Year : 2018 | Volume
: 14 | Issue : 59 | Page : 665--672
Comparative evaluation of antioxidant activity and liquid chromatography–Mass spectrometry-based phytochemical profiling of various biological parts of Caryota urens
Balaji Sujitha, Kavasseri Ganesan Kripa
Department of Biochemistry, Vels Institute of Science, Technology and Advanced Studies, Chennai, Tamil Nadu, India
Introduction: Caryota urens belongs to the palm family and is widely distributed in Asia. Conventionally, it is used to treat gastric ulcers, snake bites, migraine, and rheumatic swellings. The objectives of this study were to determine the phytochemical content and antioxidant activities of hydroalcoholic and aqueous (Aq) extracts of various parts of C. urens. Materials and Methods: Extractions of various parts of C. urens were performed by cold maceration using different solvents such as 70% ethanol and distilled water. The extracts were subjected to assessment of their antioxidant potential using various in vitro systems such as 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical, lipid peroxidation, and phosphomolybdenum reduction. The extract was subjected to Fourier-transform infrared spectroscopy, liquid chromatography–mass spectrometry (LC-MS), and high-performance liquid chromatography (HPLC) analysis to detect the phytoconstituents. Results: The total flavonoid and total phenol content was found to be highest in leaf hydroalcoholic extract (C. urens leaf hydroalcoholic extract [CULHA]), 43.84 ± 3.59 mg/g quercetin equivalent, and 41.68 ± 3.30 mg/g gallic acid equivalent, respectively. Hydroalcoholic extracts of C. urens (CULHA, C. urens fruit hydroalcoholic extract and C. urens bark hydroalcoholic extract) had the highest antioxidant activity when compared to Aq extracts. Phenolic acids, coumarins, carboxylic acids, and flavonoids were characterized by LC-MS. HPLC analysis further confirmed the presence of rutin, umbelliferone, and ferulic acid. Conclusion: A direct association between the high content of flavonoid rutin and antioxidant activity in leaf hydroalcoholic extract was noted. The fact that C. urens is rich in coumarins and rutin seems to explain the high antioxidant potential of this plant extract.
Abbreviations Used: DPPH: 1,1-diphenyl-2-picrylhydrazyl; LPO: Lipid peroxidation; SO: Superoxide; NBT: Nitroblue tetrazolium; PMS: Phenazine methosulfate; CULHA: C. urens leaf hydroalcoholic extract; CULAq: C. urens leaf aqueous extract; CUFHA: C. urens fruit hydroalcoholic extract; CUFAq: C. urens fruit aqueous extract; CUBHA: C. urens bark hydroalcoholic extract; CUBAq: C. urens bark aqueous extract; ROS: Reactive oxygen species; %: Percent; °C: Celsius; μg: Microgram; μl: Microliter; mg: Milligram; ml: Milliliter; OD: Optical density; IC50: Concentration yielding 50% inhibition; FTIR: Fourier-transform infrared spectroscopy; LC-MS: Liquid chromatography–mass spectrometry; GAE: Gallic acid equivalent; QE: Quercetin equivalent; AE: Ascorbic acid equivalent; TPC: Total phenolic content; TFC: Total flavonoid content; TAC: Total antioxidant activity.
Kavasseri Ganesan Kripa
Department of Biochemistry, Vels Institute of Science, Technology and Advanced Studies, Chennai - 600 117, Tamil Nadu
|How to cite this article:|
Sujitha B, Kripa KG. Comparative evaluation of antioxidant activity and liquid chromatography–Mass spectrometry-based phytochemical profiling of various biological parts of Caryota urens.Phcog Mag 2018;14:665-672
|How to cite this URL:|
Sujitha B, Kripa KG. Comparative evaluation of antioxidant activity and liquid chromatography–Mass spectrometry-based phytochemical profiling of various biological parts of Caryota urens. Phcog Mag [serial online] 2018 [cited 2022 Aug 9 ];14:665-672
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2018;volume=14;issue=59;spage=665;epage=672;aulast=Sujitha;type=0