Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 16  |  Issue : 69  |  Page : 400--408

Clinacanthus nutans aqueous extract suppresses the release of histamine and β-Hexosaminidase inin-vitro model of IgE-mediated mast cell degranulation


Audrey Siew Foong Kow1, Lai Yen Fong1, Leng Wei Khoo2, Ji Wei Tan1, Ming-Tatt Lee3, Daud Ahmad Israf1, Faridah Abas4, Khozirah Shaari5, Chau Ling Tham1 
1 Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia
2 Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Malaysia
3 Faculty of Pharmaceutical Sciences, UCSI University, 56000 Kuala Lumpur, Malaysia; Graduate Institute of Brain and Mind Sciences; Graduate Institute of Pharmacology, College of Medicine, National Taiwan University, Taipei, Taiwan
4 Department of Food Science, Faculty of Food Science and Technology; Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Malaysia
5 Laboratory of Natural Products, Institute of Bioscience; Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Malaysia

Correspondence Address:
Chau Ling Tham
Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang Selangor
Malaysia

Introduction: Clinacanthus nutans (Burm. f.) Lindau, a shrub found in South East Asia, particularly in Malaysia, Thailand, and Indonesia has many potential medicinal uses. It is used as a traditional herbal medicine for treating many diseases including skin rashes. Skin rash often appear in an allergic reaction. Recently, we have shown that C. nutans aqueous extract has the ability to alleviate ovalbumin-induced active systemic anaphylaxis rats from anaphylaxis – the acute form of allergy. This present study is aimed at comparing the ability of ethanolic and aqueous extracts of C. nutans to suppress IgE-mast cell degranulation. Materials and Methods: IgE-presensitized rat basophilic leukemic (RBL-2H3) cells pretreated with C. nutans ethanolic (100% ethanolic, 70% and 50% aqueous ethanolic) or 100% aqueous extracts were challenged with dinitrophenyl-bovine serum albumin to analyze the release of early and late-phase pro-inflammatory mediators. Results: We found that at concentrations of 5 mg/ml and above, C. nutans aqueous extract was able to suppress the levels of β-hexosaminidase and histamine while suppression was not seen in ethanolic extracts pretreated RBL-2H3 cells.Conclusion: Hence, we proposed that C. nutans aqueous extract is more active compared to the ethanolic extracts in suppressing the mediators of IgE-mast cell degranulation.


How to cite this article:
Foong Kow AS, Fong LY, Khoo LW, Tan JW, Lee MT, Israf DA, Abas F, Shaari K, Tham CL. Clinacanthus nutans aqueous extract suppresses the release of histamine and β-Hexosaminidase inin-vitro model of IgE-mediated mast cell degranulation.Phcog Mag 2020;16:400-408


How to cite this URL:
Foong Kow AS, Fong LY, Khoo LW, Tan JW, Lee MT, Israf DA, Abas F, Shaari K, Tham CL. Clinacanthus nutans aqueous extract suppresses the release of histamine and β-Hexosaminidase inin-vitro model of IgE-mediated mast cell degranulation. Phcog Mag [serial online] 2020 [cited 2022 May 28 ];16:400-408
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2020;volume=16;issue=69;spage=400;epage=408;aulast=Foong;type=0