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Year : 2022  |  Volume : 18  |  Issue : 78  |  Page : 400-405

Celastrus paniculatus seed oil ameliorates oxidative stress in lipopolysaccharide-induced respiratory inflammation in mice

1 Department of Microbiology and Cell Biology, Indian Institute of Science, Bengaluru, Karnataka, India
2 Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bengaluru, Karnataka, India

Correspondence Address:
William R Surin
Principal Research Scientist, Department of Microbiology and Cell Biology, Indian Institute of Science, Bengaluru - 560012, Karnataka
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/pm.pm_575_21

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Background: Celastrus paniculatus have been reported to possess various medicinal properties. However, very little is known about its effect on respiratory inflammation. Objectives: We evaluated Celastrus paniculatus seed oil (CP oil) on lipopolysaccharide (LPS)-induced mice model of respiratory inflammation. Materials and Methods: Both short-term and long-term studies were carried out. In short-term study, C57BL/6 mice were exposed to LPS (10 mg/kg) by intranasal route for one week. CP oil (1 g/kg) was given orally on the 1st, 3rd and 5th day. All the mice were sacrificed on the 7th day. In long-term study, C57BL/6 mice were exposed to LPS (10 mg/kg) by the intranasal route for one month. CP oil (1 g/kg) or theophylline (1 mg/kg) was administered twice a week for one month. At the end of the treatment, Bronchoalveolar lavage (BAL) fluid was collected and labelled with CD3e FITC, Ly-6G (Gr-1) PE-Cy-7, Allergin-1 eFlour® 660, CD14 APC and 2',7'-dichlorofluorescein diacetate (20 μM) and analyzed by flow cytometer (BD FACSCanto™ II) for cellular infiltration and reactive oxygen species (ROS) generation. There was a significant reduction in neutrophil infiltration following CP oil treatment for one month ( 68±6% vs 40±7%, P < 0.05). Furthermore, there was a significant decrease in ROS generation following CP oil treatment for one week (5873 ± 1133 vs 2581 ± 1359; P < 0.05) and one month (20618 ± 1854 vs 5850 ± 1006; P < 0.05). Further, theophylline treatment for one month reduced the ROS generation significantly (20618 ± 1854 vs 5286 ± 2413; P < 0.05). Conclusion: Celastrus paniculatus seed oil seems to reduce oxidative stress by inhibiting the generation of reactive oxygen species in LPS-induced lung inflammation.

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