Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 13  |  Issue : 49  |  Page : 136--140

High performance liquid chromatography-diode array detector method for the simultaneous determination of five compounds in the pulp and seed of sea buckthorn


Lu Zhao1, E Wen2, Halmuart Upur3, Shuge Tian3 
1 College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi, Xinjiang, China
2 College of TCM, Xinjiang Medical University, Urumqi, Xinjiang, China
3 Central Laboratory of Xinjiang Medical University, Urumqi, Xinjiang, China

Correspondence Address:
Prof. Shuge Tian
Central Laboratory of Xinjiang Medical University, Urumqi, Xinjiang
China

Context: Sea buckthorn (Hippophae rhamnoides L.) as a traditional Chinese medicinal plant has various uses in Xinjiang. Objective: A reversed-phase rapid-resolution liquid-chromatography method with diode array detector was developed for simultaneous determination of protocatechuic acid, rutin, quercetin, kaempferol, and isorhamnetin in the pulp and seed of sea buckthorn, a widely used traditional Chinese medicine for promoting metabolism and treating scurvy and other diseases. Settings and design: Compounds were separated on an Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm; USA) with gradient elution using methanol and 0.4% phosphoric acid (v/v) at 1.0 mL/min. Detection wavelength was set at 280 nm. Materials and Methods: The fruits of wild sea buckthorn were collected from Wushi County in Aksu, Xinjiang Province. Statistical performances: The RSD of precision test of the five compounds were in the range of 0.60-2.22%, and the average recoveries ranged from 97.36% to 101.19%. Good linearity between specific chromatographic peak and component qualities were observed in the investigated ranges for all the analytes (R2 > 0.9997). Results: The proposed method was successfully applied to determine the levels of five active components in sea buckthorn samples from Aksu in Xinjiang. Conclusions: The proposed method is simple, fast, sensitive, accurate, and suitable for quantitative assessment of the pulp and seed of sea buckthorn. Abbreviation used: PR: protocatechuic acid, RU: rutin, QU: quercetin, KA: kaempferol, IS: isorhamnetin, HPLC: high-performance liquid chromatography, HPLC-DAD: high performance liquid chromatographydiode array detector, LOD: linearity and limit of detection, LOQ: limit of quantitation, RSD: relative standard deviation


How to cite this article:
Zhao L, Wen E, Upur H, Tian S. High performance liquid chromatography-diode array detector method for the simultaneous determination of five compounds in the pulp and seed of sea buckthorn.Phcog Mag 2017;13:136-140


How to cite this URL:
Zhao L, Wen E, Upur H, Tian S. High performance liquid chromatography-diode array detector method for the simultaneous determination of five compounds in the pulp and seed of sea buckthorn. Phcog Mag [serial online] 2017 [cited 2022 Aug 10 ];13:136-140
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=49;spage=136;epage=140;aulast=Zhao;type=0