Home
|
About PM
|
Editorial board
|
Search
|
Ahead of print
|
Current Issue
|
Archives
|
Instructions
|
Subscribe
|
Advertise
|
Contact us
|
Login
Search Article
Advanced search
Users Online: 544
Export selected to
Endnote
Reference Manager
Procite
Medlars Format
RefWorks Format
BibTex Format
Table of Contents
August 2014
Volume 10 | Issue 39 (Supplement)
Page Nos. 443-667
Online since Saturday, August 30, 2014
Accessed 90,210 times.
PDF access policy
Journal allows immediate open access to content in HTML + PDF
EPub access policy
Full text in EPub is free except for the current issue. Access to the latest issue is reserved only for the paid subscribers.
View issue as eBook
Author Institution Mapping
Issue citations
Issue statistics
RSS
Show all abstracts
Show selected abstracts
Export selected to
Add to my list
ORIGINAL ARTICLES
High performance liquid chromatography profiling of health-promoting phytochemicals and evaluation of antioxidant, anti-lipoxygenase, iron chelating and anti-glucosidase activities of wetland macrophytes
p. 443
Keng-Fei Ooh, Hean-Chooi Ong, Fai-Chu Wong, Nam-Weng Sit, Tsun-Thai Chai
DOI
:10.4103/0973-1296.139767
PMID
:25298659
Background:
The phytochemistry and bioactivity of wetland macrophytes are underexplored. Plants are known as the natural sources of phytochemical beneficial to health.
Objective:
The objective of this study is to analyze the phytochemical profiles and bioactivities of 10 extracts prepared from different plant parts of wetland macrophytes
Hanguana
malayana
,
Ludwigia
adscendens
and
Monochoria
hastata.
Materials
and
Methods:
High performance liquid chromatography (HPLC) was used to analyze the phytochemical profile of the extracts. Antioxidant assay such as 2,2-diphenyl-1-picrylhydrazyl, nitric oxide (NO) radical scavenging activity and ferric reducing antioxidant power were performed. Bioactivity assays carried out were anti-lipoxygenase, anti-glucosidase, and iron chelating.
Results:
Leaf extract of
L.
adscendens
had the highest 2,2-diphenyl-1-picrylhydrazyl (half of maximal effective concentration [EC
50
] =0.97 mg/mL) and NO (EC
50
= 0.31 mg/mL) scavenging activities. The extract also exhibited the highest iron chelating (EC
50
= 3.24 mg/mL) and anti-glucosidase (EC
50
= 27.5 μg/mL) activities. The anti-glucosidase activity of
L.
adscendens
leaf extract was comparable or superior to those of acarbose, myricetin and quercetin
.
Correlation between iron chelating and radical scavenging activities among the extracts implies the presence of dual-function phytoconstituents with concurrent iron chelating and radical scavenging activities. HPLC analysis revealed the presence of
p
-coumaric acid (
p-
CA), gallic acid (GA) and myricetin in all or most extracts.
M.
hastata
fruit and leaf extracts had the highest
p
-hydroxybenzoic acid content. Antioxidant and anti-glucosidase activities of the extracts were correlated with
p
-CA, GA, and myricetin contents.
Conclusion:
Our study demonstrated that wetland macrophytes
H.
malayana
,
L.
adscendens
and
M.
hastata
are potential sources of health-promoting phytochemicals with potent therapeutically-relevant bioactivities.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (3) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Chemical constituents and toxicological studies of leaves from
Mimosa caesalpiniifolia
Benth., a Brazilian honey plant
p. 456
Nayana Bruna Nery Monção, Luciana Muratori Costa, Daniel Dias Rufino Arcanjo, Bruno Quirino Araújo, Maria do Carmo Gomes Lustosa, Klinger Antônio da França Rodrigues, Fernando Aécio de Amorim Carvalho, Amilton Paulo Raposo Costa, Antônia Maria das Graças Lopes Citó
DOI
:10.4103/0973-1296.139773
PMID
:25298660
Background:
Mimosa caesalpiniifolia
Benth. (Leguminosae) is widely found in the Brazilian Northeast region and markedly contributes to production of pollen and honey, being considered an important honey plant in this region.
Objective:
To investigate the chemical composition of the ethanol extract of leaves from
M. caesalpiniifolia
by GC-MS after derivatization (silylation), as well as to evaluate the
in vitro
and
in vivo
toxicological effects and androgenic activity in rats.
Materials and Methods:
The ethanol extract of leaves from
Mimosa caesalpiniifolia
was submitted to derivatization by silylation and analyzed by gas chromatography-mass spectrometry (GC-MS) to identification of chemical constituents.
In vitro
toxicological evaluation was performed by MTT assay in murine macrophages and by
Artemia salina
lethality assay, and the
in vivo
acute oral toxicity and androgenic evaluation in rats.
Results:
Totally, 32 components were detected: Phytol-TMS (11.66%), lactic acid-2TMS (9.16%), α-tocopherol-TMS (7.34%) and β-sitosterol-TMS (6.80%) were the major constituents. At the concentrations analyzed, the ethanol extract showed low cytotoxicity against brine shrimp (
Artemia salina
) and murine macrophages. In addition, the extract did not exhibit any toxicological effect or androgenic activity in rats.
Conclusions:
The derivatization by silylation allowed a rapid identification of chemical compounds from the
M. caesalpiniifolia
leaves extract. Besides, this species presents a good safety profile as observed in toxicological studies, and possess a great potential in the production of herbal medicines or as for food consumption.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (7) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Anti-melanogenic activity of the novel herbal medicine, MA128, through inhibition of tyrosinase activity mediated by the p38 mitogen-activated protein kinases and protein kinase signaling pathway in B16F10 cells
p. 463
Aeyung Kim, Jin Yeul Ma
DOI
:10.4103/0973-1296.139774
PMID
:25298661
Background:
Recently, our research group developed MA128, a novel herbal medicine, and demonstrated that MA128 is effective for the treatment of asthma and atopic dermatitis (AD). In particular, postinflammatory hyper-pigmentation in AD mice was improved with MA128 treatment. Thus, in this study, we determined the effect of MA128 on melanogenesis and its underlying mechanism in murine B16F10 melanoma cells.
Materials
and
Methods:
After treatment with MA128 at 100 and 250 μg/mL and/or alpha-melanocyte stimulating hormone (α-MSH) (1 μM), cellular melanin content and tyrosinase activity in B16F10 cells were measured. Using western blotting, expression levels of tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, microphthalmia-associated transcription factor (MITF), and activation of c-AMP-dependent protein kinase (PKA), c-AMP-related element binding protein (CREB) and mitogen-activated protein kinases (MAPKs) were examined.
Results:
MA128 significantly inhibited melanin synthesis and tyrosinase activity in a resting state as well as α-MSH-stimulating condition, and significantly decreased the expression of tyrosinase, TRP-1, TRP-2 and MITF. In addition, phosphorylation of PKA and CREB by α-MSH stimulation was efficiently blocked by MA128 pretreatment. Moreover, MA128 as an herbal mixture showed synergistic anti-melanogenic effects compared with each single constituent herb.
Conclusion:
MA128 showed anti-melanogenic activity through inhibition of tyrosinase activity mediated by p38 MAPK and PKA signaling pathways in B16F10 cells. These results suggest that MA128 may be useful as an herbal medicine for controlling hyper-pigmentation and as a skin-whitening agent.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (7) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Ultra high performance liquid chromatography- ultraviolet-electrospray ionization-micrOTOF-Q II analysis of flavonoid fractions from
Jatropha tanjorensis
p. 472
Arun Kallur Purushothaman, Brindha Pemiah
DOI
:10.4103/0973-1296.139776
PMID
:25298662
Background:
Jatropha
tanjorensis
(
Euphorbiaceae
) an exotic traditional plant unique to Thanjavur district of Southern India also commonly called as Catholic vegetable. It has been used traditionally in decoctions for treating various ailments and as a health tonic.
Objective:
The objective of the present work is to study a comprehensive characterization of methanolic extract fractions using ultra high performance liquid chromatography (UHPLC)+-electrospray ionization (ESI)-micrOTOF-Q II and correlate their bioactivities.
Materials and Methods:
Phytoconstituents from
J.
tanjorensis
leaves were extracted with methanol (MeOH) followed by successive chromatography using linear gradient polar solvents system. All fractions obtained were evaluated for their chemical potential using micrOTOF-Q II techniques and identified key molecules were determined for their anticancer and anti-oxidant potential using
in vitro
methods.
Results:
Successive column chromatography of the MeOH residue yielded six fractions. Compounds such as such as C-glycosylflavones (mono-C-, di-C-), O, C-diglycosylflavones and aglycones were identified for the first time in this plant using UHPLC-ultraviolet-micrOTOF-Q II ESI and a correlation with their anticancer using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay on Ehrlich ascites cells (EAC) and antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl and lipid peroxidation were studied; fraction D extract exhibited the strongest activity against cancer cell.
Conclusions:
LC-mass spectrometry has been successfully applied for a quick separation and identification of the major phytoconstituents. All fractions have shown potent antioxidative activity as compared to standard antioxidant 3,5-di-tert-butyl-4-hydroxytoluene. EAC cell-based cytotoxicity assay also revealed encouraging results. The antioxidant and anticancer activity determined in the present work can be attributed to the presence of flavonoids and flavone glycosides. Present work provides the first scientific report on phytoconstituents of
J.
tanjorensis
and its ethnopharmacological significance.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (1) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Multiple shoot regeneration and effect of sugars on growth and nitidine accumulation in shoot cultures of
Toddalia asiatica
p. 480
Chinthala Praveena, Ciddi Veeresham
DOI
:10.4103/0973-1296.139777
PMID
:25298663
Background:
Toddalia asiatica
(
Rutaceae
) is an important medicinal plant in traditional medicinal system of India and China. Nitidine production from callus cultures of the plant had been investigated, but
in vitro
multiplication and secondary metabolite production from shoot cultures is not reported.
Objective:
The aim of the present work is to establish protocol for
in vitro
multiple shoot regeneration of
T. asiatica
and to investigate the secondary metabolite, nitidine production from the shoot cultures.
Materials and Methods:
Different explants were used for shoot regeneration on MS supplemented with benzyl adenine (BA) either alone or in combination with naphthalene acetic acid (NAA) in different combinations. Effect of different sugars and different concentrations of sucrose on biomass accumulation in shoot cultures in liquid medium was investigated. For
in vitro
rooting, shoots culture were inoculated to half strength MS medium supplemented with different concentrations of indole butyric acid. Quantitative analysis of shoot culture extracts was done for estimation of nitidine by HPTLC.
Results:
Shoot cultures were successfully initiated and established from nodal and shoot tip explants on MS medium supplemented with benzyl adenine and sucrose (3% w/v). Sucrose at a concentration of 3 % w/v was found to be optimum for growth and biomass accumulation.
In vitro
rooting of shoots was achieved on half strength MS medium supplemented with indole butyric acid 3 mg/l. Investigation of secondary metabolite production ability of the
in vitro
regenerated shoot cultures revealed their ability to biosynthesize nitidine.
Conclusion:
Shoot cultures were established and nitidine production has been observed.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (3) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Effects of rhamnocitrin 4-β-D-galactopyranoside, isolated from
Astragalus hamosus
on toxicity models
in vitro
p. 487
Magdalena Kondeva-Burdina, Ilina Krasteva, Mitka Mitcheva
DOI
:10.4103/0973-1296.139778
PMID
:25298664
Background:
Astragalus
hamosus
L. (Fabaceae) is used in herbal medicine as emollient, demulcent, phrodisiac, diuretic, laxative, and good for inflammation, ulcers, and leukoderma. It is useful in treating irritation of the mucous membranes, nervous affections, and catarrh.
Objective:
Rhamnocitrin 4-β-D-galactopyranoside (RGP), isolated from
A.
hamosus
, was investigated for its possible protective effect on different models of toxicity
in vitro
on sub-cellular and cellular level.
Materials
and
Methods:
The effects of RGP were evaluated on isolated rat brain synaptosomes, prepared by Percoll reagent and on rat hepatocytes, isolated by two-stepped collagenase perfusion.
Results:
In synaptosomes, RGP had statistically significant protective effect, similar to those of silymarin, on 6-hydroxy (OH)-dopamine-induced oxidative stress. These results correlate with literature data about protective effects of kempferol and rhamnocitrin on oxidative damage in rat pheochromocytoma PC12 cells. In rat hepatocytes, we investigate the effect of RGP on two models of liver toxicity: Bendamustine and cyclophosphamide. In these models, the compound had statistically significant cytoprotective and antioxidant activity, similar to those of silymarin.
Conclusion:
According to these results, we can suggest that such cytoprotective effect of RGP might be due to an influence on bendamustine and cyclophosphamide metabolism in rat hepatocytes. In isolated rat hepatocytes, in combination with bendamustine and cyclophosphamide and in 6-OH-dopamine-induced oxidative stress in isolated rat synaptosomes, RGP, isolated from
A.
hamosus
, was effective protector and antioxidant. The effects were closed to those of flavonoid silymarin-the classical hepatoprotector and antioxidant.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (6) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Inhibition of inducible nitric oxide synthase and osteoclastic differentiation by
Atractylodis Rhizoma Alba
extract
p. 494
Sung-Ho Choi, Sung-Jin Kim
DOI
:10.4103/0973-1296.139780
PMID
:25298665
Background:
Atractylodis Rhizoma Alba
(ARA) has been used in Korean folk medicine for constipation, dizziness, and anticancer agent. In the present study, we performed to test whether the methanolic extract of ARA has antioxidant and antiosteoclastogenesis activity in RAW 264.7 macrophage cells.
Materials and Methods:
Antioxidant capacities were tested by measuring free radical scavenging activity, nitric oxide (NO) levels, reducing power, and inducible nitric oxide synthase (iNOS) expression in response to lipopolysaccharides (LPS). Antiosteoclastogenesis activity was evaluated by performing tartrate-resistant acid phosphatase assay in RAW 264.7 macrophage cells.
Results:
The extract exerted significant 1,1-diphenyl-2-picrylhydrazyl and NO radical scavenging activity, and it exerted dramatic reducing power. Induction of iNOS and NO by LPS in RAW 264.7 cells was significantly inhibited by the extract, suggesting that the ARA extract inhibits NO production by suppressing iNOS expression. Strikingly, the ARA extracts substantially inhibited the receptor activator of NF-κB ligand-induced osteclastic differentiation of LPS-activated RAW 264.7 cells. The ARA extract contains a significant amount of antioxidant components, including phenolics, flavonoids and anthocyanins.
Conclusion:
These results suggest that the methanolic extract of ARA exerts significant antioxidant activities potentially via inhibiting free radicals and iNOS induction, thereby leading to the inhibition of osteoclastogenesis.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (3) ]
[PubMed]
[Sword Plugin for Repository]
Beta
New steroidal saponin from
Antigonon leptopus
Hook. and
Arn.
p. 501
Maria Karmella L Apaya, Christine L Chichioco-Hernandez
DOI
:10.4103/0973-1296.139781
PMID
:25298666
Background:
Antigonon leptopus Hook. and Arn., Polygonaceae (cadena de amor), is a herbal remedy for pain and gout-like symptoms in the Philippines. The methanol extract of A. leptopus have shown strong inhibitory action against xanthine oxidase.
Objective:
To isolate and identify the compound responsible for the xanthine oxidase inhibitory action.
Materials and Methods:
A bioassay-guided isolation scheme using an in vitro assay for the inhibition of xanthine oxidase was employed. The structure was established using spectroscopic analysis and chemical methods.
Results:
The isolated compound was determined to be a noncompetitive inhibitor of xanthine with an IC50 of 1.79 μg/mL.
Conclusion:
The isolated compound may represent a new class of xanthine oxidase inhibitors.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (1) ]
[PubMed]
[Sword Plugin for Repository]
Beta
So-Cheong-Ryong-Tang, a herbal medicine, modulates inflammatory cell infiltration and prevents airway remodeling via regulation of interleukin-17 and GM-CSF in allergic asthma in mice
p. 506
Hyung-Woo Kim, Chi-Yeon Lim, Bu-Yeo Kim, Su-In Cho
DOI
:10.4103/0973-1296.139784
PMID
:25298667
Background:
So-Cheong-Ryong-Tang (SCRT), herbal medicine, has been used for the control of respiratory disease in East Asian countries. However, its therapeutic mechanisms, especially an inhibitory effect on inflammatory cell infiltration and airway remodeling in allergic asthma are unclear.
Objective:
The present study investigated the mechanism of antiasthmatic effects of SCRT in allergic asthma in mice.
Materials
and
Methods:
We investigated the influence of SCRT on levels of interleukin-17 (IL-17), granulocyte/macrophage colony-stimulating factor (GM-CSF), IL-4, and interferon gamma (IFN-γ) in bronchoalveolar lavage fluid (BALF), ovalbumin (OVA)-specific IgE in serum, and histopathological changes in allergen-induced asthma.
Results:
So-Cheong-Ryong-Tang decreased levels of IL-17 and GM-CSF in BALF. IL-4, a Th2-driven cytokine, was also decreased by SCRT, but IFN-γ, a Th1-driven cytokine, was not changed. Levels of OVA-specific IgE in serum were also decreased by SCRT. With SCRT treatment, histopathological findings showed reduced tendency of inflammatory cell infiltration, and prevention from airway remodeling such as epithelial hyperplasia.
Conclusion:
In this study, we firstly demonstrated that regulation of IL-17 and GM-CSF production may be one of the mechanism contributed to a reduction of inflammatory cell infiltration and prevention from airway remodeling.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (2) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Efficacy evaluation of
Bauhinia variegata
L. stem bark powder as adjunct therapy in chronic
Staphylococcus aureus
mastitis in goat
p. 512
Jeevan Ranjan Dash, Tapas Kumar Sar, Indranil Samanta, Subodh Pal, Madhuchhanda Khan, Nimai Chand Patra, Uttam Sarkar, Asit Kumar Maji, Tapan Kumar Mandal
DOI
:10.4103/0973-1296.139786
PMID
:25298668
Objective:
The objective was to study the effect of
Bauhinia
variegata
L. stem bark powder as adjunct therapy in chronic
Staphylococcus aureus
mastitis in goat.
Materials
and
Methods:
Mastitis was induced by intracisternal inoculation of coagulase positive
S.
aureus
(J638) at the concentration of 2000 colony forming units. Group I animals were treated with repeated dose of ceftriaxone at 20 mg/kg intravenously, and Group II animals were treated with once daily oral administration of
B.
variegata
L. stem bark powder at 6 g/kg for 7 days followed by maintenance dose at 3 g/kg for next 7 days along with repeated dose of the antibiotic at 20 mg/kg intravenously at 4 days interval.
Results:
No significant improvement in the clinical condition of the udder was noticed in the group treated with repeated dose of ceftriaxone alone. However, in the group treated with
B.
variegata
L. stem bark powder along with repeated dose of ceftriaxone, no
S.
aureus
colony was seen at 96 h and onwards in milk samples with a marked decrease in somatic cell count and milk alkaline phosphatase activity and increased lactoperoxidase activity. Further, plasma and milk concentration of ceftriaxone/ceftizoxime was increased, which indicated antibacterial, bioenhancing and antiinflammatory properties of the bark powder. The Group II animals also exhibited marked reduction in polymorphonuclear cells and fibrous tissue indicating antifibrotic property of
B.
variegata
L.
Conclusion:
B.
variegata
L. stem bark powder can be considered as an effective adjunct therapy to intravenous ceftriaxone in
S.
aureus
chronic mastitis in goat.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (1) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Anticancer activity of flavane gallates isolated from
Plicosepalus curviflorus
p. 519
Ghada Ahmed Fawzy, Areej Mohammad Al-Taweel, Shagufta Perveen
DOI
:10.4103/0973-1296.139787
PMID
:25298669
Background:
Previous investigation of the methanol extract of
Plicosepalus curviflorus
leaves led to the isolation of two new flavane gallates (1, 2), together with other compounds including quercetin (3). The stems of
P. curviflorus
are used traditionally for the treatment of cancer in Yemen.
Objective:
The aim of this study was to evaluate the anticancer activity of the plant methanol extract as well as isolated compounds (1-3).
Materials and Methods:
The human cancer cell lines used were; MCF-7, HepG-2, HCT-116, Hep-2, HeLa and normal, Vero cell line using the Crystal Violet Staining method (CVS).
Results:
Quercetin (3) possessed the highest anticancer effect against all five cell lines (IC
50
ranging from 3.6 to 16.2 μg/ml). It was followed by
2S
,
3R
-3, 3′, 4′, 5, 7-pentahydroxyflavane-5-
O
-gallate
(1)
, with IC
50
ranging from 11.6 to 38.8 μg/ml. The weakest anticancer activity was given by
2S
,
3R
-3,3′,4′,5,5′,7-hexahydroxyflavane-3′,5-di-
O
-gallate (2) with IC
50
ranging from 39.8 to above 50 μg/ml, compared to vinblastine sulphate as reference drug. Colon, liver and breast cell lines seemed to be more sensitive to the tested compounds than the cervical and laryngeal cell lines. Concerning the cytotoxic effect on Vero cell line, the pentahydroxyflavane-5-
O
-gallate (1) showed the highest IC
50 (
138.2 μg/ml), while quercetin exhibited the lowest IC
50
to Vero cells (30.5 μg/ml), compared to vinblastine sulphate as reference drug (IC
50:
39.7 μg/ml).
Conclusion:
The results suggest the possible use of compounds 1 and 3 as anticancer drugs especially against colon and liver cancers.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (10) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Anticancer potential of
Conium maculatum
extract against cancer cells
in vitro
: Drug-DNA interaction and its ability to induce apoptosis through ROS generation
p. 524
Jesmin Mondal, Ashis Kumar Panigrahi, Anisur Rahman Khuda-Bukhsh
DOI
:10.4103/0973-1296.139792
PMID
:25298670
Objective:
Conium maculatum
extract is used as a traditional medicine for cervix carcinoma including homeopathy. However, no systematic work has so far been carried out to test its anti-cancer potential against cervix cancer cells
in vitro
. Thus, in this study, we investigated whether ethanolic extract of conium is capable of inducing cytotoxicity in different normal and cancer cell lines including an elaborate study in HeLa cells.
Materials and Methods:
Conium's effects on cell cycle, reactive oxygen species (ROS) accumulation, mitochondrial membrane potential (MMP) and apoptosis, if any, were analyzed through flow cytometry. Whether Conium could damage DNA and induce morphological changes were also determined microscopically. Expression of different proteins related to cell death and survival was critically studied by western blotting and ELISA methods. If Conium could interact directly with DNA was also determined by circular dichroism (CD) spectroscopy.
Results:
Conium treatment reduced cell viability and colony formation at 48 h and inhibited cell proliferation, arresting cell cycle at sub-G stage. Conium treatment lead to increased generation of reactive oxygen species (ROS) at 24 h, increase in MMP depolarization, morphological changes and DNA damage in HeLa cells along with externalization of phosphatidyl serine at 48 hours. While cytochrome c release and caspase-3 activation led HeLa cells toward apoptosis, down-regulation of Akt and NFkB inhibited cellular proliferation, indicating the signaling pathway to be mediated via the mitochondria-mediated caspase-3-dependent pathway. CD-spectroscopy revealed that Conium interacted with DNA molecule.
Conclusion:
Overall results validate anti-cancer potential of Conium and provide support for its use in traditional systems of medicine.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (6) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Euphorbia hirta
accelerates fibroblast proliferation and Smad-mediated collagen production in rat excision wound
p. 534
Aadesh Upadhyay, Pronobesh Chattopadhyay, Danswrang Goyary, Papiya M Mazumder, Vijay Veer
DOI
:10.4103/0973-1296.139801
PMID
:25298671
Background:
Euphorbia hirta
L. (Euphorbiaceae) is a traditional herbal medicine used for treatment of various diseases.
Objective:
E. hirta
was investigated for
in vitro
/
in vivo
wound healing activity using human dermal fibroblast cell line and Wistar rats.
Materials and Methods:
Petroleum ether, chloroform, methanol and water successive extracts of
E. hirta
leaves were evaluated for antioxidant, antimicrobial and fibroblast proliferation activities. Among different extracts, the promising methanol extract was screened for wound healing activity in Wistar rats, using gentamicin sulfate (0.01% w/w) as a reference. Wound contraction, hydroxyproline content and the protein expression of COL3A1, bFGF, Smad-2,-3,-4 and -7 were measured.
Results:
The
E. hirta
methanol extract showed a potent antimicrobial (MIC 0.250 mg/ml against
Escherichia coli
and
Klebsiella pneumoniae
, both), antioxidant activities (IC
50
= 10.57 μg/ml
,
2,2-diphenyl-1-picrylhydrazyl; 850.23 μg/ml
,
superoxide-anion radical scavenging activity and 23.63 mg gallic acid equivalent per gram extract) with significant fibroblast proliferating activity (112% at 12.5 μg/ml) as compared to other extracts.
In vivo
study also supported the wound healing potential of methanol extract, as evidenced by faster wound contraction, higher hydroxyproline (4.240 mg/100 mg tissue) and improved histopathology of granulation tissue as compared to control groups and gentamicin sulfate-treated ones. Western blot also revealed a significantly altered expression of Smad-mediated proteins resulting in collagen production.
Conclusion:
The study suggested that
E. hirta
accelerates the wound healing by augmenting the fibroblast proliferation and Smad-mediated collagen production in wound tissue.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (2) ]
[PubMed]
[Sword Plugin for Repository]
Beta
A new mulinane diterpenoid from the cushion shrub
Azorella compacta
growing in Perú
p. 543
Francisco Salgado, Carlos Areche, Beatriz Sepúlveda, Mario J Simirgiotis, Fátima Cáceres, Cristina Quispe, Lina Quispe, Teresa Cano
DOI
:10.4103/0973-1296.139807
PMID
:25298672
Background:
Azorella compacta
is a rare yellow-green compact resinous cushion shrub growing from the high Andes of southern Perϊ to northwestern Argentina, and which is a producer of biologically active and unique diterpenoids.
Objective:
This study investigated the secondary metabolites present in a Peruvian sample of
Azorella compacta
and the evaluation of gastroprotective activity of the isolated compounds in a gastric- induced ulcer model in mice.
Material and Methods:
Six secondary metabolites (diterpenoids 1-6) present in the dichloromethane (DCM) extract of
A. compacta
growing in Perϊ were isolated by a combination of Sephadex LH-20 permeation and silica gel chromatography and their chemical structures were elucidated by spectroscopic methods (NMR) and molecular modeling. The gastroprotective activity of the new compound 1 was evaluated on the HCl/EtOH-induced gastric lesion model in mice and compared to the activity showed by the known compounds.
Results:
A new mulinane diterpene along with five known diterpenoids have been isolated from a Peruvian sample of
A. compacta
and the gastroprotective results show that compound
1
is less active than the other known mulinane diterpenoids isolated.
Conclusions:
A. compacta
growing in Perϊ showed the presence of the new mulinane 1, which was poorly active in the HCl/EtOH-induced gastric lesion model in mice. Indeed, the activity was lower than other diterpenoids (2-6) showing an oxygenated function at C-16 or/and C-20, which confirm the role of an oxygenated group (OH or carboxylic acid) for the gastroprotective activity of mulinane compounds.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (3) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Effect of three fatty acids from the leaf extract of
Tiliacora triandra
on P-glycoprotein function in multidrug-resistant A549RT-eto cell line
p. 549
Chutima Kaewpiboon, Pakorn Winayanuwattikun, Tikamporn Yongvanich, Preecha Phuwapraisirisan, Wanchai Assavalapsakul
DOI
:10.4103/0973-1296.139779
PMID
:25298673
Background:
Cancer cells have the ability to develop resistance to chemotherapy drugs, which then leads to a reduced effectiveness and success of the treatment. Multidrug resistance (MDR) involves the resistance in the same cell/tissue to a diverse range of drugs of different structures. One of the characteristics of MDR is an overexpression of P-glycoprotein (P-gp), which causes the efflux of the accumulated drug out of the cell. The MDR human non-small cell lung carcinoma cell line with a high P-gp expression level (A549RT-eto) was used to investigate the bioactive compounds capable of reversing the etoposide resistance in this cell line.
Materials and Methods:
The leaves of
Tiliacora triandra
were sequentially extracted with hexane, dichloromethane, methanol and water. Only the hexane extract reduced the etoposide resistance of the A549RT-eto cell line, and was further fractionated by column chromatography using the TLC-pattern and the restoration of etoposide sensitivity as the selection criteria.
Results:
The obtained active fraction (F22) was found by nuclear magnetic resonance and gas chromatography-mass spectroscopy analyses to be comprised of a 49.5:19.6:30.9 (w/w/w) mixture of hexadecanoic: octadecanoic acid: (Z)-6-octadecenoic acids. This stoichiometric mixture was recreated using pure fatty acids (MSFA) and gave a similar sensitization to etoposide and enhanced the relative rate of rhodamine-123 accumulation to a similar extent as F22, supporting the action via reducing P-gp activity. In contrast, the fatty acids alone did not show this effect.
Conclusion:
This is the first report of the biological activity from the leaves of
T. triandra
as a potential source of a novel chemosensitizer.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (3) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Strophanthus hispidus
attenuates the Ischemia-Reperfusion induced myocardial Infarction and reduces mean arterial pressure in renal artery occlusion
p. 557
Rohit Gundamaraju, Ravi Chandra Vemuri, Rajeev K Singla, Rishya Manikam, A Ranga Rao, Shamala Devi Sekaran
DOI
:10.4103/0973-1296.139782
PMID
:25298674
Background:
The myocardium is generally injured in the case of reperfusion injury and arterial damage is caused by hypertension. In reference to these statements, the present study was focused. Cardiac glycosides were said to have protective effects against myocardial infarction and hypertension.
Strophanthus hispidus
was thus incorporated in the study.
Objective:
The prime objective of the study was to investigate the protective effects of
Strophanthus hispidus
against ischemia-reperfusion myocardial Infarction and renal artery occluded hypertension in rats.
Materials and Methods:
The animal model adopted was surgically-induced myocardial ischemia, performed by means of left anterior descending coronary artery occlusion (LAD) for 30 min followed by reperfusion for another 4 h. Infarct size was assessed by using the staining agent TTC (2,3,5-triphenyl tetrazolium chloride). Hypertension was induced by clamping the renal artery with renal bulldog clamp for 4 h.
Results:
The study was fruitful by the effect of
Strophanthus hispidus
on infarction size, which got reduced to 27.2 ± 0.5and 20.0 ± 0.2 by 500 mg/Kg and 1000 mg/Kg ethanolic extracts which was remarkably significant when compared with that of the control group 52.8 ± 4.6. The plant extract did reduce heart rate at various time intervals. There was also a protective effect in the case of mean arterial blood pressure were the 500 mg/Kg and 1000 mg/Kg of the plant extract did reduce the hypertension after 60 minutes was 60.0 ± 4.80 and 50.50 ± 6.80.
Conclusion:
The results suggest that 500 mg/Kg and 100 mg/Kg ethanolic extract of
Strophanthus hispidus
was found to possess significant cardiac protective and anti-hypertensive activity.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (2) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Hepatoprotective effects of
Limonium tetragonum
, edible medicinal halophyte growing near seashores
p. 563
Min Hye Yang, Na-Hyun Kim, Jeong-Doo Heo, Sang Hyun Sung, Eun Ju Jeong
DOI
:10.4103/0973-1296.139783
PMID
:25298675
Background
: During the process of hepatic fibrosis, the activation of hepatic stellate cells (HSCs) is responsible for the increased formation and reduced degradation of extracellular matrix in the liver. By employing the hepatic stellate cell line, HSC-T6, it was found that the methanol extract of
Limonium tetragonum
, a halophyte living in salt marsh near south and western seashores of Korea significantly inhibited the proliferation of HSC-T6 cells.
Objective
: In the present study, we attempted to investigate the antifibrotic effects of the mathanolic extract of
L. tetragonum
(MELT) in the activated HSC-T6 cells.
Materials and Methods
: The proliferation of HSC-T6 was stimulated by culturing environment or platelet-derived growth factor (PDGF-BB) insult, and then the inhibitory activities of MELT were measured.
Results
: It was found that MELT suppressed the proliferation of the activated HSC-T6 in concentration- and time-dependent manners. The increased collagen deposition in the activated HSC-T6 cells was also decreased by the treatment of MELT. The maximal dose of MELT, however, had little effect on primary cultured rat hepatocytes. Wlammatory cytokine, tumor necrosis factor alpha (TNF-α) produced by lipopolysaccharide-stimulated RAW264.7 macrophages was inhibited by MELT.
Conclusion
: Collectively, the above results demonstrated that MELT suppressed HSCs proliferation but not in hepatocytes, implying that
L. tetragonum
may be useful candidates for developing therapeutic agents for the prevention and treatment of hepatic fibrosis.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (6) ]
[PubMed]
[Sword Plugin for Repository]
Beta
The effects of extraction method on recovery rutin from
Calendula officinalis
L. (Asteraceae)
p. 569
Frederico Severino Martins, Edemilson Cardoso da Conceição, Elane Sousa Bandeira, José Carréra Silva, Roseane Maria Ribeiro Costa
DOI
:10.4103/0973-1296.139785
PMID
:25298676
Background:
Calendula
officinalis
L
.
(Asteraceae) is a Mediterranean specie, but in Europe and America it is cultivated for ornamental or medicinal purposes. This species is widely used for presenting activities, antiinflammatory antibacterial and antioxidant. However the therapeutic action is linked to the amount of assets of the extracted raw material. The extraction method of bioactive compounds is an important step in the manufacturing of herbal medicines, because secondary metabolites with therapeutic potential are usually found in small quantities in plant materials.
Objective:
Due the medical and commercial importance of
C.
officinalis
, this study aimed to evaluate the impact of the extraction method on the quality of herbal extract and optimize the extraction of rutin from
C.
officinalis.
Materials
and
Methods
: The extraction of rutin was performed by ultrasound and shaker and the optimized conditions were determined by response surface methodology.
Results:
The results of ultrasound extraction assisted (UEA) and maceration dynamic (MD) showed that rutin yield ranged from 0.218 to 2.28% (w/w) when extract by ultrasound and 0.1-1.44% by MD. The optimal extraction condition for rutin (2.48% to UEA or 1.46% to MD) from
C.
officinalis
by UEA or MD were a 19-22 min extraction, ethanol: water ratio of 35-40% and 0.05-0.056 mg/mL to raw material: solvent ratio.
Conclusion:
The UEA is more efficient to extraction rutin.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (1) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Chemical composition and hepatotoxic effect of
Geranium schiedeanum
in a thioacetamide-induced liver injury model
p. 574
Juan Gayosso-De-Lucio, Mirandeli Bautista, C Velazquez-González, M De la O Arciniega, JA Morales-González, Juana Benedí
DOI
:10.4103/0973-1296.139788
PMID
:25298677
One of the major components of some geraniums is geraniin, described by its discoverer as crystallizable tannin, well known as an excellent antioxidant, and also found in fruits such as pomegranate. Recently, natural antioxidants have attracted great attention from consumers over the world due to their lower toxicity than synthetics. But geraniin is not a stable compound, and also is difficult to obtain, that is why in the present study we obtained acetonylgeraniin from
Geranium schideanum
(Gs), a stable acetone condensate of geraniin. In the present study the effect of Gs acetone-water extract was studied in reference to postnecrotic liver regeneration induced by thioacetamide (TA) in rats. Two months male rats were pretreated with daily dose of Gs extract for 4 days (300 mg/kg) and the last day also were intraperitoneally injected with TA (6.6 mmol/kg). Samples of blood were obtained from rats at 0, 24, 48, 72 and 96 h following TA intoxication. The pre-treatment with the crude extract in the model of thioacetamide-induced hepatotoxicity in rats decreased and delayed liver injury by 66% at 24 h. This result suggests that Gs extract may be used as an alternative for reduction of liver damage. On the other hand, acute toxicity study revealed that the LD
50
value of the
Gs
extract is more than the dose 5000 mg/kg in rats, according to the Lorke method.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (1) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Efficacy of ellagic acid and sildenafil in diabetes-induced sexual dysfunction
p. 581
Sumanta Kumar Goswami, Manikanta Vishwanath, Suma Kallahalli Gangadarappa, Rema Razdan, Mohammed Naseeruddin Inamdar
DOI
:10.4103/0973-1296.139790
PMID
:25298678
Background:
Diabetes induced sexual dysfunction is a leading cause of male sexual disorder and an early indicator of cardiovascular complication. Reactive oxygen species generated in body during diabetes is a main causative factor for erectile dysfunction, a sexual dysfunction. Adjuvant antioxidant therapy along with phosphodiesterases type 5 enzyme inhibitor (PDE5i) is more effective than PDE5i alone.
Objective:
The aim of the study was to investigate efficacy of ellagic acid a known antioxidant and sildenafil in diabetes induced erectile dysfunction.
Materials and Methods:
Type 1 diabetes was induced in male rats and rats were treated with ellagic acid (50 mg/kg, p.o.) and a combination of ellagic acid (50 mg/kg, p.o.) and sildenafil (5 mg/kg, p.o.), a PDE5i for 28 days. Sexual function was observed in diabetic rat and compared with those of treatment group and normal rats. Effect of ellagic acid was studied on
advanced glycation end products
(AGE) and isolated rat corpus cavernosum in vitro.
Results:
Sexual function of diabetic rats was found to be reduced and ellegic acid treatment could preserve sexual function of diabetic rats to some extent. Ellagic acid + sildenafil treatment was more efficient in management of diabetes induced sexual dysfunction. Ellagic acid inhibited (AGE)
in vitro
implying its role in reducing oxidative stress in diabetes. The polyphenol could not increase sexual function in normal rats and relax isolated rat corpus cavernosum smooth muscle significantly.
Conclusion:
The study proves usefulness of adjuvant antioxidant therapy in the management of erectile dysfunction in diabetes.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (18) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Anti-inflammatory effect of
Artemisiae annuae
herba in lipopolysaccharide-stimulated RAW 264.7 Cells
p. 588
You-Chang Oh, Yun Hee Jeong, Taesoo Kim, Won-Kyung Cho, Jin Yeul Ma
DOI
:10.4103/0973-1296.139793
PMID
:25298679
Background:
Artemisiae annuae
herba (AAH) has been traditionally used as a drug for the treatment of malaria, heat stroke, bacterial infection, and fever in East-Asia. Although AAH has been used for the treatment of inflammation-related symptoms, the underlying mechanism of antiinflammatory activity of AAH is still unknown.
Objective:
We investigated whether AAH have an inhibitory effect on the production of pro-inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 macrophage cells.
Materials and Methods:
The investigation was forced on the inhibitory effect of AAH on the production of tumor necrosis factor (TNF)-α, interleukin (IL)-6, nitric oxide (NO), and inducible NO synthase (iNOS) in macrophages. Furthermore, we examined the effect of AAH on the activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) pathways.
Results:
We found that AAH suppresses NO production and TNF-α, IL-6, and iNOS gene expression. Moreover, AAH inhibited the nuclear translocation of p65 and IκBα degradation in NF-κB pathway and decreased the extracellular signal-regulated kinase, p38, c-Jun NH
2
-terminal kinase phosphorylation in MAPK signaling pathway.
Conclusions:
Consequently, these results indicate that AAH contains antiinflammatory activity and this effect is derived from the repression on the activation of NF-κB and MAPKs pathways. We first demonstrated that antiinflammatory effect of AAH and its underlying mechanism in macrophage cells.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (3) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Bioassay-guided isolation, identification and molecular ligand-target insight of lipoxygenase inhibitors from leaves of
Anisomeles malabarica
R.Br.
p. 596
A Sudha, P Srinivasan
DOI
:10.4103/0973-1296.139795
PMID
:25298680
Background:
Anisomeles
malabarica
R. Br. (
Lamiaceae
) is extensively used in traditional medicine in major parts of India for several medicinal purposes, including their use in rheumatism.
Materials and Methods:
The air-dried leaves of
A.
malabarica
were extracted with ethanol, defatted with n-hexane and then successively partitioned into chloroform and n-butanol fractions. Bioassay-guided fractionation and purification of chloroform fraction from
A.
malabarica
lead to the isolation of lipoxygenase (LOX) inhibitors. The structures of isolated compounds were elucidated by ultraviolet, infrared,
1
H nuclear magnetic resonance (NMR),
13
C NMR and mass spectrometry spectroscopic techniques and assessed further by
in vitro
soybean lipoxygenase (sLOX) assay. In addition, the enzyme type inhibition was evaluated through molecular docking technique as a part of computational study.
Results:
The bioactive compounds 3, 4 dihydroxy benzoic acid (1) and 4', 5, 7-trihydroxyflavone (2) were isolated from chloroform fraction of
A.
malabarica
, whose bioactivity was observed to be dose-dependent compared to n-butanol fraction. Among the compounds, 3, 4 dihydroxy benzoic acid showed significant sLOX inhibitory activity with 74.04% ±2.6% followed by 4', 5, 7-trihydroxyflavone (34.68% ±1.9%). The computational analysis of compounds showed their molecular interaction with important amino acid residues and nonheme iron atom in the catalytic site of LOX by enlightening their potential binding mode at molecular level.
Conclusions:
The LOX inhibitory constituents were identified from
A.
malabarica
by means of bioassay-guided fractionation process. The results derived from
in vitro
and computational experiments confirm the potential of the isolated compounds and provide additional evidence for its traditional use in inflammatory disorders.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (2) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Optimization of the extraction process for the seven bioactive compounds in Yukmijihwang-tang, an herbal formula, using response surface methodology
p. 606
Jung-Hoon Kim, Hyeun-Kyoo Shin, Chang-Seob Seo
DOI
:10.4103/0973-1296.139798
PMID
:25298681
Background:
Yukmijihwang-tang (YJT) contains multiple bioactive compounds. Heat-reflux extraction was employed and optimized for the extraction of the bioactive compounds in YJT.
Objective:
The determination of optimal conditions with maximum yields of bioactive compounds, gallic acid, 5-hydroxymethylfurfural, morroniside, loganin, paeoniflorin, benzoic acid and paeonol, in YJT.
Materials and Methods:
The extraction ratio (ratio of water to herbal formula), extraction time and extraction number were set as individual values and the yields of the seven compounds were the response values that were optimized with a Box-Behnken design.
Results:
The optimal conditions obtained from response surface methodology (RSM) were 1:11.99 for the extraction ratio, 94.53 min for the extraction time and 2.21 for the extraction number. Under the optimal conditions, the response value of the experiment closely agreed with the predicted response value.
Conclusions:
The result suggests that RSM is successfully applied for optimizing the extraction of the marker compounds in YJT.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (3) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Effect of whole plant of
Rostellularia diffusa
Willd. on experimental stress in mice
p. 614
Mandipati Nagasirisha, TS Mohamed Saleem
DOI
:10.4103/0973-1296.139799
PMID
:25298682
Background:
Rostellularia
diffusa
is an unexplored medicinal plant used as brain tonic in traditional medicine system.
Objective:
This study was designed to investigate the antioxidant and anti-stress potential of
R.
diffusa
by experimental animal models.
Materials and Methods:
The extracts of
R.
diffusa
were subjected to preliminary phytochemical screening and high performance thin layer chromatography (HPTLC) finger printing analysis. The antioxidant potential of the extracts was found by different
in vitro
models. The anti-stress activity was investigated by using acetic acid induced writhing test, swimming endurance test, and restraint stress in experimental mice. Serum parameters such as glucose, triglyceride and cholesterol, oxidative stress parameter thiobarbituric acid reactive substance, antioxidant parameters such as reduced glutathione, superoxide dismutase and catalase and organ weights were evaluated after restraint stress in mice. Diazepam was used as reference standard to compare the anti-stress activity of plant extract.
Results:
High performance thin layer chromatography finger printing analysis revealed the presence of flavone compounds in both extracts. The extracts also showed good antioxidant property in different
in vitro
antioxidant models. Administration of extracts of
R.
diffusa
decreased the number of wriths and immobility time when compared with control group in acetic acid-induced writhing test and swimming endurance test respectively in experimental mice. They also suppressed the restraint stress-induced alterations in serum parameters, oxidative stress, and antioxidant parameters in brain and also restored the organ weights in normal level.
Conclusion:
From these results, it has been concluded that the potential anti-stress activity of
R.
diffusa
is through its adaptogenic and antioxidant properties.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (1) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Signal transducer and activators of transcription 3 regulates cryptotanshinone-induced apoptosis in human mucoepidermoid carcinoma cells
p. 622
Hyun-Ju Yu, Chul Park, Sun-Ju Kim, Nam-Pyo Cho, Sung-Dae Cho
DOI
:10.4103/0973-1296.139802
PMID
:25298683
Background:
Cryptotanshinone (CT) is a biologically active compound from the root of
Salvia miltiorrhiza
that has been reported to induce apoptosis in various cancer cell lines; but, it has not yet been fully explored in human mucoepidermoid carcinoma (MEC).
Objective:
Here, we demonstrated the apoptotic effects and its related mechanism in MC-3 and YD-15 human MEC cell lines.
Materials
and
Methods:
The effects of CT on apoptotic activity were evaluated by cell proliferation assay, Western blotting, 4'-6-diamidino-2-phenylindole staining, reverse transcription-polymerase chain reaction, and luciferase assay.
Results:
Our data show that CT treatment of MC-3 cells results in anti-proliferative and apoptotic activities in MC-3 and it is accompanied by a decrease in phosphorylation and dimerization of signal transducer and activators of transcription 3 (STAT3). CT decreased the expression levels of myeloid cell leukemia-1 (Mcl-1) and surviving, whereas Bcl-xL expression was not changed. CT clearly regulates survivin protein at a transcriptional level and alters Mcl-1 through proteasome-dependent protein degradation. In addition, CT-induced apoptotic cell death in YD-15, another human MEC cell line, was associated with the inhibition of STAT3 phosphorylation.
Conclusion:
These data suggest that CT could be a good apoptotic inducer through modification of STAT3 signaling in human MEC cell lines.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (5) ]
[PubMed]
[Sword Plugin for Repository]
Beta
The
Pavonia xanthogloea
(Ekman, Malvaceae): Phenolic compounds quantification, anti-oxidant and cytotoxic effect on human lymphocytes cells
p. 630
Clarice Pinheiro Mostardeiro, Marco Aurélio Mostardeiro, Ademir Farias Morel, Raul Moreira Oliveira, Alencar Kolinski Machado, Pauline Ledur, Francine Carla Cadoná, Ubiratan Flores da Silva, Ivana Beatrice Mânica da Cruz
DOI
:10.4103/0973-1296.139804
PMID
:25298684
Introduction:
Pavonia
xanthogloea
is traditionally used as an antimicrobial and anti-tumour medicine in Southern Brazilian region. However, investigations about this species are still incipient.
Hypothesis Tested:
The study postulated that
P.
xanthologea
specie present some phenolic compound and present some biological properties as anti-oxidant and cytoprotective effect against oxidative stress.
Materials and Methods:
The content of eight phenolic molecules in the crude ethanolic extract of the aerial part of
P.
xanthogloea
and its five fractions (hexane, dichloromethane, ethyl-acetate, n-butanol, and water) was determined by heterotrophic plate count method. The anti-oxidant capacity of the extract and the fractions was determined by 1,1-diphenyl-2-picryl-hydrazyl assay. The potential anti-oxidant and cytoprotective effect was also analyzed in human lymphocyte culture treated with extract/fractions at different concentrations with and without oxidative stress generated by hydrogen peroxide (H
2
O
2
) and sodium nitroprusside (SNP) exposition.
Results:
Tiliroside was the molecule detected in all extract. Water and ethyl-acetate fractions showed the highest radical-scavenging activity. The crude extract, hexane, water, and n-butanol reversed the higher reactive oxygen specie levels generated by H
2
O
2
and SNP to levels similar to those observed in the control group. In addition, crude extract, hexane, ethyl-acetate and n-butanol did not caused cytotoxicity, whereas water fraction was cytotoxic at higher concentration tested here (300 μg/mL). The cytotoxicity reversion caused by SNP exposition was concentration-dependent of the extract and fractions. However, dichloromethane fraction increased cell mortality in all concentrations investigated and was not able to decrease cell death in the lymphocytes exposed to SNP.
Conclusion:
The results suggest potential medicine use of this species.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (1) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Identification of natural inhibitors against angiotensin I converting enzyme for cardiac safety using induced fit docking and MM-GBSA studies
p. 639
Balakrishnan Vijayakumar, Subramani Parasuraman, Ramasamy Raveendran, Devadasan Velmurugan
DOI
:10.4103/0973-1296.139809
PMID
:25298685
Background:
Cleistanthins A and B are isolated compounds from the leaves of
Cleistanthus collinus
Roxb (Euphorbiaceae). This plant is poisonous in nature which causes cardiovascular abnormalities such as hypotension, nonspecific ST-T changes and QTc prolongation. The biological activity predictions spectra of the compounds show the presence of antihypertensive, diuretic and antitumor activities.
Objective:
Objective of the present study was to determine the
in silico
molecular interaction of cleistanthins A and B with Angiotensin I- Converting Enzyme (ACE-I) using Induced Fit Docking (IFD) protocols.
Materials and Methods:
All the molecular modeling calculations like IFD docking, binding free energy calculation and ADME/Tox were carried out using Glide software (Schrφdinger LLC 2009, USA) in CentOS EL-5 workstation.
Results:
The IFD complexes showed favorable docking score, glide energy, glide emodel, hydrogen bond and hydrophobic interactions between the active site residues of ACE-I and the compounds. Binding free energy was calculated for the IFD complexes using Prime MM-GBSA method. The conformational changes induced by the inhibitor at the active site of ACE-I were observed based on changes of the back bone Cα atoms and side-chain chi (x) angles. The various physicochemical properties were calculated for these compounds. Both cleistanthins A and B showed better docking score, glide energy and glide emodel when compared to captopril inhibitor.
Conclusion:
These compounds have successively satisfied all the
in silico
parameters and seem to be potent inhibitors of ACE-I and potential candidates for hypertension.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (11) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Lactobacilli-fermented Hwangryunhaedoktang has enhanced anti-inflammatory effects mediated by the suppression of MAPK signaling pathway in LPS-stimulated RAW 264.7 cells
p. 645
You-Chang Oh, Yun Hee Jeong, Won-Kyung Cho, Kwang Jin Lee, Taesoo Kim, Jin Yeul Ma
DOI
:10.4103/0973-1296.139815
PMID
:25298686
Background:
Hwangryunhaedoktang (HR) has been traditionally used in oriental medicine as a drug for the treatment of melena, hemoptysis, and apoplexy.
Objective:
We investigated whether HR and lactobacilli-fermented HRs have an inhibitory effect on the production of proinflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells.
Materials and Methods:
The investigation was focused on whether HR and fermented HRs could inhibit the production of prostaglandin (PG)E
2,
nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-6, cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) and mitogen-activated protein kinases (MAPKs) in LPS-stimulated RAW 264.7 cells.
Results:
We found that HR weakly inhibited various inflammatory mediators induced by LPS. However, fermentation with lactobacilli significantly increased the inhibitory effect of HR on most of the inflammatory mediator expression. Furthermore, fermented HRs exerted a stronger inhibitory effect on MAPKs phosphorylation than that by non-fermented HR.
Conclusions:
These results suggest that lactobacilli-fermented HRs contains elevated potent anti-inflammatory activity that is mediated by inhibiting MAPKs pathway in macrophages.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (4) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Antinociceptive effect of
Encholirium spectabile
: A Bromeliaceae from the Brazilian caatinga biome
p. 655
Sarah Raquel Gomes de Lima-Saraiva, Juliane Cabral Silva, Carla Rodrigues Cardoso Branco, Alexsandro Branco, Elba Lúcia Cavalcanti Amorim, Jackson Roberto Guedes da Silva Almeida
DOI
:10.4103/0973-1296.139817
PMID
:25298687
Background:
Encholirium spectabile
is a species found in outcrops rocky throughout the Brazilian Caatinga.
Objective:
This study was carried out to evaluate the antinociceptive effects of ethanolic extract of the leaves from
E. spectabile
(Es-EtOH) in mice using chemical and thermal models of nociception.
Material and Methods:
HPLC was used to determine the fingerprint chromatogram. The Es-EtOH was examined for its antinociceptive activity at the doses of 100, 200 and 400 mg/kg intraperitoneal (i.p.). The evaluation of antinociceptive activity was carried out by the acetic acid-induced writhing, formalin and hot plate tests in mice. Rota-rod test was used for the evaluation of motor coordination.
Results:
In the acetic acid-induced writhing test, the Es-EtOH (100, 200 and 400 mg/kg, i.p.) reduced the number of writhings by 68.59, 79.33 and 65.28%, respectively. Additionally, Es-EtOH (100, 200 and 400 mg/kg, i.p.) decreased by 34.14, 52.61 and 60.97% the paw licking time in the first phase, as well as 89.56, 79.90 and 96.71% in the second phase of the formalin test, respectively. Es-EtOH also showed effect in the hot plate test, since increased the latency time at dose of 100 mg/kg after 60 minutes. In addition, Es-EtOH did not impair motor coordination. The presence of phenolic compounds in the extract was confirmed using HPLC. These results indicate that Es-EtOH has antinociceptive activity, probably of peripheral origin. The mechanism involved is not completely understood but, at least in part there is the participation of opioid receptors.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (1) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Anti-cancer effects of
Kochia scoparia
fruit in human breast cancer cells
p. 661
Hye-Yeon Han, Hyungwoo Kim, Yong Hae Son, Guemsan Lee, Sung-Hee Jeong, Mi Heon Ryu
DOI
:10.4103/0973-1296.139812
PMID
:25298688
Background:
The fruit of
Kochia scoparia
Scharder is widely used as a medicinal ingredient for the treatment of dysuria and skin diseases in China, Japan and Korea. Especially,
K. scoparia
had been used for breast masses and chest and flank pain.
Objective:
To investigate the anti-cancer effect of
K. scoparia
on breast cancer.
Materials and Methods:
We investigated the anti-cancer effects of
K. scoparia
, methanol extract (MEKS)
in vitro
. We examined the effects of MEKS on the proliferation rate, cell cycle arrest, reactive oxygen species (ROS) generation and activation of apoptosis-associated proteins in MDA-MB-231, human breast cancer cells.
Results:
MTT assay results demonstrated that MEKS decreased the proliferation rates of MDA-MB-231 cells in a dose-dependent manner with an IC
50
value of 36.2 μg/ml. MEKS at 25 μg/ml significantly increased the sub-G1 DNA contents of MDA-MB-231 cells to 44.7%, versus untreated cells. In addition, MEKS induced apoptosis by increasing the levels of apoptosis-associated proteins such as cleaved caspase 3, cleaved caspase 8, cleaved caspase 9 and cleaved Poly (ADP-ribose) polymerase (PARP).
Conclusion:
These results suggest that MEKS inhibits cell proliferation and induces apoptosis in breast cancer cells and that MEKS may have potential chemotherapeutic value for the treatment of human breast cancer.
[ABSTRACT]
[HTML Full text]
[PDF]
[Mobile Full text]
[EPub]
[Citations (2) ]
[PubMed]
[Sword Plugin for Repository]
Beta
Feedback
Subscribe
Next Issue
Previous Issue
Sitemap
|
What's New
|
Feedback
|
Disclaimer
|
Privacy Notice
© Pharmacognosy Magazine | Published by Wolters Kluwer -
Medknow
Online since 1
st
Jan 2005 | New site online since 1
st
Jan, 2010