Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
Advanced search 
Year : 2021  |  Volume : 17  |  Issue : 6  |  Page : 225-232

Effect of salinity stress on growth, water content, and guggulsterone production in callus cultures of Commiphora wightii (Arnott.) Bhandari

1 Dabur Research and Development Centre, Ghaziabad, Uttar Pradesh, India
2 Department of Botany, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India
3 National Medicinal Plant Board (NMPB), Ministry of Ayurveda, Yoga and Naturopathy, Unani, Siddha and Homeopathy (AYUSH), Government of India, New Delhi, India
4 Department of Pharmacognosy and Phytochemistry, Bioactive Natural Product Laboratory, SPER, Jamia Hamdard, New Delhi, India

Correspondence Address:
Sayeed Ahmad
Department of Pharmacognosy and Phytochemistry, Bioactive Natural Product Laboratory, SPER, Jamia Hamdard, New Delhi - 110 062
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/pm.pm_514_20

Rights and Permissions

Objectives: A traditional herb Commiphora wightii (Arnott.) is well known for the management of different ailments, including hypolipidemic and hypocholesterolemic disorders. Overexploitation has resulted in enlisting it under endangered species list; thus, in vitro studies are desirable to circumvent further depletion of the wild population. The objective of our study is to analyze the effect of salinity stress on growth and guggulsterone content in callus cultures. Materials and Methods: The establishment of leaf-derived calli of C. wightii and the effect of salinity stress such as calcium chloride (CaCl2), sodium chloride (NaCl), potassium chloride, and ammonium sulfate at different concentrations and time periods on callus growth and steroid biosynthesis were studied. High-performance liquid chromatography and liquid chromatography-mass spectrometry/mass spectrometry-based spectrometric systems were carried to identify the guggulsterones. Results: The 50 mg/L CaCl2-induced callus was found to have the highest content of guggulsterones after 15 months of incubation as compared to 9 and 12 months and indicated a positive correlation between callus age and guggulsterone biosynthesis. Conclusion: Salinity stress played an important role in callus growth of C. wightii, while calcium-induced calli showed in vitro synthesis of guggulsterones, and this can be further explored for the synthesis of guggulsterones in vitro on large commercial scale using bioreactor, and thus helps to conserve the endangered species in its natural habitat.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded143    
    Comments [Add]    

Recommend this journal