Year : 2017 | Volume
: 13 | Issue : 52 | Page : 822--827
Expression of hepatic cytochrome P450s in rats administered with Guibi-tang, a traditional herbal formula
Seong Eun Jin1, Hyekyung Ha1, Chang-Seob Seo1, Hyeun-Kyoo Shin1, Soo-Jin Jeong2
1 K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea
2 KM Convergence Research Division, Korea Institute of Oriental Medicine; Korean Medicine Life Science, University of Science and Technology, Daejeon, Republic of Korea
Objective: The aim of this study was to investigate the possible herb-drug interactions between the traditional herbal formula Guibi-tang (GBT; Guipi-tang, Kihi-to) and conventional drugs. Materials and Methods: GBT was orally administered to either male or female Sprague Dawley (SD) rats once daily at doses of 1000, 2000, or 5000 mg/kg/day for 13 weeks. The messenger ribonucleic acid (mRNA) expression of drug-metabolizing enzyme cytochrome P450 isozymes (cytochrome P450s; CYP1A1, 1A2, 2B1/2, 2C11, 2E1, 3A1, 3A2, and 4A1) was analyzed in hepatic tissues by reverse transcription-polymerase chain reaction. Results: Repeated oral administration of GBT did not significantly influence the mRNA expression of hepatic CYP1A1, 1A2, 2B1/2, 2C11, 2E1, 3A1, 3A2, and 4A1 in male rats. By contrast, in female rats, the mRNA expression of hepatic CYP1A2 and 2B1/2 was significantly increased by repeated GBT treatment. Conclusion: Our findings indicate that caution is required in females when GBT is taken concomitantly with conventional drugs metabolized by CYP1A2 or 2B1/2. Our results provide information regarding the safety and effectiveness of GBT for clinical use.
Abbreviations used: CYP450: Cytochrome P450s, GBT: Guibi-tang, SD: Sprague Dawley, HPLC: High-performance liquid chromatography, OECD: Organization for Economic Cooperation and Development, RNA: Ribonucleic acid, RT-PCR: Reverse transcription-polymerase chain reaction, GADPH: Glyceraldehyde-3-phosphate dehydrogenase.
KM Convergence Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-Daero, Yuseong-Gu, Daejeon 34054
Republic of Korea
|How to cite this article:|
Jin SE, Ha H, Seo CS, Shin HK, Jeong SJ. Expression of hepatic cytochrome P450s in rats administered with Guibi-tang, a traditional herbal formula.Phcog Mag 2017;13:822-827
|How to cite this URL:|
Jin SE, Ha H, Seo CS, Shin HK, Jeong SJ. Expression of hepatic cytochrome P450s in rats administered with Guibi-tang, a traditional herbal formula. Phcog Mag [serial online] 2017 [cited 2022 Sep 25 ];13:822-827
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=52;spage=822;epage=827;aulast=Jin;type=0