Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
Advanced search 
Year : 2018  |  Volume : 14  |  Issue : 55  |  Page : 161-166

Pharmacokinetic study on piplartine and piperine after oral administration of Piper chaba root by liquid chromatography-mass spectrometry/mass spectrometry

1 Division of Natural Products Chemistry, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, India
2 Department of Pharmaceutical Analysis, A.U. College of Pharmaceutical Sciences, Andhra University, Vishakapatnam, Andhra Pradesh, India
3 Division of Medicinal Chemistry and Biotechnology, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, India

Correspondence Address:
Suresh Babu Katragadda
Division of Natural Products Chemistry, CSIR-Indian Institute of Chemical Technology, Hyderabad - 500 007, Andhra Pradesh
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/pm.pm_470_17

Rights and Permissions

Background: Piperaceae family are a well-known source of structurally diverse amides with the wide range of bioactivities such as cytotoxic, stomach aches, insect repellents, anti-inflammatory, insecticidal, and antifeedant activities. It has been reported that piplartine and piperine, alkaloid/amide compounds from Piper species, show antitumor activities. Objective: A rapid, sensitive liquid chromatography-tandem mass spectrometry method has been developed and validated for the determination of piplartine and piperine from Piper chaba extract. Materials and Methods: The two analytes, together with internal standard (IS, trichostachine), were separated on a Waters Acquity ethylene bridged hybrid C18(2.1 mm × 100 mm, 1.9 μ) column using a mobile phase of acetonitrile with 0.1% formic acid and water with 0.1% formic acid (70:30, v/v) with isocratic elution. The detection was performed using the positive ion electrospray ionization in multiple reaction monitoring mode with transitions at m/z 318→221 for piplartine, m/z 286→201 for piperine, and m/z 272→201 for the IS. Results: The calibration curves were both linear (r2 > 0.995) over a concentration range of 1.0–2000 ng/mL; the lower limit of detection quantification was 1.0 ng/mL for both piplartine and piperine. The intra-day and inter-day precisions (relative standard deviation %) were <10.9%, and recoveries ranged from 90.3% to 103.0%. Conclusions: The analytes were proven stable in the short-term, long-term, and after three freeze-thaw cycles. The method was successfully applied to pharmacokinetic studies of piplartine and piperine in rats after oral administration of P. chaba extract. Abbreviations used: AUC: Area under the curve; BEH: Ethylene bridged hybrid; CDER: Centre for drug evaluation and research; CID: Collision-induced dissociation; Cmax: Maximum concentration; CTO: Column Temperature Oven; DGU: Degassing Unit; ESI: Electrospray ionization; eV: Electron volt; FCV: Flow control valve; HPLC: High-pressure liquid chromatography; HPTLC: High performance thin layer chromatography; IS: Internal standard; LLOQ: Lower limit of quantitation; LC: Liquid chromatography; LC-MS: Liquid chromatography-Mass Spectrometry; LC-MS/MS: Liquid chromatography-Mass Spectrometry/Mass Spectrometry; LC-HRMS: Liquid chromatography-High resolution mass Spectrometry; LC-NMR-MS: Liquid chromatography-Nuclear magnetic resonance-Mass Spectrometry; MRM: Multiple reaction monitoring; MC: Methyl cellulose; N2: Nitrogen; RSD: Relative standard deviation; RE: Relative error; r2: Regression coefficient; t1/2: Half-life; Tmax: Time to maximum effect; QC: Quality control; UFLC: Ultrafast liquid chromatography; UPLC-qTOF-MS: Ultra pressure liquid chromatography-Time of flight-Mass spectrometry; USFDA: United states Food and Drug Administration

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded271    
    Comments [Add]    
    Cited by others 2    

Recommend this journal