| ORIGINAL ARTICLE |
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| Year : 2015 | Volume
: 11
| Issue : 42 | Page : 320-326 |
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Quantitative analysis of the eight major compounds in the Samsoeum using a high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometer
Jin Bae Weon1, Hye Jin Yang1, Bohyoung Lee1, Jin Yeul Ma2, Choong Je Ma3
1 Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea
2 TKM Converging Research Division, Korea Institute of Oriental Medicine, 483 Exporo, Yuseong gu, Daejeon 305-811, Korea
3 Department of Biomaterials Engineering, Division of Bioscience and Biotechnology; Research Institute of Biotechnology, Kangwon National University, Chuncheon 200-701, Korea
Correspondence Address:
Choong Je Ma
Department of Biomaterials Engineering, Division of Biotechnology and Bioengineering, Kangwon National University, Hyoja 2 Dong, Chuncheon 200-701
Korea
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0973-1296.153085
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Background: Samsoeum was traditionally used for treatment of a respiratory disease. Objective: The simultaneous determination of eight major compounds, ginsenoside Rg3, caffeic acid, puerarin, costunolide, hesperidin, naringin, glycyrrhizin, and 6-gingerol in the Samsoeum using a high-performance liquid chromatography (HPLC) coupled with diode array detection (DAD) and an electrospray ionization mass spectrometer was developed for an accurate and reliable quality assessment. Materials and Methods: Eight compounds were qualitative identified based on their mass spectra and by comparing with standard compounds and quantitative analyzed by HPLC-DAD. Separation of eight compounds was carried out on a LUNA C 18 column (S-5 μm, 4.6 mm i.d. ×250 mm) with gradient elution composed of acetonitrile and 0.1% trifluoroacetic acid. Results: The data showed good linearity (R2 > 0.9996). The limits of detection and the limits of quantification were <0.53 μg and 1.62 μg, respectively. Inter- and Intra-day precisions (expressed as relative standard deviation values) were within 1.94% and 1.91%, respectively. The recovery of the method was in the range of 94.24-107.90%. Conclusion: The established method is effective and could be applied to quality control of Samsoeum. |
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