Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 13  |  Issue : 52  |  Page : 607--612

Helichrysetin induces DNA damage that triggers JNK-mediated apoptosis in Ca Ski cells


Ho Yen Fong, Sri Nurestri Abd Malek, Hui Shin Yee, Saiful Anuar Karsani 
 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

Correspondence Address:
Saiful Anuar Karsani
Faculty of Science, Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur
Malaysia

Background: Cervical cancer has become one of the most common cancers in women and currently available treatment options for cervical cancer are very limited. Naturally occurring chalcones and its derivatives have been studied extensively as a potential anticancer agent in different types of cancer and helichrysetin is naturally occurring chalcone that possess potent antiproliferative activity toward human cancer cells. Materials and Methods: Inhibitory activity of helichrysetin was evaluated at different concentrations. Ability of helichrysetin to induce apoptosis and its relation with c-Jun N-terminal kinase (JNK)-mediated mechanism of apoptosis was assessed using flow cytometry and Western blotting. Results: Helichrysetin inhibited Ca Ski cells at half maximal inhibitory concentration 30.62 ± 0.38 μM. This compound has the ability to induce DNA damage, mitochondrial membrane disruption, and loss of cell membrane integrity. We have shown that apoptosis was induced through the activation of JNK-mediated apoptosis by DNA damage in the cells then triggering p53-downstream apoptotic pathway with increased expression of pro-apoptotic proteins, Bax and caspase 3, and suppression of Bcl-2 anti-apoptotic protein. DNA damage in the cells also caused phosphorylation of protein ataxia-telangiectasia mutated, an activator of DNA damage response. Conclusion: We conclude that helichrysetin can inhibit Ca Ski cells through DNA damage-induced JNK-mediated apoptotic pathway highlighting the potential of this compound as anticancer agent for cervical cancer. Abbreviations used: ATM: Ataxia-telangiectasia mutated, DAPI: 4',6-diamidino-2-phenylindole, DMSO: Dimethyl sulfoxide, FITC: Fluorescein isothiocyanate, IC50: Half maximal inhibitory concentration, JC1-5,5',6,6'-Tetrachloro: 1',3,3'-tetraethylbenzimidazolylcarbocyanine, iodide, JNK: c-Jun N-terminal kinase, MMP: Mitochondrial membrane potential, PBS: Phosphate-buffered saline, SRB: Sulforhodamine B, TUNEL: Terminal deoxynucleotidyl transferase dUTP nick labeling


How to cite this article:
Fong HY, Abd Malek SN, Yee HS, Karsani SA. Helichrysetin induces DNA damage that triggers JNK-mediated apoptosis in Ca Ski cells.Phcog Mag 2017;13:607-612


How to cite this URL:
Fong HY, Abd Malek SN, Yee HS, Karsani SA. Helichrysetin induces DNA damage that triggers JNK-mediated apoptosis in Ca Ski cells. Phcog Mag [serial online] 2017 [cited 2022 Sep 25 ];13:607-612
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=52;spage=607;epage=612;aulast=Fong;type=0