Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 17  |  Issue : 75  |  Page : 636--642

Metabolite profiling and in vitro evaluation of Lepisanthes fruticosa fruit pulp extract as inhibitor against dengue and West Nile virus NS2B-NS3 proteases


Suhaina Supian1, Machap Chandradevan1, Muhamad Aizuddin Ahmad1, Lina Rozano1, Mohd Shukri Mat Ali2, Sanimah Simoh1 
1 Biotechnology and Nanotechnology Research Centre, Malaysia Agricultural Research and Development Institute, Selangor, Malaysia
2 Horticulture Research Centre, Malaysia Agricultural Research and Development Institute, Selangor, Malaysia

Correspondence Address:
Sanimah Simoh
Biotechnology and Nanotechnology Research Centre, Malaysia Agricultural Research and Development Institute, Selangor
Malaysia

Background: Dengue virus serotype 2 (DENV2) and West Nile virus (WNV) fevers are mosquito-borne diseases with no effective treatment at present. In recent years, the development of plant-based antivirals targeting the viral NS2B-NS3 serine proteases has been the main focus as the synthetic antivirals available are not specific and less safe. Objectives: To evaluate the inhibitory activity of Lepisanthes fruticosa pulp extract against NS2B-NS3 proteases from DENV2 and WNV and identify the metabolites from this fruit extract. Materials and Methods: In vitro DENV2 and WNV NS2B-NS3 proteases assays were carried out using the methanolic extract of L. fruticosa pulp. Liquid chromatography-electron spray ionization-mass spectrometry/mass spectrometry (LC-ESI-MS/MS) and gas chromatography-mass spectrometry/mass spectrometry (GC-MS/MS) were performed to determine the metabolites present in this fruit species extract. Results: L. fruticosa extract exhibited inhibitory activity toward DENV2 and WNV NS2B-NS3 proteases with 50% inhibitory concentration value of 1.733 ± 0.195 and 9.245 ± 0.938 mg/mL, respectively. LC-ESI-MS/MS of L. fruticosa extract identified epigallocatechin-catechin, epigallocatechin, epicatechin, catechin, cyanidin rutinoside, procyanidin trimer, rutin, myricetin rhamnohexoside, luteolin glucoside and its derivative which were from the flavonoid group. In addition, GC-MS/MS identified fatty acids and sterols. Conclusion: The inhibitory activity of L. fruticosa pulp extract toward NS2B-NS3 proteases from DENV2 and WNV suggests this fruit species as a potential source for the development of antiviral. Metabolites from the groups of flavonols, flavones, and sterols identified in L. fruticosa pulp may contribute to the inhibitory properties of L. fruticosa.


How to cite this article:
Supian S, Chandradevan M, Ahmad MA, Rozano L, Mat Ali MS, Simoh S. Metabolite profiling and in vitro evaluation of Lepisanthes fruticosa fruit pulp extract as inhibitor against dengue and West Nile virus NS2B-NS3 proteases.Phcog Mag 2021;17:636-642


How to cite this URL:
Supian S, Chandradevan M, Ahmad MA, Rozano L, Mat Ali MS, Simoh S. Metabolite profiling and in vitro evaluation of Lepisanthes fruticosa fruit pulp extract as inhibitor against dengue and West Nile virus NS2B-NS3 proteases. Phcog Mag [serial online] 2021 [cited 2022 Jan 20 ];17:636-642
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2021;volume=17;issue=75;spage=636;epage=642;aulast=Supian;type=0