ORIGINAL ARTICLE

Year : 2020  |  Volume : 16  |  Issue : 5  |  Page : 566-572

Protective effect of Viburnum grandiflorum against ultraviolet-B radiation-induced cellular and molecular changes in human epidermal keratinocytes

Hanjun Liu¹, Haixiu Zhang², Minyan Dang³, Yukiat Lin³, Hui Yan^4
1 Department of Dermatology, Hanzhong People's Hospital, Hanzhong, Shaanxi 723000, China
² Department of Dermatology, Jinan Municipal Hospital of Traditional Chinese Medicine, Jinan, Shandong 250012, China
³ Innoscience Research Sdn Bhd, Selangor, Malaysia
⁴ Department of Dermatology, The Second People's Hospital of Yunnan Province, Kunming, Yunnan 650021, China

Correspondence Address:
Hui Yan
Department of Dermatology, The Second Peoplefs Hospital of Yunnan Province, Kunming, Yunnan 650021
China

Source of Support: None, Conflict of Interest: None

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DOI: 10.4103/pm.pm_397_19

Abstract

The aim of the present study was to evaluate the photoprotective potential of Viburnum grandiflorum (VG) against ultraviolet-B radiation-induced responses in HaCaT cells. The HaCaT cells were pretreated with VG prior ultraviolet-B (UVB)-radiation exposure and were further examined for lipid peroxidation, enzymatic antioxidant activity, % reactive oxygen species, DNA damage, mitochondrial membrane potential and for inflammatory, and apoptotic signaling markers such as tumor necrosis factor-alpha, nuclear factor kappa B, interleukin-1 (IL-1), IL-6, cyclooxygenase-2, p53, caspase-3/9, cytochrome-c, Bax, and Bcl-2. The VG pretreatment in UVB exposed cells shows significantly regulated both inflammatory as well as apoptotic signaling cascades. Our findings suggest that VG may be functional against UVB-induced photo-damages.

Keywords: Apoptosis, inflammation, mitochondrial membrane potential, ultraviolet-B radiation, Viburnum grandiflorum