Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
Advanced search 
Year : 2018  |  Volume : 14  |  Issue : 59  |  Page : 535-538

Tropidia Curculioides: Secondary metabolites and derivatives with antimycobacterial and leishmanicidal activity

1 Centre for Plant and Environmental Biotechnology, Amity Institute of Biotechnology, AUUP, Noida, Uttar Pradesh, India
2 Department of Clinical Microbiology and Laboratory Medicine, AIIMS, New Delhi, India

Correspondence Address:
Sabari Ghosal
Centre for Plant and Environmental Biotechnology, Amity Institute of Biotechnology, AUUP, Noida - 201 303, Uttar Pradesh
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/pm.pm_196_18

Rights and Permissions

Background: Arunachal Pradesh the north-eastern state of India is a natural habitat of 550 species of orchids including 37 species of medicinal importance. Although these plants are regularly used by ethnic population, very few scientific investigations have been conducted to establish their pharmacological potential. In our previous study, we evaluated antimycobacterial and leishmanicidal activity of extracts prepared from three relatively unexplored medicinal orchids. In the present study, we conducted screening of the compounds isolated from Tropidia curculioides (Tc) for the above-mentioned activities. Furthermore, we prepared synthetic analogs of the most active compound. Objective: Evaluation of antimycobacterial and leishmanicidal activity of the isolated compounds and preparation of synthetic analogs of the most active compound followed by evaluation of biological activities. Materials and Methods: Antimycobacterial activity was evaluated by colorimetric redox indicator assay, and the leishmanicidal activity was assessed by 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide assay. The root, stem, and leaves of Tc were extracted with methanol:water (9:1) followed by fractionation with diethyl ether (Et2O) and n-butanol solvent. All these fractions were evaluated for biological activity to identify the most active fraction. Further, chromatography of the most active fraction (Tc root Et2O fraction) afforded three compounds, namely, 4-hydroxybenzaldehyde (1), 4,4'-dihydroxydiphenylmethane (2), and 3,5-dihydroxy-4-methoxybenzoic acid (3). Standard synthetic procedures were followed to prepare the analogs of the most active compound. Results: The screening result identified compound 2 with maximum antimycobacterial activity (minimum inhibitory concentration [MIC] – 125 μg/ml) and leishmanicidal activity (IC50 100 μg/ml). Three synthetic analogs were prepared targeting the methane linkage of compound 2. The N-benzylmethylamine derivative (6) showed the highest activity with MIC 15.62 μg/ml against Mycobacterium and IC50 31.25 μg/ml against Leishmania spp. Conclusion: The promising result of N-benzylmethylamine analog could be explored to obtain new antimycobacterial and leishmanicidal agent. Abbreviations used: MIC: Minimum inhibitory concentration; NMR: Nuclear magnetic resonance; CRI: Colorimetric redox indicator; OADC: Oleic acid, dextrose, and catalase; MDR: Multidrug Resistant; MTT: 3-(4,5-dimethylthiazol-2-Yl)-2, 5-diphenyltetrazolium bromide; Diethyl ether: Et2O.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded159    
    Comments [Add]    
    Cited by others 1    

Recommend this journal