Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 12  |  Issue : 47  |  Page : 471--474

Assessment of In vitro antibacterial activity and cytotoxicity effect of Nigella sativa oil


Ayse Ruveyda Ugur1, Hatice Turk Dagi2, Bahadir Ozturk3, Gulsum Tekin3, Duygu Findik2 
1 Department of Medical Microbiology, Konya Education and Research Hospital, Konya, Turkey
2 Department of Medical Microbiology, Faculty of Medicine, Selcuk University, Konya, Turkey
3 Department of Biochemistry, Faculty of Medicine, Selcuk University, Konya, Turkey

Correspondence Address:
Ayse Ruveyda Ugur
Department of Medical Microbiology, Konya Education and Research Hospital, Meram Yeniyol Caddesi No. 97, 42090 Meram, Konya
Turkey

Background: Methicillin resistance is a serious health concern since it has spread among Staphylococcus aureus and coagulase-negative Staphylococci (CoNS) that are frequent community and nosocomial pathogens worldwide. Methicillin-resistant strains are often resistant to other classes of antibiotics, making their treatment difficult. Nigella sativa oil is known to be active against Gram-positive cocci, yet its in vitro cytotoxicity is rarely investigated, is a proper and powerful candidate for treatment of methicillin-resistant isolates. Objectives: The aim of this study is to evaluate the in vitro antibacterial activity and cytotoxicity effect of N. sativa oil. Materials and Methods: The minimal inhibitory concentrations (MICs) of N. sativa oil were determined by broth microdilution method against four different American Type Culture Collection strains, 45 clinical isolates of methicillin-resistant S. aureus (MRSA), and 77 methicillin-resistant CoNS (MRCoNS). The effects of different dilutions (0.25 μg/mL, 0.5 μg/mL, and 1 μg/mL) of N. sativa oil on the proliferation of gingival fibroblasts were evaluated. Results: The MIC values of N. sativa oil against clinical isolates of Staphylococci were between <0.25 μg/mL and 1.0 μg/mL. Compared to the control group, there was no cytotoxic effect on the proliferation of the gingival fibroblasts. Conclusion: In the present study, the oil of N. sativa was very active against MRSA and MRCoNS and had no in vitro cytotoxicity at relevant concentrations. These findings emphasize that there is a requirement for further clinical trials on N. sativa oil for «DQ»safe«DQ» medical management of infections caused by methicillin-resistant Staphylococci. Abbreviation used: ATCC: American Type Culture Collection; CLSI: Clinical and Laboratory Standards Institute; CoNS: Coagulase-negative Staphylococci; DMEM: Dulbecco«SQ»s modified Eagle«SQ»s medium; DMSO: Dimethyl sulfoxide; FBS: Fetal bovine serum; HGF: Human gingival fi broblast; MIC: Minimal inhibitory concentration; MRCoNS: Methicillin-resistant CoNS;MRSA: Methicillin-resistant S. aureus


How to cite this article:
Ugur AR, Dagi HT, Ozturk B, Tekin G, Findik D. Assessment of In vitro antibacterial activity and cytotoxicity effect of Nigella sativa oil.Phcog Mag 2016;12:471-474


How to cite this URL:
Ugur AR, Dagi HT, Ozturk B, Tekin G, Findik D. Assessment of In vitro antibacterial activity and cytotoxicity effect of Nigella sativa oil. Phcog Mag [serial online] 2016 [cited 2022 May 23 ];12:471-474
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2016;volume=12;issue=47;spage=471;epage=474;aulast=Ugur;type=0