Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 13  |  Issue : 52  |  Page : 886--889

Methyl jasmonate and salicylic acid enhanced the production of ursolic and oleanolic acid in callus cultures of Lepechinia Caulescens


Víctor M Vergara Martínez1, Samuel E Estrada-Soto2, José de Jesús Arellano-García1, Julio C Rivera-Leyva2, Patricia Castillo-España1, Angélica Flores Flores2, Alexandre T Cardoso-Taketa1, Irene Perea-Arango1 
1 Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México
2 Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México

Correspondence Address:
Irene Perea-Arango
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos
México

Background: The production of triterpenes from plants for pharmacological purposes varies in concentration, due to genetic and environmental factors. In vitro culture enables the control and increase of these bioactive molecules. Objective: To evaluate the effect of plant growth regulators and elicitors in the induction of calli and the production of ursolic acid (UA) and oleanolic acid (OA) in Lepechinia caulescens. Materials and Methods: Leaf explants were exposed for the induction of calli at different concentrations and combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). Methyl jasmonate (MJ) and salicylic acid were used as elicitors. High-performance liquid chromatography method was used to quantify UA and OA content in each treatment. Results: Treatment with 3.0 mg/L of 2,4-D and 0.1 mg/L of BAP produced the best results for calli induction and production of UA (1.57 mg/g dry weight [DW]) and OA (1.13 mg/g DW). Both elicitors facilitated the accumulation of triterpenes. Conclusion: The combination of auxins and cytokinins showed favorable results for the induction of calli. Variation concerning the accumulation of UA and OA was observed between treatments. MJ increased the production of triterpenes five times after 8 h of exposure, compared to control treatment. There is a greater accumulation of UA (16.58 mg/g DW) and OA (1.94 mg/g DW) in leaves of wild plants. Abbreviations used: 2,4-D: 2,4-dichlorophenoxyacetic acid, BAP: 6-benzylaminopurine, DW: Dry weight, MJ: Methyl jasmonate, OA: Oleanolic acid, PGRs: Plant growth regulators, UA: Ursolic acid, SA: Salicylic acid.


How to cite this article:
Vergara Martínez VM, Estrada-Soto SE, Arellano-García Jd, Rivera-Leyva JC, Castillo-España P, Flores AF, Cardoso-Taketa AT, Perea-Arango I. Methyl jasmonate and salicylic acid enhanced the production of ursolic and oleanolic acid in callus cultures of Lepechinia Caulescens.Phcog Mag 2017;13:886-889


How to cite this URL:
Vergara Martínez VM, Estrada-Soto SE, Arellano-García Jd, Rivera-Leyva JC, Castillo-España P, Flores AF, Cardoso-Taketa AT, Perea-Arango I. Methyl jasmonate and salicylic acid enhanced the production of ursolic and oleanolic acid in callus cultures of Lepechinia Caulescens. Phcog Mag [serial online] 2017 [cited 2022 Sep 29 ];13:886-889
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=52;spage=886;epage=889;aulast=Vergara;type=0