Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2022  |  Volume : 18  |  Issue : 79  |  Page : 692-698

In vivo antitumor and immune effects of Paris polyphylla rhizome and tinosporae radix extracts administered by different modes


1 Key Laboratory of Modern Preparation of TCM, Jiangxi University of Traditional Chinese Medicine, Ministry of Education, Nanchang 330004, China
2 Library of Jiangxi University of Traditional Chinese Medicine, Nanchang, China
3 College of Life Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang, China

Correspondence Address:
Yonghong-Liang
Key Laboratory of Modern Preparation of TCM, Jiangxi University of Traditional Chinese Medicine, Ministry of Education, Nanchang - 330004
China
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_6_22

Rights and Permissions

Objectives: To investigate the effects of gavage and transdermal administration on the anti-tumor activity of QJ623 (extracts of Paris polyphylla rhizome and Tinosporae Radix) on a murine H22 solid tumor transplantation model. Materials and Methods: H22 cell suspensions were diluted to a density of 4 × 105 cells/mL with sterile saline and then injected into 6-week-old mice subcutaneously (0.2 mL) into the right anterior axilla to create a murine model of liver cancer. Tumor tissues were collected after drug administration. The tumor growth-suppression rate was calculated, and the tumor tissues were stained with hematoxylin and eosin to detect necrosis. Terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling assay was performed to observe the apoptosis. The expression of p-ACK1, p-AKT, Bax, Bcl-2, and Caspase3 in the tumor tissues was detected by western blotting. Bio-chemical kits were used to detect the serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine (Cre), blood urea nitrogen (BUN), and uric acid (UA) for liver and kidney function analyses. Serum levels of IL-6, TNF-α, IFN-γ, and IL-10 were determined using enzyme-linked immuno-sorbent assay. Results: Gavage and transdermal administration of QJ623 at different concentrations reduced the tumor mass and volume to different degrees; promoted tumor cell apoptosis; decreased the expression levels of the p-AKT and Bcl-2 proteins; increased the expression of the apoptotic proteins Bax and Caspase3; increased the number of Th1 (IFN-γ) cells; decreased the number of Th2 (IL-4) and Treg cells; reduced the serum AST, ALT, BUN, UA, IL-6, TNF-α, and IFN-γ levels; and increased the IL-10 levels. Conclusion: Both gavage and transdermal administration of QJ623 showed anti-tumor effects by promoting tumor cell apoptosis, decreasing the level of inflammatory factors, increasing the number of Th1 (IFN-γ) immune cells, and decreasing the numbers of Th2 (IL-4) and Treg immune cells.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed82    
    Printed0    
    Emailed0    
    PDF Downloaded12    
    Comments [Add]    

Recommend this journal