Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 10  |  Issue : 38  |  Page : 430--433

Cytotoxicity of gypsogenic acid isolated from Gypsophila trichotoma


Ilina Krasteva1, Maya Yotova1, Deyan Yosifov2, Niko Benbassat1, Kristina Jenett-Siems3, Spiro Konstantinov2 
1 Department of Pharmacognosy, Faculty of Pharmacy, Medical University, Sofia, Bulgaria
2 Laboratory for Experimental Chemotherapy, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia, Bulgaria
3 2Institute of Pharmacy, Free University Berlin, Berlin, Germany

Correspondence Address:
Ilina Krasteva
Department of Pharmacognosy, Faculty of Pharmacy, 2 Dunav St., 1000, Sofia, Bulgaria

Background: Gypsophila trichotoma Wend. (Caryophyllaceae) is a medicinal plant which is protected in Bulgaria by the Biodiversity Law. Previous studies have showed the presence of triterpene saponins, sterols, flavonoids, triterpens, etc. Objective: Gypsogenic acid, isolated from Gypsophila trichotoma roots, was evaluated for cytotoxic activity. Materials and Methods: The structure of the compound was elucidated by spectral methods. The cell survival fraction was determined by the MTT dye reduction assay, performed with some modifications. Results: Gypsogenic acid was tested in a panel of human tumor cell lines and was found to inhibit the proliferation of malignant cells. It was active against leukemic cells with lymphoid (SKW-3 and BV-173) or myeloid phenotype (HL-60, K-562, and LAMA-84), as well as against the EJ bladder carcinoma cell line. Bcr-Abl expressing myeloid cells (LAMA-84 and especially K-562) displayed lower sensitivity. HL-60/Dox cells were less sensitive to gypsogenic acid than the parent cell line, which shows that gypsogenic acid is probably a substrate of MRP-1.


How to cite this article:
Krasteva I, Yotova M, Yosifov D, Benbassat N, Jenett-Siems K, Konstantinov S. Cytotoxicity of gypsogenic acid isolated from Gypsophila trichotoma.Phcog Mag 2014;10:430-433


How to cite this URL:
Krasteva I, Yotova M, Yosifov D, Benbassat N, Jenett-Siems K, Konstantinov S. Cytotoxicity of gypsogenic acid isolated from Gypsophila trichotoma. Phcog Mag [serial online] 2014 [cited 2022 May 22 ];10:430-433
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2014;volume=10;issue=38;spage=430;epage=433;aulast=Krasteva;type=0