Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2010  |  Volume : 6  |  Issue : 22  |  Page : 111--115

Simultaneous determination of five marker constituents in Ssanghwa tang by HPLC/DAD


Jin Bae Won1, Jin Yeul Ma2, Young Ran Um2, Choong Je Ma3 
1 Department of Biomaterials Engineering, School of Biotechnology and Bioengineering, Kangwon National University, Chuncheon 200-701, Korea
2 TKM Converging Research Division, Korea Institute of Oriental Medicine, 483 Exporo, Yuseong-gu, Daejeon 305-811, Korea
3 Department of Biomaterials Engineering, School of Biotechnology and Bioengineering, Kangwon National University, Chuncheon 200-701; and Research Institute of Biotechnology, Kangwon National University, Chuncheon 200-701, Korea

Correspondence Address:
Choong Je Ma
Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University, Hyoja-2 Dong, Chuncheon 200-701
Korea

A HPLC-DAD method was established for the simultaneous evaluation of five bioactive compounds in Ssanghwa tang (SHT) including glycyrrhizin, paeoniflorin, cinnamic acid, decursin and 6-gingerol. These compounds were separated in less than 40 min using a Dionex C 18 column with a gradient elution system of water and methanol at a flow rate of 1 ml/min. Calibration curve of standard components presented excellent linear regression (R 2 > 0.9903) within the test range. Limit of detection and limit of quantification varied from 0.07 to 0.46 μg/ml and 0.13 to 1.11 μg/ml, respectively. The relative standard deviations (RSDs) of data of the intraday and interday experiments were less than 3.67 and 5.73%, respectively. The accuracy of recovery test ranged from 95.98 to 105.88% with RSD values 0.10- 4.82%.


How to cite this article:
Won JB, Ma JY, Um YR, Ma CJ. Simultaneous determination of five marker constituents in Ssanghwa tang by HPLC/DAD.Phcog Mag 2010;6:111-115


How to cite this URL:
Won JB, Ma JY, Um YR, Ma CJ. Simultaneous determination of five marker constituents in Ssanghwa tang by HPLC/DAD. Phcog Mag [serial online] 2010 [cited 2022 May 27 ];6:111-115
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2010;volume=6;issue=22;spage=111;epage=115;aulast=Won;type=0