Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2013  |  Volume : 9  |  Issue : 33  |  Page : 23--27

Anti-glycated and antiradical activities in vitro of polysaccharides from Ganoderma capense


Chunyan Yan, Fansheng Kong, Dezhi Zhang, Jiangxia Cui 
 Department of Natural Medicinal Chemistry, College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China

Correspondence Address:
Chunyan Yan
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006
China

Background : Ganoderma capense is a Ganoderma species and is widely used, especially in Asia, as a well-known medicinal mushroom for health-promoting effect and for treatment of chronic diseases, such as diabetes, aging, etc. G. capense is rich of polysaccharide. Objective: To isolate the polysaccharides from G. capense and evaluate their anti-glycated and antiradical activities in vitro. Materials and Methods : The dried powder of submerged fermentation culturing mycelium of G. capense was defatted, extracted with water/ alkaline water followed by ethanol precipitation and deproteinated. And four crude polysaccharides, named as GC50, GC70, GC90 and GCB, were obtained. For the first time, the in vitro anti-glycated activities of the four samples were studied by non-enzymatic glycation reaction. Then, the DPPH radical and hydroxyl radical assays were established to estimate the antiradical capacity of the four samples. Meanwhile the contents of polysaccharides were determined by phenol-sulphuric acid colorimetry. Results and Conclusion : Preliminary antiradical in vitro studies indicated that the four crude polysaccharides showed concentration-dependent scavenging abilities on DPPH and hydroxyl radicals. The evaluation of anti-glycation activity suggested that GC70 had good potential for inhibiting the formation of advanced glycation end products. Time- and dose-dependent effects were also observed for all GC70 samples.


How to cite this article:
Yan C, Kong F, Zhang D, Cui J. Anti-glycated and antiradical activities in vitro of polysaccharides from Ganoderma capense.Phcog Mag 2013;9:23-27


How to cite this URL:
Yan C, Kong F, Zhang D, Cui J. Anti-glycated and antiradical activities in vitro of polysaccharides from Ganoderma capense. Phcog Mag [serial online] 2013 [cited 2022 May 22 ];9:23-27
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2013;volume=9;issue=33;spage=23;epage=27;aulast=Yan;type=0