Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2021  |  Volume : 17  |  Issue : 75  |  Page : 565-577

Atractylodis macrocephalae rhizoma decoction and its chemically profiled subfractions alleviate the side effects of rhubarb in TCM pair medicine


1 Department of Chinese Medicine Chemistry, College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China
2 Department of Pharmacology, School of Pharmacy, North West University, South Africa
3 Department of Pharmacy, Shaheed Benazir Bhutto University, Khyber Pakhtunkhwa, Pakistan

Correspondence Address:
De-Qiang Dou
College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600
China
Muhammad Riaz
Department of Pharmacy, Shaheed Benazir Bhutto University, Khyber Pakhtunkhwa
Pakistan
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_97_21

Rights and Permissions

Background: Pair medicine is a unique feature of Chinese medicine. It refers to a specific composition of the two drugs usually used in the formulation of composite medicine, which could increase synergistically or decrease the side effects of one medication. Atractylodis macrocephalae Rhizoma (AMR), a generally used drug, possessed the spleen-tonifying action always used with Rhubarb as a pair medicine to decrease the side effect of Rhubarb. Aim: To investigate the effect of Atractylodis macrocephalae Rhizoma (AMR) and its effective constituents to alleviate the Rhubarb's side effect in their pair medicine. Materials and Methods: The mice were administered Rhubarb until the induction of diarrhea followed by gastrointestinal injury. The gastrointestinal injured mice were treated with AMR's water decoction and its subfractions for 5 consecutive days. The decoction and subfraction were chemically characterized using the High-performance liquid chromatography (HPLC) with a diode-array detector (DAD) analysis. Results: The results showed that AMR's water decoction (6 g/kg) was discovered to be the most effective dose to treat gastrointestinal injury induced by Rhubarb. Body weight, thymus and spleen indexes, the intestinal propulsion rate, and D-xylose absorption in mice with diarrhea and intestinal injury were analyzed to reveal the significant difference between the tested and control groups (P < 0.01). The Water Eluated Fraction (WEF), Petroleum Ether Fraction (PEF), and Crude Polysaccharide Fraction (CPF) could not only increase the levels of amylase, gastrin, and vasoactive intestinal peptide significantly but also ease diarrhea and intestinal injury situation compared with the control group (P < 0.01). Conclusion: AMR and its subfractions effectively ameliorate gastrointestinal side effects of Rhubarb and its components. WEF mainly contained 5-hydroxymethyl furfural and small molecular sugar. PEF mainly composed of sesquiterpene lactone-atractylenolides, while CPF mainly composed of inulin-type oligosaccharides elucidated by HPLC analysis, was the most effective subfraction to alleviate diarrhoea and gastrointestinal injury induced by Rhubarb.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed110    
    Printed0    
    Emailed0    
    PDF Downloaded20    
    Comments [Add]    

Recommend this journal