Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 13  |  Issue : 49  |  Page : 108--113

Antioxidant and hepatoprotective potential of phenol-rich fraction of Juniperus communis Linn. leaves


Akash Ved1, Amresh Gupta1, Ajay Kumar Singh Rawat2 
1 Goel Institute of Pharmacy and Sciences, Lucknow, Uttar Pradesh, India
2 National Botanical Research Institute, Lucknow, Uttar Pradesh, India

Correspondence Address:
Prof. Akash Ved
Goel Institute of Pharmacy and Sciences, Faizabad Road, Lucknow, Uttar Pradesh
India

Background: Juniperus communis Linn. is an important plant in India traditional system of medicine which is widely used by different tribes in many countries. Objective: In the present study, the antioxidant, cytotoxic and hepatoprotective activities of Juniperus communis leaves were investigated against various models. Materials and Methods: ethanolic extract (70% v/v) of J. communis leaves was successively extracted using hexane and ethyl acetate to prepare various fractions. Total phenol content was resolute by the Folin-Ciocalteau's process. The antioxidant properties of the different fractions/extract of leaves of J. communis were examined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and Fe2+ chelating ability. Cytotoxic activity was examined by cell viability assay on HepG2 cells. Hepatoprotective activity of ethyl acetate fraction (EAF) evaluated against PCM-Paracetamol-induced hepatic damage in Wistar albino rats. Results: Total phenol content was found maximum 315.33 mg/GAE/g in EAF. Significant scavenging activity were found for EAF (IC50 = 177 μg/ml) as compared to standard BHT (IC50 = 138 μg/ml), while EAF showed good Fe2+ chelating ability having an IC50 value of 261 mg/ML compared to standard ethylenediaminetetraacetic acid (7.7 mg/mL). It was found that EAF treated group shows remarkable decrease in serum Aspartate aminotransferase, serum Alanine aminotransferase, total bilirubin, direct bilirubin, and alkaline phosphatase level in treatment group as compared to the hepatotoxic group. Conclusion: EAF of J. communis leaves is found to be potent antioxidant and hepatoprotective without any cytotoxicity and it can also be included in nutraceuticals with notable benefits for mankind or animal health. Abbreviations used: HepG2: Liver hepatocellular carcinoma, BHT: Butylated hydroxytoluene, PCM: Paracetamol, IC50: Half maximal inhibitory concentration, RSA: Radical Scavenging Activity, WST: Water-soluble tetrazolium.


How to cite this article:
Ved A, Gupta A, Rawat AK. Antioxidant and hepatoprotective potential of phenol-rich fraction of Juniperus communis Linn. leaves.Phcog Mag 2017;13:108-113


How to cite this URL:
Ved A, Gupta A, Rawat AK. Antioxidant and hepatoprotective potential of phenol-rich fraction of Juniperus communis Linn. leaves. Phcog Mag [serial online] 2017 [cited 2022 Aug 15 ];13:108-113
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=49;spage=108;epage=113;aulast=Ved;type=0