Anti-nociceptive effect of 7-methoxy coumarin from Eupatorium Triplinerve vahl (Asteraceae)

Binoy Varghese Cheriyan; Parimala Kadhirvelu; Jagan Nadipelly; Jaikumar Shanmugasundaram; Vijaykumar Sayeli; Viswanathan Subramanian

Articles

Abstract

Pharmacognosy Magazine,2017,13,49,81-84.

DOI:10.4103/0973-1296.197650

Published:January 2017

Type:Original Article

Authors:

Author(s) affiliations:

Binoy Varghese Cheriyan¹, Parimala Kadhirvelu², Jagan Nadipelly³, Jaikumar Shanmugasundaram², Vijaykumar Sayeli⁴, Viswanathan Subramanian²
¹Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Vels University, Pallavaram, Chennai, India
²Department of Pharmacology, Meenakshi Medical College and Research Institute, Enathur, Kanchipuram, India
³Department of Pharmacology, MNR Medical College and Hospital, Fasalwadi, Sangareddy, Medak, Telangana, India
⁴Department of Pharmacology, Mamata Medical College and Hospital, Rotary Nagar, Khammam, Telangana, India

Abstract:

Aim: To evaluate the anti-nociceptive activity of 7-methoxy coumarin isolated from ethyl acetate fraction of the alcoholic extract of Eupatorium triplinerve Vahl. Materials and Methods: The shade dried leaves of E. triplinerve were extracted with ethyl alcohol and the extract was condensed. This extract was fractionated with n-hexane, ethyl acetate, and n-butanol. The ethyl acetate fraction was subjected to column chromatography which yielded a crystalline compound-A, which was investigated for spectral characteristics. Pharmacological studies: The isolated compound-A was subjected to behavioral studies and anti-nociceptive evaluation in mice by acetic acid induced writhing and formalin induced nociception. Results: The spectral studies indicated that the structure of compound-A complies with 7- methoxy coumarin. Pre-treatment with 7-methoxy coumarin reduced the number of abdominal constrictions in mice and decreased the time spent in paw licking and biting response in formalin assay. There were no significant behavioral changes. Conclusion: A dose dependent anti-nociceptive action of 7- methoxy coumarin was revealed by the present experiments which support the traditional use of E. triplinerve in pain and inflammatory disorders.