Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 15  |  Issue : 64  |  Page : 189--196

Cell cycle arrest and apoptosis in human liver cancer cell line and A549 cell lines by Turbiconol– A novel sterol isolated from Turbinaria conoides


Kala K Jacob1, KJ Prashob Peter2, S Muraleedharan Nair2, N Chandramohanakumar1 
1 Department of Chemical Oceanography, School of Marine Sciences, Cochin University of Science and Technology; Inter University Center for Development of Marine Biotechnology, Cochin University of Science and Technology, Cochin, Kerala, India
2 Department of Chemical Oceanography, School of Marine Sciences, Cochin University of Science and Technology, Cochin, Kerala, India

Correspondence Address:
Kala K Jacob
Inter University Center for Development of Marine Biotechnology, Cochin University of Science and Technology, Cochin - 682 016, Kerala
India

Background: Turbinaria conoides, a brown seaweed, is a rich source of oxygenated fucosterols which are capable of suppressing the proliferation of cancer cells. Their specific therapeutically significant biological activity is directly related to the unique structural features of the molecule. This study specifically focuses on extracting unconventional sterol molecules (side chain extension) from this seaweed which can be used as a lead molecule to evolve therapeutical agents. Materials and Methods: To isolate unconventional sterol molecule, for structural elucidation and bioactivity study, sufficient amount of T. conoides was collected from Mandapam, an unique biodiverse environment along the Southeast coast of India. State-of-the-art methods available for the purification and characterization of molecule (High-resolution fast-atom bombardment mass spectrometry, ultraviolet-visible spectroscopy, attenuated total reflection–fourier transform infra-red, One-dimensional nuclear magnetic resonance, and two-dimensional nuclear magnetic resonance) were put in. In vitro bioassays (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide, double staining, and flowcytometry) were carried out against A549 and human liver cancer cell line (HepG2) malignant cells to assess the cytostatic potential. Data were statistically validated. Results: A unique unconventional sterol molecule (Turbiconol) with ethyl and methyl group at C-27 was isolated. This molecule induced apoptosis in A549 and HepG2. However, cell cycle assessment revealed G0/G1 cell cycle arrest in Hep G2 and G2/M checkpoint was responsible for the suppression of A549 cell line. Conclusion: A novel unconventional compound, turbiconol, is reported in this study. In vitro results highlight the potential of this molecule in developing therapeutical combination which can be used for novel treatment methods.


How to cite this article:
Jacob KK, Prashob Peter K J, Nair S M, Chandramohanakumar N. Cell cycle arrest and apoptosis in human liver cancer cell line and A549 cell lines by Turbiconol– A novel sterol isolated from Turbinaria conoides.Phcog Mag 2019;15:189-196


How to cite this URL:
Jacob KK, Prashob Peter K J, Nair S M, Chandramohanakumar N. Cell cycle arrest and apoptosis in human liver cancer cell line and A549 cell lines by Turbiconol– A novel sterol isolated from Turbinaria conoides. Phcog Mag [serial online] 2019 [cited 2022 May 23 ];15:189-196
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2019;volume=15;issue=64;spage=189;epage=196;aulast=Jacob;type=0