species; SOD: Superoxide dismutase; PARP: Poly(ADP-ribose) polymerase ; ER: Endoplasmic reticulum; IAPs: Inhibitor of apoptosis proteins; TUNEL: Transferase dUTP nick end labeling; Annexin V-FITC: Annexin V conjugated with fluorescein isothicyanate
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In vitro
α-amylase and α-glucosidase inhibitory and cytotoxic activities of extracts from
Cissus cornifolia
planch parts
p. 329
Talent Chipiti, Mohammed Auwal Ibrahim, Moganavelli Singh, Md. Shahidul Islam
DOI
:10.4103/pm.pm_223_16
PMID
:28808401
Context:
Hyperglycemia is the hallmark of type 2 diabetes mellitus, and its prevention will go a long way in managing the disease and its associated complications. Reduction of postprandial hyperglycemia through retarding carbohydrates digesting enzymes is one of the major therapeutic approaches used in the management of diabetes.
Objective:
The aim of the present study was to investigate the antidiabetic and cytotoxic effects of
Cissus cornifolia
extracts
in vitro
.
Materials and Methods:
The α-amylase and α-glucosidase inhibitory activities of ethanolic and aqueous extracts of
C. cornifolia
root and leaves were investigated when the cytotoxic effects of these extracts were analyzed using MTT assay on human embryonic kidney (HEK 293) cell lines.
Results:
The root ethanolic extract showed a mild α-amylase inhibitory activity with IC
50
value of 22.75 ± 1.23 μg/ml, but strong α-glucosidase inhibitory activity with IC
50
value 2.81 ± 0.97 μg/ml and the aqueous root extract indicated moderate inhibition for both α-amylase and α-glucosidase with IC
50
values of 33.70 ± 3.75 and 37.48 ± 2.35 μg/ml, respectively. The ethanolic root extract was found nontoxic at tested concentrations on HEK 293 cell lines as confirmed by the MTT assay with 93% cell viability at the highest concentration (200 μg/ml) tested. However, the aqueous extracts (leaf and root) were found cytotoxic at concentrations above 50 μg/ml.
Conclusion:
Data of this study suggest that the root ethanolic extracts of
C. cornifolia
possesses moderate α-amylase, but strong α-glucosidase inhibitory activity
in vitro
and did not show significant cytotoxicity with the tested concentrations.
Abbreviations used:
alex: Aqueous leaf extract; arex: Aqueous root extract; CC:
Cissus cornifolia
; DNS: Dinitrosalicylic acid; DMSO: Dimethylsulfoxide; elex: Ethanolic leaf extract; erex: Ethanolic root extract; IDF: International Diabetes Federation; MEM: Minimum essential medium; NIDDM: Noninsulin-dependent diabetes mellitus; pNPG: Para-nitrophenyl glucopyranoside; SD: Standard deviation; T2D: Type 2 diabetes.
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Role of
Adiantum philippense
L. on glucose uptake in isolated pancreatic cells and inhibition of adipocyte differentiation in 3T3-L1 cell line
p. 334
Tania Paul, Kishori G Apte, Pradeep B Parab, Biswadeep Das
DOI
:10.4103/pm.pm_415_16
PMID
:28808402
Background:
Adiantum philippense
(AP) is a pteridophyte that shows antihyperglycemic activity
in vivo
diabetic model, but the mechanism of action is unknown.
Objective:
AP was found to play a pivotal role in minimizing the high blood glucose in alloxan-induced diabetic rats. Simultaneously, it was observed that it could maintain the normal lipid profile even in diabetic condition. To investigate its insulin-like activity along with its inhibitory role on adipocyte differentiation became the objective of our present study.
Materials and Methods:
Glucose uptake potential of this fern was done in isolated pancreatic islets and inhibition of adipocyte differentiation was assessed in 3T3-L1 cell line. Before this, the cytotoxic concentration was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on L929 cell line. To determine its role in lipid metabolism, the oil droplets produced in adipocytes were stained with Oil 'O' red staining, and triglyceride levels of various drug treatments were measured spectrophotometrically.
Results:
This fern extract was found to be actively utilizing glucose in the glucose uptake assay. Moreover, it was also involved in inhibiting differentiation of pro-adipocyte to adipocyte in the 3T3-L1 cell lines. The percentage inhibition as obtained from the absorbance showed that the ethanolic extract at the concentration of 200 μg/ml showed 32.48% inhibition.
Conclusion:
All the above-mentioned parameters when appraised indicated that this fern could be used as an alternative medicine in managing diabetes associated with obesity.
Abbreviations used:
AP: Adiantum phillipense; MTT: (3-(4,5- Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide); BSA: Bovine serum albumin; FCS: Fetal calf serum; DMEM: Dulbecco's minimum essential media; RPMI: Roswell park memorial institute medium; DTZ: Dithizone; TG: Triglyceride; PPARγ: Peroxisome proliferator-activated receptor gamma; IBMX: 3-isobutyl-1-methylxanthine; nm: Nanometer; GI: Growth Inhibition; ELISA: Enzyme linked immunosorbent assay.
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The possible pre -and post -UVA radiation protective effect of amaranth oil on human skin fibroblast cells
p. 339
Katarzyna Wolosik, Ilona Zareba, Arkadiusz Surazynski, Agnieszka Markowska
DOI
:10.4103/pm.pm_522_15
PMID
:28808403
Background:
The health effects of Amaranth Oil (AO) are attributed to its specific chemical composition. That makes it an outstanding natural product for the prevention and treatment of ultraviolet (UV) irradiation-related pathologies such as sunburn, photoaging, photoimmunosuppression, and photocarcinogenesis. Most of the studies are taken on animal model, and there is a lack of research on the endogenous effect of AO on fibroblast level, where UVA takes it harmful place.
Objective:
The aim of this study was evaluation if AO can protect or abolish UVA exposure effect on human skin fibroblast.
Materials and Methods:
The 0.1% AO, 0.25% AO, and 0.5% AO concentration and irradiation for 15 min under UVA-emitting lamp were studied in various condition. In all experiments, the mean values for six assays ± standard deviations were calculated.
Results:
Pretreatment with various concentrations of AO was tested. The highest concentration of AO where cell survival was observed was 0.5%. Cytotoxicity assays provided evidence for pre- and post-UVA protective effect of 0.1% AO among three tested concentrations. The results also provide evidence that UVA has inhibitory effect on collagen biosynthesis in confluent skin fibroblast, but presence of 0.1% AO abolishes pre- and post-UVA effect comparing to other used AO concentration. The assessment results on DNA biosynthesis show the significant abolished post-UVA effect when 0.1% and 0.5% of AO were added.
Conclusion:
AO gives pre- and post-UVA protection in low concentration. This provides the evidence for using it not as a main protective factor against UV but as one of the combined components in cosmetic formulation.
Abbreviations used:
AO: Amaranth Oil.
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In vitro
antioxidant and antiproliferative activities of various solvent fractions from
Clerodendrum viscosum
leaves
p. 344
Anil Khushalrao Shendge, Tapasree Basu, Dipankar Chaudhuri, Sourav Panja, Nripendranath Mandal
DOI
:10.4103/pm.pm_395_16
PMID
:28808404
Background:
Free radicals such as reactive oxygen and nitrogen species, generated in the body, play an important role in the fulfillment of various physiological functions but their imbalance in the body lead to cellular injury and various clinical disorders such as cancer, neurodegenaration, and inflammation.
Objective:
The objective of this study is to fight this problem, natural antioxidant from plants can be considered as possible protective agents against various diseases such as cancer which might also modify the redox microenvironment to reduce the genetic instability. This study was designed to evaluate the antioxidant and antiproliferative potential of
Clerodendrum viscosum
fractions against various carcinomas.
Materials and Methods:
In this present study, 70% methanolic extract of
C. viscosum
leaves have been fractionated to obtain hexane, chloroform, ethyl acetate, butanol, and water fractions, which were tested for their antioxidant and anticancer properties.
Results:
It was observed that chloroform and ethyl acetate fractions showed good free radical scavenging properties as well as inhibited the proliferation of human lung cancer (A459), breast (MCF-7), and brain (U87) cells. Moreover, they arrested the cell cycle at G2/M phase of breast and brain cancer. These inhibitory effects were further confirmed by bromodeoxyuridine uptake imaging. Phytochemical investigations further indicate the presence of tannic acid, quercetin, ellagic caid, gallic acid, reserpine, and methyl gallate which might be the reason for these fractions' antioxidant and antiproliferative activities.
Conclusion:
Clerodendrum viscosum
leaf chloroform and
Clerodendrum viscosum
leaf ethyl acetate fractions from
C. viscosum
showed good reactive oxygen species and reactive nitrogen species scavenging potential. Both the fractions arrested cell cycle at G2/M phase in MCF-7 and U87 cells which lead to induce apoptosis.
Abbreviations used:
CVLME: Clerodendrum viscosum leaf methanolic extract; CVLH: Clerodendrum viscosum leaf hexane; CVLC: Clerodendrum viscosum leaf chloroform; CVLE: Clerodendrum viscosum leaf ethyl acetate; CVLB: Clerodendrum viscosum leaf butanol; CVLW: Clerodendrum viscosum leaf water; BrdU: Bromodeoxyuridine; WST-1: Water soluble tetrazolium salt.
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Catha edulis
Forsk. (Khat): Evaluation of its antidepressant-like Activity
p. 354
Hassan Alfaifi, Siddig Ibrahim Abdelwahab, Syam Mohan, Manal Mohamed Elhassan Taha, Sohier M Syame, Lamiaa A Shaala, Rashad Alsanosy
DOI
:10.4103/pm.pm_442_16
PMID
:28808405
Background:
Catha edulis
Forsk. (Khat) is traditionally used for treating various disorders. Nevertheless there are no reports of any scientific assessment of its psychopharmacological properties.
Objective:
Therefore, the current study was designed to evaluate the antidepressant-like activity of Khat ethanolic extract using established animal models of depression and stress.
Materials and Methods:
Ninety healthy male albino mice were used in this study. Forced swim, tail suspension and head poking tests were utilized to evaluate the antidepressant-like activity of the ethanolic extract of Khat (100, 200 and 400 mg/kg, i.p.) and escitalopram (standard drug) which were administered 30 min prior to the tests. Phytochemical analysis of the standardized extract was conducted using liquid chromatography-mass spectroscopy (LC-MS).
Results:
A significant decrease in the head-dipping behavior was noticed after administration of 100, 200 and 400 mg/kg of Khat extract. Moreover, the extract significantly decreased the immobility time in tail suspension and forced swim tests. The presence of cathinone and cathine were detected in the extract using LC-MS.
Conclusion:
The current results suggest that the extract of Khat leaves has acute antidepressant properties and may have sedative effects.
Abbreviations used:
LC-MS: Liquid chromatography-mass spectroscopy; NIST: National institute of standard technology; SSRI: Serotonin reuptake inhibitors; FST: Forced swim test; TST: Tail suspension test.
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Peimine inhibits the production of proinflammatory cytokines through regulation of the phosphorylation of NF-κB and MAPKs in HMC-1 Cells
p. 359
Ji Hye Park, Bina Lee, Hyun Kab Kim, Eun-Young Kim, Jae-Hyun Kim, Ju-Hee Min, Sunkook Kim, Youngjoo Sohn, Hyuk-Sang Jung
DOI
:10.4103/0973-1296.210173
PMID
:28808406
Background:
Peimine is a major biologically active component of
Fritillaria ussuriensis.
Peimine was investigated in chronic inflammation response, but it has not been studied in mast cell-related immediate allergic reaction. The present study aimed to evaluate anti-allergic effect of peimine in human mast cell (HMC-1).
Materials and Methods:
The effect of peimine on cell viability was measured by MTS assay in HMC-1. Histamine release was investigated in rat peritoneal mast cells (RPMCs). Interleukin (IL)-6, IL-8, and tumor necrosis factor-α (TNF-α) expressions were measured by ELISA assay and reverse transcription-polymerase chain reaction. Mitogen-activated protein kinases (MAPKs) and nuclear factor-kappaB (NF-κB) were examined by Western blot. Passive cutaneous anaphylaxis (PCA) reactions were evaluated using Sprague-Dawley (SD) rats.
Results:
Peimine inhibited the production of pro-inflammatory cytokines, such as IL-6, IL-8, and TNF-α. Moreover, peimine reduced MAPKs phosphorylation and the nuclear NF-κB expression in PMACI-induced HMC-1. Peimine decreased PCA reactions in rats as well.
Conclusion:
Our study proved that peimine might be suitable for the treatment of mast cell-derived allergic inflammatory reactions.
Abbreviations used:
HMC-1: Human mast cell, MTS: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, RPMCs: Rat peritoneal mast cells. IL-6: Interleukin 6, IL-8: Interleukin 8, TNF-α: Tumor necrosis factor-α, MAPKs: Mitogen-activated protein kinases; NF-κB: Nuclear factor-kappaB, PCA: Passive cutaneous anaphylaxis reactions, SD: Sprague-Dawley.
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In vitro
cell viability by CellProfiler
®
software as equivalent to MTT assay
p. 365
Luciana S Gasparini, Nayana D Macedo, Elisângela F Pimentel, Marcio Fronza, Valdemar L Junior, Warley S Borges, Eduardo R Cole, Tadeu U Andrade, Denise C Endringer, Dominik Lenz
DOI
:10.4103/0973-1296.210176
PMID
:28808407
Objective:
This study evaluated
in vitro
cell viability by the colorimetric MTT stands for 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay compared to image analysis by CellProfiler
®
software.
Materials and Methods:
Hepatoma (Hepa-1c1c7) and fibroblast (L929) cells were exposed to isolated substances, camptothecin, lycorine, tazettine, albomaculine, 3-epimacronine, trispheridine, galanthine and
Padina gymnospora
,
Sargassum
sp. methanolic extract, and
Habranthus itaobinus
Ravenna ethyl acetate in different concentrations. After MTT assay, cells were stained with Panotic dye kit. Cell images were obtained with an inverted microscope equipped with a digital camera. The images were analyzed by CellProfiler
®
.
Results:
No cytotoxicity at the highest concentration analyzed for 3-epimacronine, albomaculine, galanthine, trispheridine,
P. gymnospora
extract and
Sargassum
sp. extract where detected. Tazettine offered cytotoxicity only against the Hepa1c1c7 cell line. Lycorine, camptothecin, and
H. itaobinus
extract exhibited cytotoxic effects in both cell lines. The viability methods tested were correlated demonstrated by Bland–Atman test with normal distribution with mean difference between the two methods close to zero, bias value 3.0263. The error was within the limits of the confidence intervals and these values had a narrow difference. The correlation between the two methods was demonstrated by the linear regression plotted as R
2
.
Conclusion:
CellProfiler
®
image analysis presented similar results to the MTT assay in the identification of viable cells, and image analysis may assist part of biological analysis procedures. The presented methodology is inexpensive and reproducible.
Abbreviations:
HPLC: High pressure liquid chromatography MTT: (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide)
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Anti-lipid potential of
Drimys brasiliensis
p. 370
Josueli Merotto, Larissa Vivan Cestonaro, Luciana Grazziotin Rossato-Grando, Luciano de Oliveira Siqueira, Charise Dallazem Bertol
DOI
:10.4103/pm.pm_306_16
PMID
:28808408
Background:
The traditional use of
Drimys brasiliensis
Miers (Winteraceae) in the south of Brazil to reduce cholesterol has not been described in scientific literature.
Objective:
To verify the hypocholesterolemic effects of
D. brasiliensis
using rats as animal model.
Materials and Methods:
The bark of
D. brasiliensis
was extracted with water with further lyophilization and was subjected to phytochemical analysis by high-performance liquid chromatography (HPLC), and free radical scavenging activities by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay to determine antioxidant potential. The hypocholesterolemic activity was determined in male Wistar rats treated with 100 and 250 mg/kg/day extract concomitantly fed a hypercaloric diet, over 20 days (prevention assay). In the treatment assay, rats were fed a hypercaloric diet for 40 days and received the extract (100 mg/kg/day) from day 20.
Results:
In this research, we found that the extract of the bark of
D. brasiliensis
was able to reduce the triglycerides significantly and reduce total cholesterol at doses 100 and 250 mg/kg/day and both administration regimens (prevention and treatment) in rats treated with the extract and hypercaloric diet. The extract showed strong antioxidant properties (DPPH assay), probably responsible by hypocholesterolemic activity of the plant. By HPLC, we detected catechin (1.34%), epicatechin (3.48%), rutin (0.86%), caffeic acid (0.45%), and ferulic acid (0.84%) in
D. brasiliensis
extract.
Conclusions:
We confirm the popular use of the plant to reduce of cholesterol.
Abbreviations used:
HPLC: High-performance liquid chromatography; PDA: Photodiode array detector; RS: Reference substances; DPPH: 1,1-diphenyl-2-picrylhydrazyl; VCEAC: Vitamin C equivalent antioxidant capacity.
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Novel approaches to extraction methods in recovery of capsaicin from habanero pepper (CNPH 15.192)
p. 375
Frederico S Martins, Leonardo L Borges, Claudia S. C. Ribeiro, Francisco J. B. Reifschneider, Edemilson C Conceição
DOI
:10.4103/0973-1296.210127
PMID
:28808409
Introduction:
The objective of this study was to compare three capsaicin extraction methods: Shoxlet, Ultrasound-assisted Extraction (UAE), and Shaker-assisted Extraction (SAE) from Habanero pepper, CNPH 15.192.
Materials and Methods:
The different parameters evaluated were alcohol degree, time extraction, and solid–solvent ratio using response surface methodology (RSM).
Results:
The three parameters found significant (
p
< 0.05) were for UAE and solvent concentration and extraction time for SAE. The optimum conditions for the capsaicin UAE and SAE were similar 95% alcohol degree, 30 minutes and solid–liquid ratio 2 mg/mL. The Soxhlet increased the extraction in 10–25%; however, long extraction times (45 minutes) degraded 2% capsaicin.
Conclusion:
The extraction of capsaicin was influenced by extraction method and by the operating conditions chosen. The optimized conditions provided savings of time, solvent, and herbal material. Prudent choice of the extraction method is essential to ensure optimal yield of extract, thereby making the study relevant and the knowledge gained useful for further exploitation and application of this resource.
Abbreviations used:
Nomenclature UAE: Ultrasound-assisted Extraction; SAE: Shaker-assisted Extraction.
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The effects of Erzincan grape (
Vitis vinifera
spp.,
Cimin
) and benzothiazol on a
Caenorhabditis elegans
organism model
p. 380
Hulya Ozpinar, Necati Ozpinar, Savas Karakus
DOI
:10.4103/0973-1296.210164
PMID
:28808410
Background:
Grapes and their products are known to have been used for the treatment of diseases throughout history.
Objective:
It was aimed to investigate the effects of Erzincan Cimin grapes on an organism model of
Caenorhabditis elegans
N2 wild type and
C. elegans
BS913 strains with gonad cancer.
Materials and Methods:
The effects of methanol extracts of the skin and seeds of Erzincan Cimin grapes were examined separately on
C. elegans
N2 wild type and an effect was determined on lifespan. By applying GS-MS analysis, a potential agent substance was determined in the skin and seed methanol extracts. This substance was purchased and the effects of this substance were investigated on lifespan and fertility in
C. elegans
BS913 strains with gonad cancer. In addition, the effects on young subjects exposed to this agent substance in L1 form were investigated.
Results:
Grape seed and skin methanol extract was observed to prolong the lifespan most at a dose of 10 mg/100 mL. Lifespan was determined to be at a maximum in a gonad cancer organism model with benzothiazol at a dose of 50 ppm. At the same dose, positive effects were determined on the fertility of strains with cancer. When the effects of benzothiazol were examined on young L1 forms, an evident retardation of growth was determined at doses of 10, 50, and 100 ppm.
Conclusion:
Owing to anti-carcinogenic effects of benzothiazol and benzothiazol-derived substances, they can be considered as agent substances in academic studies related to cancer.
Abbreviations used:
GC-MS: gas chromatography and mass spectrometry;
C. elegans
:
Caenorhabditis elegans
; NGM: Nematode growth medium;
E. coli
:
Escherichia coli
; FUDR: Fluorodeoxyuridine; LDL: Low-density lipoprotein.
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© Pharmacognosy Magazine | Published by Wolters Kluwer -
Medknow
Online since 1
st
Jan 2005 | New site online since 1
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Jan, 2010