Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 11  |  Issue : 41  |  Page : 226--231

Technical aspects on production of fluid extract from Brosimum gaudichaudii Trécul roots


Frederico Severino Martins, Henrique Pascoa, José Realino de Paula, Edemilson Cardoso da Conceição 
 Department of Pharmacy, Faculty of Pharmacy Research, Federal University of Goiás, Goiás, Brazil

Correspondence Address:
Frederico Severino Martins
Department of Pharmacy, Faculty of Pharmacy Research, Federal University of Goiás, Goiás
Brazil

Instruction: Despite the increased use of Brosimum gaudichaudii roots as raw material on medicine to treatment of vitiligo, there are not studies that showing the impact of unit operations on the quality and standardized of the extract of B. gaudichaudii. The quality of the herbal extract is essential to ensure the safety and efficacy of pharmaceutical product. Due the medical and commercial importance, this study aimed to evaluate the impact of the extraction method (ultrasound or percolation) on the quality of herbal extract and optimize the extraction of psoralen and 8-methoxypsoralen (8-MOP) from B. gaudichaudii. Materials and Methods: The extraction recovery was evaluate by high-performance liquid chromatography (C8 reverse phase column and acetonitrile: Water 45:55 and flow rate 0.6 mL/min). The extraction was performed by ultrasound-assisted extraction (UEA) or percolation using a Box-Behnken design. Results: From both chemical markers (psoralen and bergapten), the optimal conditions for the UEA were an extraction time of 25 min, the mean particle size of 100 μm, and an ethanol: Water ratio of 55:45 (v/v). Conclusion: The extraction by percolation revealed that ethanol 55% was more efficient than ethanol 80% to extract psoralen and bergapten.


How to cite this article:
Martins FS, Pascoa H, de Paula JR, da Conceição EC. Technical aspects on production of fluid extract from Brosimum gaudichaudii Trécul roots.Phcog Mag 2015;11:226-231


How to cite this URL:
Martins FS, Pascoa H, de Paula JR, da Conceição EC. Technical aspects on production of fluid extract from Brosimum gaudichaudii Trécul roots. Phcog Mag [serial online] 2015 [cited 2022 May 28 ];11:226-231
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2015;volume=11;issue=41;spage=226;epage=231;aulast=Martins;type=0