|
Brazilian cerrado Qualea grandiflora Mart. leaves exhibit antiplasmodial and trypanocidal activities In vitro
Thuany de Moura Cordeiro1, Fabian Borghetti2, Sarah C Caldas Oliveira3, Izabela Marques Dourado Bastos4, Jaime Martins de Santana4, Philippe Grellier5, Sébastien Charneau1
1 Department of Cell Biology, Laboratory of Biochemistry and Protein Chemistry, Institute of Biology, University of Brasilia, Darcy Ribeiro Campus, 70910-900, Brasilia, DF, Brazil
2 Department of Botany, Laboratory of Thermobiology, Institute of Biology, University of Brasilia, Darcy Ribeiro Campus, 70910-900, Brasilia, DF, Brazil
3 Department of Botany, Laboratory of Allelopathy, Institute of Biology, University of Brasilia, Darcy Ribeiro Campus, 70910-900, Brasilia, DF, Brazil
4 Department of Cell Biology, Laboratory of Host-Pathogen Interaction, Institute of Biology, University of Brasilia, Darcy Ribeiro Campus, 70910-900, Brasilia, DF, Brazil
5 UMR 7245 CNRS, Communication Molecules and Adaptation of Microorganisms, CP 52, 61 rue Buffon, 75231 PARIS CEDEX 05, France
Correspondence Address:
Sébastien Charneau
University of Brasília, Darcy Ribeiro Campus, Brasília-DF, 70910-900
Brazil
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/pm.pm_100_17
|
|
Background: The rapid spread of drug-resistant strains of protozoan parasites required the urgent need for new effective drugs. Natural products offer a variety of chemical structures, which make them a valuable source of lead compounds for the development of such new drugs. Cerrado is the second largest biome in Brazil and has the richest flora of all the world savannahs. We selected Qualea grandiflora, a plant species known for its proprieties in folk medicine and its antibacterial activity. Objective: However, its antiprotozoal activity was not yet explored. Materials and Methods: We investigated the activities of fractions from the ethyl acetate extract of Q. grandiflora leaves against human life forms of Plasmodium falciparum, Trypanosoma cruzi, and Trypanosoma brucei gambiense, and for its cytotoxicity upon the rat L6-myoblast cell line. Ten fractions were produced by ethyl acetate:hexane chromatography. Results and Conclusion: The fractions showed no cytotoxicity against L-6 cells (IC50 > 100 μg/mL) and no hemolysis propriety. Three fractions had a moderate activity against P. falciparum, anyone was active against T. cruzi but four fractions demonstrated a high activity against bloodstream forms of T. brucei gambiense (8.0< IC50 <15 μg/mL). Identification and characterization of the active compounds are currently under investigation.
Abbreviations used: CQ: Chloroquine, DMSO: Dimethyl sulfoxide, HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, HMI: Modified Iscove's medium, IC50: Concentration inhibiting 50% of parasite growth, IC90: Concentration inhibiting 90% of parasite growth, MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, RPMI: Roswell Park Memorial Institute, SD: Standard deviation, SI: Ratio of cytotoxicity to biological activity − TC50/IC50, TC50: Concentration causing 50% of cell growth inhibition, TC90: Concentration causing 90% of cell growth inhibition, TLC: Thin-layer chromatography
|
