Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 13  |  Issue : 49  |  Page : 153--158

Trichosanthes kirilowii exerts androgenic activity via regulation of PSA and KLK2 in 22Rv1 prostate cancer cells


Soo-Jin Jeong1, Ji-Yoon Choi2, Mi-Sook Dong2, Chang-Seob Seo3, Hyeun-Kyoo Shin3 
1 KM Convergence Research Division, Korea Institute of Oriental Medicine, Deajeon; Korean Medicine Life Science, University of Science and Technology, Daejeon, Republic of Korea
2 School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea
3 K-herb Research Center, Korea Institute of Oriental Medicine, Deajeon, Republic of Korea

Correspondence Address:
Hyeun-Kyoo Shin
K-herb Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon
Republic of Korea

Background: The androgen comprises a group of hormones that play roles in male reproductive activity as well as personal characteristics. Objective: We investigated the androgenic activity of various herbal medicines in human prostate cancer 22Rv1 cells. Materials and Methods: Herbal extracts of Trichosanthes kirilowii (TK), Asarum sieboldii (AS), Sanguisorba officinalis (SO), and Xanthium strumarium (XS) were selected to have androgenic effects based on a preliminary in vitro screening system. Results: TK, AS, SO, and XS enhanced the proliferation of 22Rv1 cells without having cytotoxic effects. All tested herbal extracts increased androgen receptor (AR)-induced transcriptional activity in the absence or presence of dihydrotestosterone (DHT). In an AR-binding assay, TK, but not AS, SO, or XS, produced a significant inhibition of AR binding activity, indicating it has androgenic activity. Additionally, TK treatment positively regulated mRNA expression of the AR-related molecular targets prostate-specific antigen (PSA) and kallikrein 2 (KLK2) compared with untreated control. Conclusion: Taken together, TK-enhanced AR-mediated transcriptional activity might be an attractive candidate drug for treating androgen-related diseases. Abbreviations used: BPH: benign prostatic hyperplasia; AR: androgen receptor; DHT: dihydrotestosterone; PSA: prostate-specific antigen; TK: Trichosanthes kirilowii; AS: Asarum sieboldii; SO: Sanguisorba officinalis; XS: Xanthium strumarium; ATCC: American Type Culture Collection; FBS: fetal bovine serum; PBS: phosphate-buffered saline; SD: standard deviation; ARE: androgen-responsive element; KLK: kallikrein


How to cite this article:
Jeong SJ, Choi JY, Dong MS, Seo CS, Shin HK. Trichosanthes kirilowii exerts androgenic activity via regulation of PSA and KLK2 in 22Rv1 prostate cancer cells.Phcog Mag 2017;13:153-158


How to cite this URL:
Jeong SJ, Choi JY, Dong MS, Seo CS, Shin HK. Trichosanthes kirilowii exerts androgenic activity via regulation of PSA and KLK2 in 22Rv1 prostate cancer cells. Phcog Mag [serial online] 2017 [cited 2022 Aug 12 ];13:153-158
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=49;spage=153;epage=158;aulast=Jeong;type=0