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Year : 2020  |  Volume : 16  |  Issue : 67  |  Page : 192-196

Red seaweed (Eucheuma cottonii) extract promotes human keratinocyte migration via p38 mitogen-activated protein kinase phosphorylation

1 Department of Biochemistry, Chung-Ang University College of Medicine, Dongjak-gu, Seoul, Korea
2 Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia

Correspondence Address:
Dong-Seok Kim
Department of Biochemistry,Chung-Ang University College of Medicine, 84 Heukseok-ro, Dongjak-gu, Seoul 06974
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/pm.pm_203_19

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Background: Eucheuma cottonii (EC) is a rapidly growing red seaweed in Southeast Asia. Objectives: This study was performed to investigate the effects of EC extract on human keratinocyte migration. Materials and Methods: Cell migration was assessed using the scratch wound assay. Western blot analysis was performed to investigate the EC extract-induced signaling pathways. Results: EC extract promoted HaCaT keratinocyte migration in a concentration-dependent manner. To investigate the mechanism of EC extract-induced migration, we examined the migration-related signaling pathways. Western blot analysis showed that the EC extract showed no changes in extracellular signal-activated kinase (ERK) but showed slight Akt activation. In contrast, the EC extract strongly phosphorylated p38 mitogen-activated protein kinase (MAPK) and the subsequent downstream molecule, cAMP response element-binding protein (CREB). To examine the involvement of p38 MAPK pathway in EC extract-induced migration, SB203580, a specific inhibitor of p38 MAPK, was used. The results showed that EC extract-induced migration was abrogated by SB203580 pretreatment. In addition, SB203580 also blocked p38 MAPK and CREB activation. Conclusion: Taken together, these data suggest that EC extract promotes migration in human keratinocytes via p38 MAPK and CREB phosphorylation.

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