A study on the quality evaluation of agarwood of Aquilaria sinensis and Aquilaria malaccensis induced by different inducers based on gray correlation degree and TOPSIS

Zhiling Zhuang; Shenghong Wu; Shimin Deng; Pengjian Zhu; Xin Zhou; Xiaoying Chen; Weimin Zhang; Xiaoxia Gao

Articles

Abstract

Pharmacognosy Magazine,2022,18,79,713-728.

DOI:10.4103/pm.pm_340_21

Published:September 2022

Type:Original Article

Authors:

Author(s) affiliations:

Zhiling Zhuang¹, Shenghong Wu², Shimin Deng¹, Pengjian Zhu¹, Xin Zhou¹, Xiaoying Chen¹, Weimin Zhang³, Xiaoxia Gao⁴
¹School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China
²School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou; Research Institution of Biology, Zhuhai United Laboratories Co., Ltd., Zhuhai, China
³State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China
⁴School of Pharmacy, Guangdong Pharmaceutical University; State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China

Abstract:

Background: Agarwood is a resinous heartwood produced in certain Aquilaria species that is often used as a spice and Chinese medicine materials. Objectives: This study aimed to comprehensively evaluate the agarwood quality of different species under the same inducer as well as different inducers of the same species. Materials and Methods: The GC-MS data of agarwood were retrieved by AMDIS to obtain 47 secondary metabolites. Combined with multivariate statistical analysis, 12 secondary metabolites were identified as potentially representing the differences between A. malaccensis and A. sinensis. The gray correlation degree and TOPSIS method were used to comprehensively grade 18 characters, including the AEC, the content of agarotetrol, the apparent abundance of GC-MS fingerprint, and the 15 secondary metabolites representing the 58 batches of samples. The OD values representing the overall desirability of r_i (gray correlation degree) and c_i (TOPSIS) were ranked, with a higher ranking reflecting, better agarwood quality. Results: The ranking results demonstrated that the agarwood samples of the top 33 in OD value were all induced by FAM71, whereas the agarwood samples of the top 23 were all from A. malaccensis. The agarwood of A. sinensis induced by FA had the lowest OD value. Conclusion: The study demonstrated that the quality of agarwood from A. malaccensis was better than that of A. sinensis using the same inducer of FAM71. In the same species of A. sinensis, the quality of agarwood produced by FAM71 was better than that induced by formic acid alone or NA8 alone. This study provided a theoretical basis for the selection of high-quality agarwood inducer and tree species, as well as a reference basis for the efficient production of agarwood in the actual production process.