|Year : 2021 | Volume
| Issue : 75 | Page : 468-474
Comparison of seven chemical components in Callicarpa nudiflora from different regions by liquid chromatography quadrupole time of flight mass spectrometry and its analgesic effect
Yunlu Peng1, Yuanyan Hu2, Lin Sheng3, Jianping Tian3, Jianlei Zhang4
1 School of Pharmacy, Hainan Medical University, Haikou, China
2 College of Ocean , Hainan University, Haikou, China
3 School of Pharmacy, Hainan Medical University; Hainan Key Laboratory for Research and Development of Tropical Herbs; Key Laboratory of Tropical Translational Medicine of Ministry of Education, Haikou, China
4 Qingdao Scitech Innovation Quality Testing Co.Ltd., Qingdao, China
|Date of Submission||17-Aug-2020|
|Date of Decision||12-Oct-2020|
|Date of Acceptance||12-Mar-2021|
|Date of Web Publication||11-Nov-2021|
School of Pharmacy, Hainan Medical University, Haikou, Hainan Provinces
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Callicarpa nudiflora has been usually employed as a traditional Chinese medicine for acesodyne, dispersing edema, and hemostasis; however, its analgesic effects have been poorly considered. Objectives: This article focused on the differences in the contents of seven active chemical constituents in C. nudiflora grown in different regions. Moreover, we examined the analgesic effect of C. nudiflora from five arbitrarily designated regions and studied the relationship between the compound contents and analgesic effect. Materials and Methods: A simple and delicate qualitative tandem liquid chromatography quadrupole time of flight mass spectrometry method was first established for the immediate determination of seven active components (luteolin, apigenin, quercetin, oleanolic acid, ursolic acid, protocatechuic acid, and caffeic acid) in C. nudiflora. Ethanol extracts were prepared from the mature leaves of C. nudiflora from five regions and the writhing test was performed by intragastric administration in mice. SPSS 13.0 and Graph Pad Prism 8.01 software were used to analyze the correlation of all data. Results: Calibration curves presented satisfactory linearity, with correlation coefficients >0.99 for all compounds within the concentration range. The compound contents were uppermost in plants from Hainan Province. The contents of the seven active chemical components inclined in the order of caffeic acid > luteolin > apigenin > oleanolic acid > ursolic acid > protocatechuic acid > quercetin. Pharmacological studies showed that C. nudiflora from all five regions had obvious analgesic effects. Conclusion: These results might be helpful for the screening and cultivation of C. nudiflora and its realistic clinical application.
Keywords: Analgesic effect, Callicarpa nudiflora, determination, liquid chromatography quadrupole time of flight mass spectrometry, writhing test
|How to cite this article:|
Peng Y, Hu Y, Sheng L, Tian J, Zhang J. Comparison of seven chemical components in Callicarpa nudiflora from different regions by liquid chromatography quadrupole time of flight mass spectrometry and its analgesic effect. Phcog Mag 2021;17:468-74
|How to cite this URL:|
Peng Y, Hu Y, Sheng L, Tian J, Zhang J. Comparison of seven chemical components in Callicarpa nudiflora from different regions by liquid chromatography quadrupole time of flight mass spectrometry and its analgesic effect. Phcog Mag [serial online] 2021 [cited 2022 Jul 7];17:468-74. Available from: http://www.phcog.com/text.asp?2021/17/75/468/330210
- The existing study reports the quantitative fortitude of luteolin, apigenin, quercetin, oleanolic acid, ursolic acid, protocatechuic acid, and caffeic acid present in the ethanol crude extract from Callicarpa nudiflora using liquid chromatography quadrupole time of flight mass spectrometry. The ethanol extract of the leaves of C. nudiflora was evaluated for their analgesic effect. There were significant differences in the contents of C. nudiflora and the analgesic effect may have an optimistic connection with the content of active ingredients.
Abbreviations used: C. nudiflora: Callicarpa nudiflora; LC-QTOF MS/MS: Liquid chromatography quadrupole time of flight mass spectrometry; TCM: Traditional Chinese medicine; HPLC: High-performance liquid chromatography; LOD: Limit of detection; LOQ: Limit of quantitative.
| Introduction|| |
Callicarpa nudiflora, a member of the Verbenaceae family, is largely distributed in Hainan, Guangdong, and Guangxi provinces of the P. R. of China. The dried mature leaves of this plant (C. nudiflorae Folium) are employed as a medicine, and this plant was designated as a new variety of traditional Chinese medicine (TCM) by the 2015 edition of the Chinese Pharmacopoeia. Similar to Yunnan white medicinal powder, which is a renowned hemostatic medicine in Yunnan Province, P. R. China, C. nudiflora is often called Hainan black medicine because its leaves often turn black after drying. Modern pharmacological studies display that C. nudiflora and its preparation C. nudiflora tablets unveil a diversity of pharmacological effects, such as anti-inflammatory, hemostatic, burn- and scald-relieving effects. Phytochemical research specifies that C. nudiflora encompasses many constituents, such as flavonoids, terpenoids, and volatile oils and so on. However, as there are diverse growth environments and active constituents in different C. nudiflora cultivation regions, the quality of crude drugs is also different. It is difficult to comprehend the standardization of commercial crude drugs. Earlier studies have engrossed on the chemical constituents and quality control of C. nudiflorae Folium from different areas: High-performance liquid chromatography (HPLC) analysis, determination of different components, and establishment of HPLC fingerprints. The total flavonoids and verbascoside contents in the same genus of Callicarpa L. are detected as the key indicators of quality control, but the existing methods lack specificity and the chemical components of TCM studied are comparatively modest and cannot imitate the difference in chemical composition contents of C. nudiflorae Folium from different regions. None of the seven active chemical components stated in this article has been described yet. In addition, the drug has been confirmed to have good analgesic effects in the clinical applications; however, few studies have focused on the analgesic effect of C. nudiflorae Folium.
The components of plant metabolites in extracts can be expansively and precisely analyzed, by using the technology of plant metabonomics, particularly for the analysis of multi-component complex systems, which promotes research with a holistic view of TCM., In the present study, the leaves of C. nudiflora were composed from different regions and a simple and delicate qualitative tandem liquid chromatography quadrupole time of flight mass spectrometry (LC-QTOF MS/MS) method was first established for the immediate determination of luteolin, apigenin, quercetin, oleanolic acid, ursolic acid, protocatechuic acid, and caffeic acid in a C. nudiflora extract. In addition, this article examined the analgesic effect of C. nudiflora grown in five arbitrarily selected regions and studied the relationship between the compound contents and the analgesic effect. It was predictable that the results acquired would deliver some references to gauge the screening, cultivation, and the clinical application of C. nudiflora.
| Materials and Methods|| |
An ultrasonic unit (B2500S-MT), a diode array detector with a binary high-voltage gradient pump (American Waters Co.), a vacuum pump (DOA-P730-BN), a vacuum dryer (Shanghai BoXun Medical Equipment Factory), a high throughput API 3200 LC/MS/MS system (American Waters Co.), and tandem triple quadrupole linear ion trap mass spectrometer (American Allen-Bradley Co.) were employed in this study.
Chemicals and reagents
Standards of the seven compounds were acquired and were of HPLC grade (≥98% purity, Nanjing Senbeijia Reagent Co.). The information is revealed in [Table 1]. The extraction solvents were 95% ethanol, methanol and anhydrous acetic acid (analytical purity).
|Table 1: The seven compounds in Callicarpa nudiflora simultaneously analyzed by tandem liquid chromatography quadrupole time of flight mass spectrometry|
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The species employed in this study was recognized as C. nudiflora by Professor Tian Jianping, School of Pharmacy, Hainan Medical University, P. R. China specimen was dropped in the Herbarium of Hainan Medical University. In total, twenty samples of C. nudiflorae Folium used for content determination were collected in Hainan, Guangdong, and Guangxi provinces from 2018 to 2019. The particulars are shown in [Table 2]. The samples employed for the study of analgesic effect were Nos. 6, 9, 12, 13, and 16 in [Table 2]. All samples were desiccated at 50°C, creased into fine powder, sieved through 0.45 mm mesh, placed in a wide-mouth bottle, sealed, and stored in a cool place until succeeding analysis.
|Table 2: Comprehensive information for the twenty samples of Callicarpa nudiflora folium studied|
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Liquid chromatography quadrupole time of flight mass spectrometry analytical conditions
The chromatographic conditions were as trails: A C18 column (100 mm × 2.1 mm, 3.5 μm) was used, methanol was used as mobile phase A and aqueous 5 × 10−3 mL/min formic acid was used as mobile phase B. The gradient elution programme was as follows: 0 min, 25% A; 7 min, 80% A and 7.01–9.0 min, 25% A. The flow rate was 0.4 mL/min, the column temperature was 40°C, the exposure wavelength was 280 nm, and the injection volume was 2.0 μL.
The mass spectrometric circumstances were as trails: The electrospray ionization source was worked in negative ion mode, the electrospray scanning voltage was set at −4500 V, the ion source temperature (TEM) was 550°C, and the ion source auxiliary pressure was 1369 kPa.
Preparation of standard solutions
The reference compounds caffeic acid, apigenin, protocatechuic acid, oleanolic acid, ursolic acid, quercetin, and luteolin at masses of 1.5, 1.5, 2.0, 2.0, 3.0, 3.5, and 4.0 mg were considered and liquefied in 70% methanol in a 5 mL volumetric flask to yield the stock solutions (0.3, 0.3, 0.4, 0.4, 0.6, 0.7, and 0.8 mg/mL, respectively). The stock solutions were diluted to suitable concentrations by adding 70% methanol for LC-QTOF MS/MS analyses. All solutions were deposited at 4°C and kept away from light.
Preparation of sample solution
C. nudiflora powder (1.0 g) from unlike cultivation regions (sample Nos. 1–20) was weighed put into a 50 mL volumetric flask, mixed with 70% methanol until dissolved and ultrasonically extracted (power: 250 W and frequency: 42 kHz) for 30 min. Due to the change in ultrasonic intensity at diverse positions of the ultrasonic instrument, the position of the conical bottle was staggered during the treatment process. The supernatant was composed and filtered with a 0.22 μm porous membrane filter. Finally, the supernatants (20 μL) were transferred into glass vials before injection into the LC-QTOF MS/MS for examination.
Preparation of Callicarpa nudiflora extract
The preparation of C. nudiflora extract was acquired according to the methodology planned by Shao et al. with slight variations. Dried leaf powder of C. nudiflora (1 kg) was extracted three times (1 h/each) with 95% alcohol. The extract was combined and freeze vacuum drying for 24 h to attain the extract of C. nudiflora.
Method validation of determination
The method was authenticated according to the fourth part of the Pharmacopoeia of the People's Republic of China (2015 Edition). The following parameters were dogged: Precision, recovery, linearity, limit of detection (LOD), and limit of quantitation (LOQ). Endorsement was conducted following the procedure of Han, Rui and Ying with slight amendments.,,
According to the above chromatographic conditions, the mixed reference solution was precisely absorbed, the sample was inoculated incessantly for 6 times, the peak area of each component was documented, performed on different days, with different analysts, but with the same equipment. The results were articulated by relative standard deviation (RSD) value.
C. nudiflora powder (1.0 g) from sample number 1 was evaluated put into a 10 mL volumetric flask, and the mixed reference solution with diverse mass concentration was added 1 mL, respectively. The recovery experiment of standard addition was performed with the same operation stages and experimental conditions as preparation of sample solution. The peak area was assessed and recorded, and the recovery rate was considered.
Linearity, limit of detection, and limit of quantitation
An appropriate amount of prepared standard solution was located into a conical flask, and methanol solution was added and mixed well. The mass concentrations of the standard solutions were 5 μg/L, 10 μg/L, 25 μg/L, 50 μg/L, 1100 μg/L, and 250 μg/L. After the peak areas of each reference substance were dogged, the regression equations were considered. The LOD was the compound concentration yielding a signal-to-noise (S/N) ratio of 3:1 and the LOQ was the concentration yielding an S/N ratio of 10:1. Lastly, the contents of chemical components in separate samples were calculated, and the relevant data were administered by SPSS 13.0 (Bontz Inc., Beijing, China).
Kunming mice were delivered by Hunan Slyk Jingda Experimental Animal Limited Company, and the production licence number of experimental animals is SCXK (Xiang) 2019-0004. This study was directed in accordance with the Experimental Animal Administration regulations issue by the State Committee of Science and Technology of the People's Republic of China. All procedures defined here had prior approval from the Institutional Animal Care and Use Committee at the Hainan Medical University (Haikou, China).
Acetic acid-induced writhing in mice
Dry extracts of five samples were diluted with normal saline and ultrasonic suspensions were organized for the animal study. Sixty healthy male and female mice house at a room temperature of 18°C and weighing 18–22 g were arbitrarily alienated into one control group (normal saline group) and five experimental groups (ten mice in each group). In the experimental groups, the mice were intragastrically administered extracts of C. nudiflora (0.2 mL/10 g). After 30 min, 0.6% acetic acid (0.2 mL/10 g) was intraperitoneally injected to induce pain. The number of writhing mice and writhing times in 20 min were calculated. The inhibition rate of the writhing reaction was gained. The results were statistically examined by the SPSS software version 13.0.
| Results and Discussion|| |
Optimization of liquid chromatography quadrupole time of flight mass spectrometry parameters
The conformation of the mobile phase is very vital in the selection of chromatographic conditions and controls the ionization efficiency of the samples to be assessed. The results display that the concentration of formic acid can affect the ionization efficiency, but it had no palpable effect on the separation of the components to be dogged. The impact on data of the specific concentration was as trails: When the concentration of formic acid was 2.0 mmol/L, the ionic strength of each index in the total ion flow diagram of the mixed standard sample was 1.0 × 105 and the signal was comparatively low. When the concentration of formic acid was 5.0 mmol/L, the ionic strength was 1.0 × 107, which revealed that the ionization efficiency of the mixed standards was knowingly developed. However, when the concentration of formic acid in the mobile phase was 10.0 mmol/L, the ionic strength was 3.4 × 107 and the signal augmented. Compared with the methanol system, the acetonitrile/water system had no obvious effect on the resolution and ionization efficiency of chromatographic peaks. Considering that the toxicity of methanol is less than that of acetonitrile, methanol was nominated for use as mobile phase A. Considering the economy and ionization efficiency, an aqueous solution containing 5.0 mmol/L formic acid was lastly designated as mobile phase B. According to the references and experimental testing, the ideal gradient conditions were designated as follows: 0 min, 25% A; 7 min, 80% A and 7.01–9.0 min, 25% A.
Qualitative analysis of Callicarpa nudiflora
The LC-QTOF MS/MS chromatograms of the seven reference standards and twenty samples are revealed in [Figure 1] and [Figure 2]. Under the detection conditions employed in this experiment, the retention times of the seven compounds were all in the range of 1.4–6.14 min, with good peak shape, no intrusions from impurities were detected and the baseline was steady. The method had high specificity, could precisely assess the content of seven chemical constituents in the mixed sample of C. nudiflora and had high sensitivity.
|Figure 1: Selective ion chromatography of mixed reference substance of Callicarpae nudiflorae Folium. The mass spectrum information of reference substance is on the rightof the graph. From top to bottom are protocatechic acid, caffeic acid, apigenin, luteolin, quercetin, ursolic acid and oleanolic acid|
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|Figure 2: Selective ion chromatography of mixed samples of Callicarpae nudiflorae Folium. The mass spectrum information of reference substance is on the right of the graph. From top to bottom are protocatechic acid, caffeic acid, apigenin, luteolin, quercetin, ursolic acid and oleanolic acid|
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Ion pair chromatography can effectually separate difficult-to-separate mixtures, as shown in [Figure 3], which is the extractive ion pair chromatogram attained in multiple reaction monitoring mode for numerous indicators to be assessed in the extract of C. nudiflora. According to the analysis of [Figure 3], it could be realised that all seven targeted chemical components were well detached and the run time was shorter than those of other methods. Separation and detection could be finished in a short time of 3.5 min.
|Figure 3: Extraction ion flow in multistage reaction mode of Callicarpae nudiflorae Folium|
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Method validation of Callicarpa nudiflora
Method validation studies disclosed that the linear regression equations, linear ranges, LODs and LOQs of oleanolic acid, ursolic acid, caffeic acid, luteolin, protocatechuic acid, quercetin and apigenin are presented in [Table 3]. The repeatability RSDs of all seven compounds in the sample solution did not surpass 2%. The low RSD values advise that the test procedure had good repeatability. The recovery rates (R%) of the seven compounds in the validation study demonstrated that the method was consistent when analyzing the seven compounds in C. nudiflora. The accuracy RSDs did not outstrip 5%. The quantitative limit was 0.2–3.0 μg/L for an S/N ratio of 10:1 and the detection limit was 0.05–1.0 μg/L for an S/N ration of 3:1. The grades of the validation studies verified that the method was steadfast when analyzing the seven target compounds in C. nudiflora.
|Table 3: The calibration and validation data obtained for the quantification of the seven compounds|
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Comparison of seven chemical constituents in Callicarpa nudiflora from different regions
The contents of the compounds in the samples are accessible in [Table 4] and [Figure 4]. Comparing of the contents of the seven chemical elements in C. nudiflora grown in different regions, the contents inclined in the order of caffeic acid > luteolin > apigenin > oleanolic acid > ursolic acid > protocatechuic acid > quercetin. The consequences exposed that the luteolin and quercetin contents of C. nudiflora were the uppermost in plants from Hainan Province, followed by those from Guangdong and Guangxi. There were substantial differences in the contents of chemical components in plants grown in different regions. The quality of the crude drug also mottled with region. In clinical applications, the choice of the origin of crude drugs is very imperative. Compared with C. nudiflora plants grown in Hainan Province, those grown in Guangdong and Guangxi provinces presented inferior quality, which also follows to the fact that the producing area of authentic crude drugs of C. nudiflora was Hainan Province. In addition, the content of compounds in C. nudiflora grown in Shiyun village of Hainan Province was the finest, and the cultivation of high-quality varieties of C. nudiflora can be carried out in this area.
|Table 4: Comparison of seven chemical constituents in Callicarpae nudiflorae Folium from different areas (mg/g)|
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|Figure 4: Comparison of content of seven chemical constituents in Callicarpae nudiflorae Folium from different areas|
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Writhing reaction in mice
Inhibition rate = (mean number of writhing times in control group-average number of writhing times in medication group)/average number of writhing times in control group × 100%. The effect of the extract of C. nudiflorae Folium on the writhing reaction of mice tempted by acetic acid is presented in [Table 5] and [Figure 5]. The outcomes exhibited that there were noteworthy differences between the control and treated mice, with P values between P < 0.05 and P < 0.01 in one-way analysis of variance. The results also disclosed that the ethanolic extract of mature dried leaves of C. nudiflora had an obvious inhibitory effect on writhing encouraged by acetic acid in mice.
|Table 5: Effect of extract of Callicarpae nudiflorae folium on writhing reaction in mice induced by acetic acid|
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|Figure 5: Difference analysis of writhing reaction in mice. Control was the blank control group. A-E are derived from the No 6, 9, 12, 13, 16 samples in Table 2, respectively. Data are expressed as mean ± standard deviation (at column width). *P < 0.05, **P < 0.01 versus the control group|
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Earlier studies have exposed that flavonoids, such as rhododendron and Rutin, extracted from TCMs can ominously diminish the number of writhing reactions in mice., Myrrha is mostly composed of volatile oil and terpenoids, which can suggestively inhibit pain induced by acetic acid. It can be inferred that the analgesic effect of TCMs is primarily linked to their contents of flavonoids and terpenoids. To discover the analgesic effect of the active components of C. nudiflora from different areas, five samples in [Table 2] were aimlessly selected to prepare C. nudiflorae Folium extracts. In the writhing test of mice, it was found that all C. nudiflorae Folium extracts had inhibitory effects on the writhing reaction of mice induced by acetic acid, but the degree of inhibition was diverse. The inhibition rates for sample No. 9 were the lowest at 19.35%. According to the results, it was found that the contents of seven components in sample No. 9 were also the lowest compared with those in the other four samples. The grades established that the active chemical composition of C. nudiflorae Folium from twenty areas was altered and that the content directly affected its pharmacological action. This study further established the analgesic effect of C. nudiflorae Folium in clinical application and its pharmacodynamic material basis may be correlated to the contents of flavonoids and terpenoids.
| Conclusion|| |
In conclusion, the outcomes of the methodology validation were decent and the quantitative method employed in this experiment can be productively applied to the routine quality control of the leaves of C. nudiflora. There were substantial differences in the content of C. nudiflora in different regions. Among the five regions, Haikou City of Hainan province had the uppermost content of chemical constituents and the greatest analgesic effect. This study laid a groundwork for quality screening and cultivation of C. nudiflora.
We would like to express thanks to the staff and crew of the Hainan Medical University for their untiring efforts and perseverance.
Financial support and sponsorship
This research was supported by National Natural Science Foundation of China (81,660,714). The funders had no role in the study design and analysis.
Conflicts of interest
There are no conflicts of interests.
| References|| |
Editorial Committee of Flora of China. Flora of China. Vol. 65. Beijing: Science Press; 1982. p. 37.
National Committee of Pharmacopoeia. Pharmacopoeia of the People's Republic of China. Vol. 1. Beijing: People's Heath Publishing House; 2015. p. 1669.
Wang HG, Luo FK, Lei X, Yao YD, Liao GC, Liu ZQ, et al
. 3, 4-seco-Labdane diterpenoids from the leaves of Callicarpa nudiflora
with anti-inflammatory effects. Chin J Nat Med 2019;17:707-12.
Shao J, Ma S, Zheng D, Chen W, Luo Y. Simultaneous determination of four active components in rat plasma by ultra-high performance liquid chromatography tandem-mass spectrometry/mass spectrometry and its application to a pharmacokinetic study after oral administration of Callicarpa nudiflora
extract. Pharmacogn Mag 2015;11:509-17.
Zhang XG, Li XM, Zhou XX, Wang Y, Lai WY, Liu Y, et al
. The wound healing effect of Callicarpa nudiflora
in scalded rats. Evid Based Complement Alternat Med 2019;2019:1-8.
Liang JJ, Qi JL, Li L, Jing XJ, Wang ZY. Flavonoids from Callicarpa nudiflora
leaves. Chem Nat Compd 2011;47:110-1.
Zhou Z, Wei X, Fu H, Luo Y. Chemical constituents of Callicarpa nudiflora
and their anti-platelet aggregation activity. Fitoterapia 2013;88:91-5.
Liang JJ, Han F, Wang ZG, Yan Y, Mo FK. Chemical composition of the essential oil from leaves of Callicarpa nudiflora
. Chem Nat Compd 2009;45:267-8.
Ma SY, Lin S, Guan WW, Liu MS. HPLC fingerprint of Callicarpa nudiflora
. Zhong Yao Cai 2014;37:404-8.
Wu YS, Shi L, Liu XG, Li W, Wang R, Huang S, et al
. Chemical profiling of Callicarpa nudiflora
and its effective compounds identification by compound-target network analysis. J Pharm Biomed Anal 2020;182:110-3.
Li XM, Wang Y, Zhang JQ, Lai WY, Zhang XG. Simultaneous determination of three kinds of phenylethanoid glycosides in Callicarpa nudiflora
by HPLC method. Guangzhou Chem Ind 2019;47:104-6.
Tu Y, Sun L, Guo M, Chen W. The medicinal uses of Callicarpa
L. in traditional Chinese medicine: An ethnopharmacological, phytochemical and pharmacological review. J Ethnopharmacol 2013;146:465-81.
Feng SX, Zhang M, Yi B, Wu HX. Research progress on chemical constituents in Callicarpa nudiflora
and their pharmacological activities. Chin Tradit Herb Drugs 2017;48:1015-26.
Chavez Soria NG, Bisson MA, Atilla-Gokcumen GE, Aga DS. High-resolution mass spectrometry-based metabolomics reveal the disruption of jasmonic pathway in Arabidopsis thaliana
upon copper oxide nanoparticle exposure. Sci Total Environ 2019;693:133-44.
National Committee of Pharmacopoeia. Pharmacopoeia of the People's Republic of China. Vol. 4. Beijing: People's Heath Publishing House; 2015. p. 374.
Han L, Liu E, Kojo A, Zhao J, Li W, Zhang Y, et al
. Qualitative and quantitative analysis of Eclipta prostrata
L. by LC/MS. ScientificWorldJournal 2015;2015:890-910.
Shi R, Qiao S, Yu D, Shi X, Liu M, Jiang X, et al
. Simultaneous determination of five flavonoids from Scutellaria barbata
extract in rat plasma by LC-MS/MS and its application to pharmacokinetic study. J Chromatogr B Analyt Technol Biomed Life Sci 2011;879:1625-32.
Yang W, He S, Xiao N, Qiao Y, Sui H, Liang L, et al
. Simultaneous determination of 15 flavonoids in Scutellaria barbata-Hedyotis diffusa
Herb Pair by HPLC Q-TOF MS. J AOAC Int 2018;142:75-80.
Shi TT, Zhang XB, Guo LP, Huang LQ. Random forest classification of Callicarpa nudiflora
from WorldView-3 imagery based on optimized feature space. Zhongguo Zhong Yao Za Zhi 2019;44:4073-7.
Zheng DS, Ji H, Hu QH. Advances in research of traditional Chinese analgesic medicine. Chin J New Drugs 2017;26:782-6.
Cheng HL. Advances in the effective components on analgesia by traditional Chinese medicine. J Anhui Sci Tech Univ 2012;26:11-4.
Su S, Wang T, Duan JA, Zhou W, Hua YQ, Tang YP, et al
. Anti-inflammatory and analgesic activity of different extracts of Commiphora myrrha
. J Ethnopharmacol 2011;134:251-8.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5]
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5]