Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 13  |  Issue : 49  |  Page : 21--24

Nardostachys jatamansi root extract modulates the growth of IMR-32 and SK-N-MC neuroblastoma cell lines through MYCN mediated regulation of MDM2 and p53


Snehal Suryavanshi, Prerna Raina, Rashmi Deshpande, Ruchika Kaul-Ghanekar 
 Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth University, Katraj-Dhankawadi, Pune, Maharashtra, India

Correspondence Address:
Dr. Ruchika Kaul-Ghanekar
Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth Deemed University, IRSHA, Pune-Satara Road, Katraj-Dhankawadi, Maharashtra
India

Aim: The present study evaluated the effect of ethanolic extract of Nardostachys jatamansi roots (NJet) on MYCN mediated regulation of expression of MDM2 and p53 proteins in neuroblastoma cell lines, IMR-32 and SK-N-MC. Materials and Methods: The effect of NJet on cell viability was determined by MTT; and on growth kinetics was evaluated by trypan blue dye exclusion method and soft agar assay. The expression of p53, MDM2 and MYCN proteins in response to NJet treatment was evaluated by immunoblotting. Results: NJet decreased the viability of neuroblastoma cells without affecting the viability of non-cancerous, HEK-293 cells. It altered the growth kinetics of the cancer cells in a dose-dependent manner. NJet down regulated the expression of MYCN and MDM2 proteins with a simultaneous increase in the expression of tumor suppressor protein p53. Conclusions: The present data demonstrated that NJet regulated the growth of IMR-32 and SK-N-MC through reduction in MYCN expression that lead to down regulation of MDM2 protein and increase in p53 expression. These preliminary results warrant further in depth studies to explore the therapeutic potential of Nardostachys jatamansi in the management of neuroblastoma. Abbreviations used: NJet: N. jatamansie thanolic extract; MTT: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenylthiazolium bromide; FBS: Fetal bovine serum; FITC: Fluorescein isothiocyanate


How to cite this article:
Suryavanshi S, Raina P, Deshpande R, Kaul-Ghanekar R. Nardostachys jatamansi root extract modulates the growth of IMR-32 and SK-N-MC neuroblastoma cell lines through MYCN mediated regulation of MDM2 and p53.Phcog Mag 2017;13:21-24


How to cite this URL:
Suryavanshi S, Raina P, Deshpande R, Kaul-Ghanekar R. Nardostachys jatamansi root extract modulates the growth of IMR-32 and SK-N-MC neuroblastoma cell lines through MYCN mediated regulation of MDM2 and p53. Phcog Mag [serial online] 2017 [cited 2022 Aug 9 ];13:21-24
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=49;spage=21;epage=24;aulast=Suryavanshi;type=0